Academic Journal
Kinesin‐14 is Important for Chromosome Segregation During Mitosis and Meiosis in the Ciliate Tetrahymena thermophila
| العنوان: | Kinesin‐14 is Important for Chromosome Segregation During Mitosis and Meiosis in the Ciliate Tetrahymena thermophila |
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| المؤلفون: | Kushida, Yasuharu, Takaine, Masak, Nakano, Kentaro, Sugai, Toshiro, Vasudevan, Krishna Kumar, Guha, Mayukh, Jiang, Yu‐Yang, Gaertig, Jacek, Numata, Osamu |
| المساهمون: | National Institutes of Health, Sumitomo Foundation, Novartis Foundation, University of Tsukuba, Japan Society for the Promotion of Science |
| المصدر: | Journal of Eukaryotic Microbiology ; volume 64, issue 3, page 293-307 ; ISSN 1066-5234 1550-7408 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2016 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Ciliates such as Tetrahymena thermophila have two distinct nuclei within one cell: the micronucleus that undergoes mitosis and meiosis and the macronucleus that undergoes amitosis, a type of nuclear division that does not involve a bipolar spindle, but still relies on intranuclear microtubules. Ciliates provide an opportunity for the discovery of factors that specifically contribute to chromosome segregation based on a bipolar spindle, by identification of factors that affect the micronuclear but not the macronuclear division. Kinesin‐14 is a conserved minus‐end directed microtubule motor that cross‐links microtubules and contributes to the bipolar spindle sizing and organization. Here, we use homologous DNA recombination to knock out genes that encode kinesin‐14 orthologues ( KIN 141 , KIN 142 ) in Tetrahymena . A loss of KIN 141 led to severe defects in the chromosome segregation during both mitosis and meiosis but did not affect amitosis. A loss of KIN 141 altered the shape of the meiotic spindle in a way consistent with the KIN 141's contribution to the organization of the spindle poles. EGFP ‐tagged KIN 141 preferentially accumulated at the spindle poles during the meiotic prophase and metaphase I. Thus, in ciliates, kinesin‐14 is important for nuclear divisions that involve a bipolar spindle. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1111/jeu.12366 |
| الاتاحة: | http://dx.doi.org/10.1111/jeu.12366 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjeu.12366 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jeu.12366 |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.C8CE9E78 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/jeu.12366 |
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