Academic Journal
In vitro antioxidant capacity and phytochemical characterization of Eryngium kotschyi Boiss. [Capacidad antioxidante in vitro y caracterización fitoquímica de Eryngium kotschyi Boiss.]
| العنوان: | In vitro antioxidant capacity and phytochemical characterization of Eryngium kotschyi Boiss. [Capacidad antioxidante in vitro y caracterización fitoquímica de Eryngium kotschyi Boiss.] |
|---|---|
| المؤلفون: | Paşayeva L., Şafak E.K., Arıgün T., Fatullayev H., Tugay O. |
| المساهمون: | Selçuk Üniversitesi |
| بيانات النشر: | Academic Association of Pharmaceutical Sciences from Antofagasta (ASOCIFA) |
| سنة النشر: | 2020 |
| المجموعة: | Selçuk Üniversitesi: SÜ Sosyal Bilimler Enstitüsü - Dergisi Açık Arşivi |
| مصطلحات موضوعية: | ABTS+, Antioxidant DPPH, Eryngium kotschyi, FRAP, LC-MS/MS |
| الوصف: | Context: Eryngium kotschyi is one of the species of Eryngium genus and was endemic to Turkey. It was known, that traditionally uses of this plant in the South-Western part of Turkey for the treatment of various diseases. Aims: To evaluate the antioxidant capacities of methanol extract and ethyl acetate, n-butanol and water sub-extracts as well as to determine the phytochemical composition and to quantify the major antioxidant compounds in the active sub-extract. Methods: Besides the total phenolic compounds and total flavonoid compounds, the antioxidant capacity of E. kotschyi was evaluated by DPPH., ABTS+. and FRAP methods and qualitative and quantitative determination of phytochemical constituents in active sub-extract by LC-MS/MS. Results: The highest total phenolic (173.710 ± 1.088 mg gallic acid equivalent/gextract) and total flavonoid content (86.978 ± 0.650 mg catechin equivalent/gextract) was found in ethyl acetate sub-extract of E. kotschyi (EKE) and this sub-extract showed the highest antioxidant capacity on DPPH. (IC50 = 0.264 ± 0.040 mg/mL), ABTS+. (at a concentration of 0.125 mg/mL equivalent to 0.497 µM Trolox) and FRAP (at a concentration of 1 mg/mL equivalent to 1476 ± 5.292 mmol Fe2+) tests. Preliminary examination of the mass spectrums revealed the presence of 24 phytochemical compounds in this sub-extract and 7 of them was quantified. According to quantitative analyses the main compounds of EKE sub-extract were rosmarinic acid (490.820 ± 0.703 µg/mgextract), chlorogenic acid (80.405 ± 0.170 µg/mgextract), isorhamnetin 3-O-rutinoside (72.280 ± 0.33 µg/mgextract), rutin (63.020 ± 0.052 µg/mgextract) and caffeic acid (55.153 ± 0.523 µg/mgextract). Conclusions: The data suggest that EKE possess utilizable antioxidant properties in vitro. The antioxidant capacity of this sub-extract could be due to high content of phenolic and flavonoid contents. © 2020 Journal of Pharmacy & Pharmacognosy Research ; Firat University Scientific Research Projects Management Unit: TLO-2019-9411 Firat University ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | Journal of Pharmacy and Pharmacognosy Research; Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı; 7194250; https://hdl.handle.net/20.500.12395/38631; 18; 31 |
| الاتاحة: | https://hdl.handle.net/20.500.12395/38631 |
| Rights: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.C2C92ACD |
| قاعدة البيانات: | BASE |
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