Academic Journal
Simultaneous Assessment of Cardiac Inflammation and Extracellular Matrix Remodeling after Myocardial Infarction
| العنوان: | Simultaneous Assessment of Cardiac Inflammation and Extracellular Matrix Remodeling after Myocardial Infarction |
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| المؤلفون: | Ramos, Isabel, Henningsson, Markus Olof Samuel, Nezafat, Maryam, Lavin Plaza, Begona, Lorrio Gonzalez, Silvia, Gebhardt, Pierre Klaus, Protti, Andrea, Eykyn, Thomas Roger, Andia, Marcelo E, Flögel, Ulrich, Phinikaridou, Alkystis, Shah, Ajay Manmohan, Botnar, Rene Michael |
| المصدر: | Ramos , I , Henningsson , M O S , Nezafat , M , Lavin Plaza , B , Lorrio Gonzalez , S , Gebhardt , P K , Protti , A , Eykyn , T R , Andia , M E , Flögel , U , Phinikaridou , A , Shah , A M & Botnar , R M 2018 , ' Simultaneous Assessment of Cardiac Inflammation and Extracellular Matrix Remodeling after Myocardial Infarction ' , Circulation-Cardiovascular imaging , vol. 11 , no. 11 , e007453 . https://doi.org/10.1161/CIRCIMAGING.117.007453 |
| سنة النشر: | 2018 |
| المجموعة: | King's College, London: Research Portal |
| الوصف: | BACKGROUND: Optimal healing of the myocardium following myocardial infarction (MI) requires a suitable degree of inflammation and its timely resolution, together with a well-orchestrated deposition and degradation of extracellular matrix (ECM) proteins. METHODS AND RESULTS: MI and SHAM-operated animals were imaged at 3,7,14 and 21 days with 3T magnetic resonance imaging (MRI) using a 19F/1H surface coil. Mice were injected with 19F-perfluorocarbon (PFC) nanoparticles to study inflammatory cell recruitment, and with a gadolinium-based elastin-binding contrast agent (Gd-ESMA) to evaluate elastin content. 19F MRI signal co-localized with infarction areas, as confirmed by late-gadolinium enhancement, and was highest 7days post-MI, correlating with macrophage content (MAC-3 immunohistochemistry) (ρ=0.89,P<0.0001). 19F quantification with in vivo (MRI) and ex vivo nuclear magnetic resonance (NMR) spectroscopy correlated linearly (ρ=0.58,P=0.020). T1 mapping after Gd-ESMA injection showed increased relaxation rate (R1) in the infarcted regions and was significantly higher at 21days compared with 7days post-MI (R1[s-1]:21days=2.8 [IQR,2.69-3.30] vs 7days=2.3 [IQR,2.12-2.5], P<0.05), which agreed with an increased tropoelastin content (ρ=0.89, P<0.0001). The predictive value of each contrast agent for beneficial remodeling was evaluated in a longitudinal proof-of-principle study. Neither R1 nor 19F at day 7 were significant predictors for beneficial remodeling (P=0.68;P=0.062). However, the combination of both measurements (R1<2.34Hz and 0.55≤19F≤1.85) resulted in an odds ratio of 30.0 (CI95%:1.41-638.15;P=0.029) for favorable post-MI remodeling. CONCLUSIONS: Multinuclear 1H/19F MRI allows the simultaneous assessment of inflammation and elastin remodeling in a murine MI model. The interplay of these biological processes affects cardiac outcome and may have potential for improved diagnosis and personalized treatment. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://kclpure.kcl.ac.uk/portal/en/publications/260f6cbf-aefa-437d-9b69-6f88c303d654 |
| DOI: | 10.1161/CIRCIMAGING.117.007453 |
| DOI: | 10.1161/CIRCIMAGING.117.007453?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%3dpubmed |
| الاتاحة: | https://kclpure.kcl.ac.uk/portal/en/publications/260f6cbf-aefa-437d-9b69-6f88c303d654 https://doi.org/10.1161/CIRCIMAGING.117.007453 https://kclpure.kcl.ac.uk/ws/files/103182755/CIRCIMAGING.117.007453.pdf https://www.ahajournals.org/doi/full/10.1161/CIRCIMAGING.117.007453?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%3dpubmed |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.BED42B08 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1161/CIRCIMAGING.117.007453 |
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