Academic Journal
Short-term cold exposure supports human Treg induction in vivo .
| العنوان: | Short-term cold exposure supports human Treg induction in vivo . |
|---|---|
| المؤلفون: | Becker, M., Serr, I., Salb, V.K., Ott, V., Mengel, L., Blüher, M., Weigmann, B., Hauner, H., Tschöp, M.H., Daniel, C. |
| المصدر: | Mol. Metab. 28, 73-82 (2019) |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2019 |
| المجموعة: | PuSH - Publikationsserver des Helmholtz Zentrums München |
| مصطلحات موضوعية: | Regulatory T Cell, Human Adipose Tissue, Beta3-adrenergic Stimulation, Mirabegron, Immunometabolism, Humanized Mice |
| الوصف: | Objective: Obesity and type-2 diabetes (T2D) are metabolic diseases that represent a critical health problem worldwide. Metabolic disease is differentially associated with fat distribution, while visceral white adipose tissue (VAT) is particularly prone to obesity-associated inflammation. Next to their canonical function of immune suppression, regulatory T cells (Tregs) are key in controlling adipose tissue homeostasis. Towards understanding the molecular underpinnings of metabolic disease, we focus on how environmental-metabolic stimuli impinge on the functional interplay between Tregs and adipose tissue. Here, cold exposure or beta3-adrenergic signaling are a promising tool to increase energy expenditure by activating brown adipose tissue, as well as by reducing local inflammation within fat depots by supporting immunosuppressive Tregs. However, in humans, the underlying mechanisms that enable the environmental-immune crosstalk in the periphery and in the respective tissue remain currently unknown.Methods: We used combinatorial approaches of next generation humanized mouse models and in vitro and in vivo experiments together with beta3-adrenergic stimulation to dissect the underlying mechanisms of human Treg induction exposed to environmental stimuli such as cold. To test the translational relevance of our findings, we analyzed samples from the FREECE study in which human subjects were exposed to individualized cooling protocols. Samples were analyzed ex vivo and after in vitro Treg induction using qRT-PCR, immunofluorescence, as well as with multicolor flow cytometry and cell sorting.Results: In vivo application of the beta3-adrenergic receptor agonist mirabegron in humanized mice induced thermogenesis and improved the Treg induction capacity of naive T cells isolated from these animals. Using samples from the human FREECE study, we demonstrate that a short-term cold stimulus supports human Treg induction in vitro and in vivo. Mechanistically, we identify BORCS6 encoding the Ragulator-interacting protein ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2212-8778 |
| Relation: | info:eu-repo/semantics/altIdentifier/wos/WOS:000487684000007; info:eu-repo/semantics/altIdentifier/isbn/2212-8778; info:eu-repo/semantics/altIdentifier/pissn/2212-8778; info:eu-repo/seman; https://push-zb.helmholtz-muenchen.de/frontdoor.php?source_opus=56776; urn:isbn:2212-8778; urn:issn:2212-8778 |
| DOI: | 10.1016/j.molmet.2019.08.002 |
| الاتاحة: | https://push-zb.helmholtz-muenchen.de/frontdoor.php?source_opus=56776 https://doi.org/10.1016/j.molmet.2019.08.002 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.B54BC3E8 |
| قاعدة البيانات: | BASE |
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