Academic Journal
Direct competitive assay for HER2 detection in human plasma using Bloch surface wave-based biosensors
| العنوان: | Direct competitive assay for HER2 detection in human plasma using Bloch surface wave-based biosensors |
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| المؤلفون: | Pileri, Tommaso, Sinibaldi, Alberto, Occhicone, Agostino, Danz, Norbert, Giordani, Elena, Allegretti, Matteo, Sonntag, Frank, Munzert, Peter, Giacomini, Patrizio, Michelotti, Francesco |
| المساهمون: | Pileri, Tommaso, Sinibaldi, Alberto, Occhicone, Agostino, Danz, Norbert, Giordani, Elena, Allegretti, Matteo, Sonntag, Frank, Munzert, Peter, Giacomini, Patrizio, Michelotti, Francesco |
| سنة النشر: | 2023 |
| المجموعة: | Sapienza Università di Roma: CINECA IRIS |
| مصطلحات موضوعية: | Biomarker, Biosensor, Bloch surface wave, HER2, Photonic crystals |
| الوصف: | The overexpression and/or amplification of the HER2/neu oncogene has been proposed as a prognostic marker in breast cancer. The detection of the related peptide HER2 remains a grand challenge in cancer diagnosis and for therapeutic decision-making. Here, we used a biosensing device based on Bloch Surface Waves excited on a one-dimensional photonic crystal (1DPC) as valid alternative to standard techniques. The 1DPC was optimized to operate in the visible spectrum and the biosensor optics has been designed to combine label-free and fluorescence operation modes. This feature enables a real-time monitoring of a direct competitive assay using detection mAbs conjugated with quantum dots for an accurate discrimination in fluorescence mode between HER2-positive/negative human plasma samples. Such a competitive assay was implemented using patterned alternating areas where HER2-Fc chimera and reference molecules were bio-conjugated and monitored in a multiplexed way. By combining Label-Free and fluorescence detection analysis, we were able to tune the parameters of the assay and provide an HER2 detection in human plasma in less than 20min, allowing for a cost-effective assay and rapid turnaround time. The proposed approach offers a promising technique capable of performing combined label-free and fluorescence detection for both diagnosis and therapeutic monitoring of diseases. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/37914005; volume:684; firstpage:115374; journal:ANALYTICAL BIOCHEMISTRY; https://hdl.handle.net/11573/1694207; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85175473400 |
| DOI: | 10.1016/j.ab.2023.115374 |
| الاتاحة: | https://hdl.handle.net/11573/1694207 https://doi.org/10.1016/j.ab.2023.115374 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.B4794D6 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.ab.2023.115374 |
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