Academic Journal

Oral vaccination against bubonic plague using a live avirulent Yersinia pseudotuberculosis strain

التفاصيل البيبلوغرافية
العنوان: Oral vaccination against bubonic plague using a live avirulent Yersinia pseudotuberculosis strain
المؤلفون: Blisnick, Thierry, Avé, Patrick, Huerre, Michel, Carniel, Elisabeth, Demeure, Christian E.
المساهمون: Yersinia, Institut Pasteur Paris (IP), Histotechnologie et Pathologie, This study was funded by an Action Concertée Incitative Microbiologie 2003 fund from the French Ministry of Health.
المصدر: ISSN: 0019-9567.
بيانات النشر: HAL CCSD
American Society for Microbiology
سنة النشر: 2008
المجموعة: Institut Pasteur: HAL
مصطلحات موضوعية: Yersinia, pestis, pseudotuberculosis, plague, vaccine, live, avirulent, MESH: Administration, Oral, MESH: Animals, MESH: Yersinia pestis, MESH: Yersinia pseudotuberculosis, MESH: Antibodies, Bacterial, MESH: Bacterial Proteins, MESH: Blood, MESH: DNA, MESH: Female, MESH: Fimbriae, MESH: Humans, MESH: Liver, MESH: Lymph Nodes, MESH: Mice, MESH: Plague, MESH: Polymerase Chain Reaction, MESH: Spleen, MESH: Survival Analysis, MESH: Vaccines, Attenuated, MESH: Virulence Factors
الوصف: International audience ; We evaluated the possibility of using Yersinia pseudotuberculosis as a live vaccine against plague because it shares high genetic identity with Y. pestis while being much less virulent, genetically much more stable, and deliverable orally. A total of 41 Y. pseudotuberculosis strains were screened by PCR for the absence of the high pathogenicity island, the superantigens YPM, and the type IV pilus and the presence of the pYV virulence plasmid. One strain (IP32680) fulfilled these criteria. This strain was avirulent in mice upon intragastric or subcutaneous inoculation and persisted for 2 months in the mouse intestine without clinical signs of disease. IP32680 reached the mesenteric lymph nodes, spleen, and liver without causing major histological lesions and was cleared after 13 days. The antibodies produced in vaccinated animals recognized both Y. pseudotuberculosis and Y. pestis antigens efficiently. After a subcutaneous challenge with Y. pestis CO92, bacteria were found in low amounts in the organs and rarely in the blood of vaccinated animals. One oral IP32680 inoculation protected 75% of the mice, and two inoculations induced much higher antibody titers and protected 88% of the mice. Our results thus validate the concept that an attenuated Y. pseudotuberculosis strain can be an efficient, inexpensive, safe, and easy-to-produce live vaccine for oral immunization against bubonic plague.
نوع الوثيقة: article in journal/newspaper
اللغة: English
Relation: info:eu-repo/semantics/altIdentifier/pmid/18505804; pasteur-02075711; https://hal-pasteur.archives-ouvertes.fr/pasteur-02075711; https://hal-pasteur.archives-ouvertes.fr/pasteur-02075711/document; https://hal-pasteur.archives-ouvertes.fr/pasteur-02075711/file/Blisnick%20preprint%202008%20HAL.pdf; PUBMED: 18505804; PUBMEDCENTRAL: PMC2493205
DOI: 10.1128/IAI.00034-08
الاتاحة: https://hal-pasteur.archives-ouvertes.fr/pasteur-02075711
https://hal-pasteur.archives-ouvertes.fr/pasteur-02075711/document
https://hal-pasteur.archives-ouvertes.fr/pasteur-02075711/file/Blisnick%20preprint%202008%20HAL.pdf
https://doi.org/10.1128/IAI.00034-08
Rights: http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess
رقم الانضمام: edsbas.B0EF7F11
قاعدة البيانات: BASE
ResultId 1
Header edsbas
BASE
edsbas.B0EF7F11
839
3
Academic Journal
academicJournal
838.664306640625
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edsbas&AN=edsbas.B0EF7F11&custid=s6537998&authtype=sso
FullText Array ( [Availability] => 0 )
Array ( [0] => Array ( [Url] => https://hal-pasteur.archives-ouvertes.fr/pasteur-02075711# [Name] => EDS - BASE [Category] => fullText [Text] => View record in BASE [MouseOverText] => View record in BASE ) )
Items Array ( [Name] => Title [Label] => Title [Group] => Ti [Data] => Oral vaccination against bubonic plague using a live avirulent Yersinia pseudotuberculosis strain )
Array ( [Name] => Author [Label] => Authors [Group] => Au [Data] => <searchLink fieldCode="AR" term="%22Blisnick%2C+Thierry%22">Blisnick, Thierry</searchLink><br /><searchLink fieldCode="AR" term="%22Avé%2C+Patrick%22">Avé, Patrick</searchLink><br /><searchLink fieldCode="AR" term="%22Huerre%2C+Michel%22">Huerre, Michel</searchLink><br /><searchLink fieldCode="AR" term="%22Carniel%2C+Elisabeth%22">Carniel, Elisabeth</searchLink><br /><searchLink fieldCode="AR" term="%22Demeure%2C+Christian+E%2E%22">Demeure, Christian E.</searchLink> )
Array ( [Name] => Author [Label] => Contributors [Group] => Au [Data] => Yersinia<br />Institut Pasteur Paris (IP)<br />Histotechnologie et Pathologie<br />This study was funded by an Action Concertée Incitative Microbiologie 2003 fund from the French Ministry of Health. )
Array ( [Name] => TitleSource [Label] => Source [Group] => Src [Data] => <i>ISSN: 0019-9567</i>. )
Array ( [Name] => Publisher [Label] => Publisher Information [Group] => PubInfo [Data] => HAL CCSD<br />American Society for Microbiology )
Array ( [Name] => DatePubCY [Label] => Publication Year [Group] => Date [Data] => 2008 )
Array ( [Name] => Subset [Label] => Collection [Group] => HoldingsInfo [Data] => Institut Pasteur: HAL )
Array ( [Name] => Subject [Label] => Subject Terms [Group] => Su [Data] => <searchLink fieldCode="DE" term="%22Yersinia%22">Yersinia</searchLink><br /><searchLink fieldCode="DE" term="%22pestis%22">pestis</searchLink><br /><searchLink fieldCode="DE" term="%22pseudotuberculosis%22">pseudotuberculosis</searchLink><br /><searchLink fieldCode="DE" term="%22plague%22">plague</searchLink><br /><searchLink fieldCode="DE" term="%22vaccine%22">vaccine</searchLink><br /><searchLink fieldCode="DE" term="%22live%22">live</searchLink><br /><searchLink fieldCode="DE" term="%22avirulent%22">avirulent</searchLink><br /><searchLink fieldCode="DE" term="%22MESH%3A+Administration%22">MESH: Administration</searchLink><br /><searchLink fieldCode="DE" term="%22Oral%22">Oral</searchLink><br /><searchLink fieldCode="DE" term="%22MESH%3A+Animals%22">MESH: Animals</searchLink><br /><searchLink fieldCode="DE" term="%22MESH%3A+Yersinia+pestis%22">MESH: Yersinia pestis</searchLink><br /><searchLink fieldCode="DE" term="%22MESH%3A+Yersinia+pseudotuberculosis%22">MESH: Yersinia pseudotuberculosis</searchLink><br /><searchLink fieldCode="DE" term="%22MESH%3A+Antibodies%22">MESH: Antibodies</searchLink><br /><searchLink fieldCode="DE" term="%22Bacterial%22">Bacterial</searchLink><br /><searchLink fieldCode="DE" term="%22MESH%3A+Bacterial+Proteins%22">MESH: Bacterial Proteins</searchLink><br /><searchLink fieldCode="DE" term="%22MESH%3A+Blood%22">MESH: Blood</searchLink><br /><searchLink fieldCode="DE" term="%22MESH%3A+DNA%22">MESH: DNA</searchLink><br /><searchLink fieldCode="DE" term="%22MESH%3A+Female%22">MESH: Female</searchLink><br /><searchLink fieldCode="DE" term="%22MESH%3A+Fimbriae%22">MESH: Fimbriae</searchLink><br /><searchLink fieldCode="DE" term="%22MESH%3A+Humans%22">MESH: Humans</searchLink><br /><searchLink fieldCode="DE" term="%22MESH%3A+Liver%22">MESH: Liver</searchLink><br /><searchLink fieldCode="DE" term="%22MESH%3A+Lymph+Nodes%22">MESH: Lymph Nodes</searchLink><br /><searchLink fieldCode="DE" term="%22MESH%3A+Mice%22">MESH: Mice</searchLink><br /><searchLink fieldCode="DE" term="%22MESH%3A+Plague%22">MESH: Plague</searchLink><br /><searchLink fieldCode="DE" term="%22MESH%3A+Polymerase+Chain+Reaction%22">MESH: Polymerase Chain Reaction</searchLink><br /><searchLink fieldCode="DE" term="%22MESH%3A+Spleen%22">MESH: Spleen</searchLink><br /><searchLink fieldCode="DE" term="%22MESH%3A+Survival+Analysis%22">MESH: Survival Analysis</searchLink><br /><searchLink fieldCode="DE" term="%22MESH%3A+Vaccines%22">MESH: Vaccines</searchLink><br /><searchLink fieldCode="DE" term="%22Attenuated%22">Attenuated</searchLink><br /><searchLink fieldCode="DE" term="%22MESH%3A+Virulence+Factors%22">MESH: Virulence Factors</searchLink> )
Array ( [Name] => Abstract [Label] => Description [Group] => Ab [Data] => International audience ; We evaluated the possibility of using Yersinia pseudotuberculosis as a live vaccine against plague because it shares high genetic identity with Y. pestis while being much less virulent, genetically much more stable, and deliverable orally. A total of 41 Y. pseudotuberculosis strains were screened by PCR for the absence of the high pathogenicity island, the superantigens YPM, and the type IV pilus and the presence of the pYV virulence plasmid. One strain (IP32680) fulfilled these criteria. This strain was avirulent in mice upon intragastric or subcutaneous inoculation and persisted for 2 months in the mouse intestine without clinical signs of disease. IP32680 reached the mesenteric lymph nodes, spleen, and liver without causing major histological lesions and was cleared after 13 days. The antibodies produced in vaccinated animals recognized both Y. pseudotuberculosis and Y. pestis antigens efficiently. After a subcutaneous challenge with Y. pestis CO92, bacteria were found in low amounts in the organs and rarely in the blood of vaccinated animals. One oral IP32680 inoculation protected 75% of the mice, and two inoculations induced much higher antibody titers and protected 88% of the mice. Our results thus validate the concept that an attenuated Y. pseudotuberculosis strain can be an efficient, inexpensive, safe, and easy-to-produce live vaccine for oral immunization against bubonic plague. )
Array ( [Name] => TypeDocument [Label] => Document Type [Group] => TypDoc [Data] => article in journal/newspaper )
Array ( [Name] => Language [Label] => Language [Group] => Lang [Data] => English )
Array ( [Name] => NoteTitleSource [Label] => Relation [Group] => SrcInfo [Data] => info:eu-repo/semantics/altIdentifier/pmid/18505804; pasteur-02075711; https://hal-pasteur.archives-ouvertes.fr/pasteur-02075711; https://hal-pasteur.archives-ouvertes.fr/pasteur-02075711/document; https://hal-pasteur.archives-ouvertes.fr/pasteur-02075711/file/Blisnick%20preprint%202008%20HAL.pdf; PUBMED: 18505804; PUBMEDCENTRAL: PMC2493205 )
Array ( [Name] => DOI [Label] => DOI [Group] => ID [Data] => 10.1128/IAI.00034-08 )
Array ( [Name] => URL [Label] => Availability [Group] => URL [Data] => https://hal-pasteur.archives-ouvertes.fr/pasteur-02075711<br />https://hal-pasteur.archives-ouvertes.fr/pasteur-02075711/document<br />https://hal-pasteur.archives-ouvertes.fr/pasteur-02075711/file/Blisnick%20preprint%202008%20HAL.pdf<br />https://doi.org/10.1128/IAI.00034-08 )
Array ( [Name] => Copyright [Label] => Rights [Group] => Cpyrght [Data] => http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess )
Array ( [Name] => AN [Label] => Accession Number [Group] => ID [Data] => edsbas.B0EF7F11 )
RecordInfo Array ( [BibEntity] => Array ( [Identifiers] => Array ( [0] => Array ( [Type] => doi [Value] => 10.1128/IAI.00034-08 ) ) [Languages] => Array ( [0] => Array ( [Text] => English ) ) [Subjects] => Array ( [0] => Array ( [SubjectFull] => Yersinia [Type] => general ) [1] => Array ( [SubjectFull] => pestis [Type] => general ) [2] => Array ( [SubjectFull] => pseudotuberculosis [Type] => general ) [3] => Array ( [SubjectFull] => plague [Type] => general ) [4] => Array ( [SubjectFull] => vaccine [Type] => general ) [5] => Array ( [SubjectFull] => live [Type] => general ) [6] => Array ( [SubjectFull] => avirulent [Type] => general ) [7] => Array ( [SubjectFull] => MESH: Administration [Type] => general ) [8] => Array ( [SubjectFull] => Oral [Type] => general ) [9] => Array ( [SubjectFull] => MESH: Animals [Type] => general ) [10] => Array ( [SubjectFull] => MESH: Yersinia pestis [Type] => general ) [11] => Array ( [SubjectFull] => MESH: Yersinia pseudotuberculosis [Type] => general ) [12] => Array ( [SubjectFull] => MESH: Antibodies [Type] => general ) [13] => Array ( [SubjectFull] => Bacterial [Type] => general ) [14] => Array ( [SubjectFull] => MESH: Bacterial Proteins [Type] => general ) [15] => Array ( [SubjectFull] => MESH: Blood [Type] => general ) [16] => Array ( [SubjectFull] => MESH: DNA [Type] => general ) [17] => Array ( [SubjectFull] => MESH: Female [Type] => general ) [18] => Array ( [SubjectFull] => MESH: Fimbriae [Type] => general ) [19] => Array ( [SubjectFull] => MESH: Humans [Type] => general ) [20] => Array ( [SubjectFull] => MESH: Liver [Type] => general ) [21] => Array ( [SubjectFull] => MESH: Lymph Nodes [Type] => general ) [22] => Array ( [SubjectFull] => MESH: Mice [Type] => general ) [23] => Array ( [SubjectFull] => MESH: Plague [Type] => general ) [24] => Array ( [SubjectFull] => MESH: Polymerase Chain Reaction [Type] => general ) [25] => Array ( [SubjectFull] => MESH: Spleen [Type] => general ) [26] => Array ( [SubjectFull] => MESH: Survival Analysis [Type] => general ) [27] => Array ( [SubjectFull] => MESH: Vaccines [Type] => general ) [28] => Array ( [SubjectFull] => Attenuated [Type] => general ) [29] => Array ( [SubjectFull] => MESH: Virulence Factors [Type] => general ) ) [Titles] => Array ( [0] => Array ( [TitleFull] => Oral vaccination against bubonic plague using a live avirulent Yersinia pseudotuberculosis strain [Type] => main ) ) ) [BibRelationships] => Array ( [HasContributorRelationships] => Array ( [0] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Blisnick, Thierry ) ) ) [1] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Avé, Patrick ) ) ) [2] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Huerre, Michel ) ) ) [3] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Carniel, Elisabeth ) ) ) [4] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Demeure, Christian E. ) ) ) [5] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Yersinia ) ) ) [6] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Institut Pasteur Paris (IP) ) ) ) [7] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Histotechnologie et Pathologie ) ) ) [8] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => This study was funded by an Action Concertée Incitative Microbiologie 2003 fund from the French Ministry of Health. ) ) ) ) [IsPartOfRelationships] => Array ( [0] => Array ( [BibEntity] => Array ( [Dates] => Array ( [0] => Array ( [D] => 01 [M] => 01 [Type] => published [Y] => 2008 ) ) [Identifiers] => Array ( [0] => Array ( [Type] => issn-locals [Value] => edsbas ) [1] => Array ( [Type] => issn-locals [Value] => edsbas.oa ) ) [Titles] => Array ( [0] => Array ( [TitleFull] => ISSN: 0019-9567 [Type] => main ) ) ) ) ) ) )
IllustrationInfo