Academic Journal
Locus-level L1 DNA methylation profiling reveals the epigenetic and transcriptional interplay between L1s and their integration sites
| العنوان: | Locus-level L1 DNA methylation profiling reveals the epigenetic and transcriptional interplay between L1s and their integration sites |
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| المؤلفون: | Lanciano, Sophie, Philippe, Claude, Sarkar, Arpita, Pratella, David, Domrane, Cécilia, Doucet, Aurélien, J, van Essen, Dominic, Saccani, Simona, Ferry, Laure, Defossez, Pierre-Antoine, Cristofari, Gael |
| المساهمون: | Institut de Recherche sur le Cancer et le Vieillissement (IRCAN), Université Nice Sophia Antipolis (1965 - 2019) (UNS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UniCA), Centre épigénétique et destin cellulaire (EDC), Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Fondation pour la Recherche Médicale (DEQ20180339170), Institut National du Cancer (INCa PLBIO 2020-095), Fondation ARC (PGA1/ RF20180206807), Canceropôle PACA, INCa and the Region Sud (Projet Emergence), INSERM (GOLD Cross-cutting Program on Genomic Variability), CNRS (GDR 3546), ANR-16-CE12-0020,RETROMET,Rendre unique l'ADN répété ou comment révéler la régulation épigénétique des rétrotransposons L1 dans les cellules somatiques humaines à une résolution inégalée.(2016), ANR-21-CE12-0001,ActiveLINE,Activités et diversité des rétrotransposons L1 naturels dans les génomes humains(2021), ANR-15-IDEX-0001,UCA JEDI,Idex UCA JEDI(2015), ANR-11-LABX-0028,SIGNALIFE,Réseau d'Innovation sur les Voies de Signalisation en Sciences de la Vie(2011), ANR-19-CE12-0032,ImpacTE,Réseau de régulation et élément LINE-1 : impact global des éléments transposables récents sur l'activité génique chez les Mammifères(2019), ANR-11-LABX-0071,WHO AM I,Determinants de l'Identité : de la molécule à l'individu(2011), ANR-18-IDEX-0001,Université de Paris,Université de Paris(2018) |
| المصدر: | ISSN: 2666-979X ; Cell Genomics ; https://hal.science/hal-04462176 ; Cell Genomics, 2024, 4 (2), pp.100498. ⟨10.1016/j.xgen.2024.100498⟩. |
| بيانات النشر: | HAL CCSD Elsevier |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | LINE-1, transposable elements, transposon, 5-methyl-cytosine, nanopore, chromatin, YY1, ESR1, estrogen receptor, nuclear receptor, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BDD.EO]Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis, [SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM], [SDV.CAN]Life Sciences [q-bio]/Cancer, [SDV.GEN.GH]Life Sciences [q-bio]/Genetics/Human genetics |
| الوصف: | International audience ; Long interspersed element 1 (L1) retrotransposons are implicated in human disease and evolution. Their global activity is repressed by DNA methylation, but deciphering the regulation of individual copies has been challenging. Here, we combine short- and long-read sequencing to unveil L1 methylation heterogeneity across cell types, families, and individual loci and elucidate key principles involved. We find that the youngest primate L1 families are specifically hypomethylated in pluripotent stem cells and the placenta but not in most tumors. Locally, intronic L1 methylation is intimately associated with gene transcription. Conversely, the L1 methylation state can propagate to the proximal region up to 300 bp. This phenomenon is accompanied by the binding of specific transcription factors, which drive the expression of L1 and chimeric transcripts. Finally, L1 hypomethylation alone is typically insufficient to trigger L1 expression due to redundant silencing pathways. Our results illuminate the epigenetic and transcriptional interplay between retrotransposons and their host genome. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | hal-04462176; https://hal.science/hal-04462176; https://hal.science/hal-04462176/document; https://hal.science/hal-04462176/file/1-s2.0-S2666979X24000259-main.pdf; BIORXIV: 2023.01.03.522582 |
| DOI: | 10.1016/j.xgen.2024.100498 |
| الاتاحة: | https://hal.science/hal-04462176 https://hal.science/hal-04462176/document https://hal.science/hal-04462176/file/1-s2.0-S2666979X24000259-main.pdf https://doi.org/10.1016/j.xgen.2024.100498 |
| Rights: | http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.AFDA02EF |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.xgen.2024.100498 |
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