Dissertation/ Thesis
أعرض في EDS

Vrednotenje toksičnosti kompleksov egerolizinov in proteinov z domeno MACPF za žitnega strgača in kapusovega bolhača ; Assessment of toxicity of aegerolysin and MACPF-domain protein complexes against cereal leaf beetle and cabbage flea beetle

التفاصيل البيبلوغرافية
العنوان: Vrednotenje toksičnosti kompleksov egerolizinov in proteinov z domeno MACPF za žitnega strgača in kapusovega bolhača ; Assessment of toxicity of aegerolysin and MACPF-domain protein complexes against cereal leaf beetle and cabbage flea beetle
المؤلفون: Pavšič, Ana
المساهمون: Razinger, Jaka
المصدر: Ljubljana
بيانات النشر: A. Pavšič
سنة النشر: 2021
المجموعة: University of Ljubljana: Repository (RUJ) / Repozitorij Univerze v Ljubljani
مصطلحات موضوعية: bioinsekticid, ceramid fosfoetanolamin, družina lepencev, egerolizini, kapusov bolhač, Oulema melanopus, Phyllotreta atra, protein z domeno MACPF, testiranje toksičnosti, žitni strgač, bioinsecticide, cabbage flea beetle, ceramide phosphoethanolamine, cereal leaf beetle, Chrysomelidae family, aegerolysins, MACPF protein, toxicity testings, info:eu-repo/classification/udc/577.2(043.2)
الوصف: Potreba po iskanju novih bioinsekticidov je vedno večja. V tej magistrski nalogi smo testirali proteine egerolizinske družine iz bukovega ostrigarja, ki v kombinaciji s pleurotolizinom B, proteinskim partnerjem z domeno MACPF (ang. membrane-attack domain/perforin) deluje insekticidno na koloradskega in koruznega hrošča, ki spadata v družino lepencev (Chrysomelidae). Insekticidno delovanje je posledica vezave egerolizinskega partnerja na membranski sfingolipidni receptor ceramid fosfoetanolamin (CPE), ki je specifičen za nevretenčarje. Da bi preverili, ali so kompleksi na osnovi egerolizinov toksični tudi za druge hrošče iz družine lepencev, smo komplekse egerolizin/pleurotolizin B testirali še na dveh rastlinskih škodljivcih iste družine: na žitnem strgaču (Oulema melanopus) in na kapusovem bolhaču (Phyllotreta atra). Žuželke smo nabrali na netretiranih poljih in nastavili poskus tako, da smo z egerolizini in egerolizinskimi kompleksi obdelali liste, s katerimi so se žuželke prehranjevale. Naši testi so pokazali občutljivost žitnega strgača na izpostavitev proteinom, medtem ko osebki kapusovega bolhača za to niso bili dovzetni. Z analizo lipidnih ekstraktov žuželk smo preverili prisotnost egerolizinskega receptorja CPE pri žitnem strgaču in kapusovem bolhaču kakor tudi pri drugih rastlinskih škodljivcih (marmorirana smrdljivka, plodova vinska mušica, velika žitna uš, koruzni hrošč in koloradski hrošč). Prisotnost CPE smo dokazali v vseh vzorcih, a v različnih koncentracijah. Najvišjo koncentracijo CPE ima plodova vinska mušica, najmanjšo pa velika žitna uš. Primerjava lipidomskih analiz in testov toksičnosti je pokazala, da koncentracija CPE v membranah nima vloge pri delovanju egerolizinov in egerolizinskih kompleksov na žuželke. ; The need to find new bioinsecticides is continuously increasing. In this MSc thesis, we tested aegerolysin proteins from oyster mushrooms that had been found to exert insecticidal effects against two plant pests that belong to the Chrysomelidae family: the Colorado potato beetle and ...
نوع الوثيقة: master thesis
وصف الملف: application/pdf
اللغة: Slovenian
Relation: https://repozitorij.uni-lj.si/IzpisGradiva.php?id=131376; https://repozitorij.uni-lj.si/Dokument.php?id=148687&dn=; https://repozitorij.uni-lj.si/Dokument.php?id=148688&dn=; https://repozitorij.uni-lj.si/Dokument.php?id=148689&dn=; https://repozitorij.uni-lj.si/Dokument.php?id=148690&dn=; https://plus.si.cobiss.net/opac7/bib/78160643?lang=sl
الاتاحة: https://repozitorij.uni-lj.si/IzpisGradiva.php?id=131376
https://repozitorij.uni-lj.si/Dokument.php?id=148687&dn=
https://repozitorij.uni-lj.si/Dokument.php?id=148688&dn=
https://repozitorij.uni-lj.si/Dokument.php?id=148689&dn=
https://repozitorij.uni-lj.si/Dokument.php?id=148690&dn=
https://plus.si.cobiss.net/opac7/bib/78160643?lang=sl
Rights: info:eu-repo/semantics/openAccess
رقم الانضمام: edsbas.AF53E4E4
قاعدة البيانات: BASE
ResultId 1
Header edsbas
BASE
edsbas.AF53E4E4
841
3
Dissertation/ Thesis
dissertation
840.946350097656
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edsbas&AN=edsbas.AF53E4E4&custid=s6537998&authtype=sso
FullText Array ( [Availability] => 0 )
Array ( [0] => Array ( [Url] => https://repozitorij.uni-lj.si/IzpisGradiva.php?id=131376# [Name] => EDS - BASE [Category] => fullText [Text] => View record in BASE [MouseOverText] => View record in BASE ) )
Items Array ( [Name] => Title [Label] => Title [Group] => Ti [Data] => Vrednotenje toksičnosti kompleksov egerolizinov in proteinov z domeno MACPF za žitnega strgača in kapusovega bolhača ; Assessment of toxicity of aegerolysin and MACPF-domain protein complexes against cereal leaf beetle and cabbage flea beetle )
Array ( [Name] => Author [Label] => Authors [Group] => Au [Data] => <searchLink fieldCode="AR" term="%22Pavšič%2C+Ana%22">Pavšič, Ana</searchLink> )
Array ( [Name] => Author [Label] => Contributors [Group] => Au [Data] => Razinger, Jaka )
Array ( [Name] => TitleSource [Label] => Source [Group] => Src [Data] => Ljubljana )
Array ( [Name] => Publisher [Label] => Publisher Information [Group] => PubInfo [Data] => A. Pavšič )
Array ( [Name] => DatePubCY [Label] => Publication Year [Group] => Date [Data] => 2021 )
Array ( [Name] => Subset [Label] => Collection [Group] => HoldingsInfo [Data] => University of Ljubljana: Repository (RUJ) / Repozitorij Univerze v Ljubljani )
Array ( [Name] => Subject [Label] => Subject Terms [Group] => Su [Data] => <searchLink fieldCode="DE" term="%22bioinsekticid%22">bioinsekticid</searchLink><br /><searchLink fieldCode="DE" term="%22ceramid+fosfoetanolamin%22">ceramid fosfoetanolamin</searchLink><br /><searchLink fieldCode="DE" term="%22družina+lepencev%22">družina lepencev</searchLink><br /><searchLink fieldCode="DE" term="%22egerolizini%22">egerolizini</searchLink><br /><searchLink fieldCode="DE" term="%22kapusov+bolhač%22">kapusov bolhač</searchLink><br /><searchLink fieldCode="DE" term="%22Oulema+melanopus%22">Oulema melanopus</searchLink><br /><searchLink fieldCode="DE" term="%22Phyllotreta+atra%22">Phyllotreta atra</searchLink><br /><searchLink fieldCode="DE" term="%22protein+z+domeno+MACPF%22">protein z domeno MACPF</searchLink><br /><searchLink fieldCode="DE" term="%22testiranje+toksičnosti%22">testiranje toksičnosti</searchLink><br /><searchLink fieldCode="DE" term="%22žitni+strgač%22">žitni strgač</searchLink><br /><searchLink fieldCode="DE" term="%22bioinsecticide%22">bioinsecticide</searchLink><br /><searchLink fieldCode="DE" term="%22cabbage+flea+beetle%22">cabbage flea beetle</searchLink><br /><searchLink fieldCode="DE" term="%22ceramide+phosphoethanolamine%22">ceramide phosphoethanolamine</searchLink><br /><searchLink fieldCode="DE" term="%22cereal+leaf+beetle%22">cereal leaf beetle</searchLink><br /><searchLink fieldCode="DE" term="%22Chrysomelidae+family%22">Chrysomelidae family</searchLink><br /><searchLink fieldCode="DE" term="%22aegerolysins%22">aegerolysins</searchLink><br /><searchLink fieldCode="DE" term="%22MACPF+protein%22">MACPF protein</searchLink><br /><searchLink fieldCode="DE" term="%22toxicity+testings%22">toxicity testings</searchLink><br /><searchLink fieldCode="DE" term="%22info%3Aeu-repo%2Fclassification%2Fudc%2F577%2E2%28043%2E2%29%22">info:eu-repo/classification/udc/577.2(043.2)</searchLink> )
Array ( [Name] => Abstract [Label] => Description [Group] => Ab [Data] => Potreba po iskanju novih bioinsekticidov je vedno večja. V tej magistrski nalogi smo testirali proteine egerolizinske družine iz bukovega ostrigarja, ki v kombinaciji s pleurotolizinom B, proteinskim partnerjem z domeno MACPF (ang. membrane-attack domain/perforin) deluje insekticidno na koloradskega in koruznega hrošča, ki spadata v družino lepencev (Chrysomelidae). Insekticidno delovanje je posledica vezave egerolizinskega partnerja na membranski sfingolipidni receptor ceramid fosfoetanolamin (CPE), ki je specifičen za nevretenčarje. Da bi preverili, ali so kompleksi na osnovi egerolizinov toksični tudi za druge hrošče iz družine lepencev, smo komplekse egerolizin/pleurotolizin B testirali še na dveh rastlinskih škodljivcih iste družine: na žitnem strgaču (Oulema melanopus) in na kapusovem bolhaču (Phyllotreta atra). Žuželke smo nabrali na netretiranih poljih in nastavili poskus tako, da smo z egerolizini in egerolizinskimi kompleksi obdelali liste, s katerimi so se žuželke prehranjevale. Naši testi so pokazali občutljivost žitnega strgača na izpostavitev proteinom, medtem ko osebki kapusovega bolhača za to niso bili dovzetni. Z analizo lipidnih ekstraktov žuželk smo preverili prisotnost egerolizinskega receptorja CPE pri žitnem strgaču in kapusovem bolhaču kakor tudi pri drugih rastlinskih škodljivcih (marmorirana smrdljivka, plodova vinska mušica, velika žitna uš, koruzni hrošč in koloradski hrošč). Prisotnost CPE smo dokazali v vseh vzorcih, a v različnih koncentracijah. Najvišjo koncentracijo CPE ima plodova vinska mušica, najmanjšo pa velika žitna uš. Primerjava lipidomskih analiz in testov toksičnosti je pokazala, da koncentracija CPE v membranah nima vloge pri delovanju egerolizinov in egerolizinskih kompleksov na žuželke. ; The need to find new bioinsecticides is continuously increasing. In this MSc thesis, we tested aegerolysin proteins from oyster mushrooms that had been found to exert insecticidal effects against two plant pests that belong to the Chrysomelidae family: the Colorado potato beetle and ... )
Array ( [Name] => TypeDocument [Label] => Document Type [Group] => TypDoc [Data] => master thesis )
Array ( [Name] => Format [Label] => File Description [Group] => SrcInfo [Data] => application/pdf )
Array ( [Name] => Language [Label] => Language [Group] => Lang [Data] => Slovenian )
Array ( [Name] => NoteTitleSource [Label] => Relation [Group] => SrcInfo [Data] => https://repozitorij.uni-lj.si/IzpisGradiva.php?id=131376; https://repozitorij.uni-lj.si/Dokument.php?id=148687&dn=; https://repozitorij.uni-lj.si/Dokument.php?id=148688&dn=; https://repozitorij.uni-lj.si/Dokument.php?id=148689&dn=; https://repozitorij.uni-lj.si/Dokument.php?id=148690&dn=; https://plus.si.cobiss.net/opac7/bib/78160643?lang=sl )
Array ( [Name] => URL [Label] => Availability [Group] => URL [Data] => https://repozitorij.uni-lj.si/IzpisGradiva.php?id=131376<br />https://repozitorij.uni-lj.si/Dokument.php?id=148687&dn=<br />https://repozitorij.uni-lj.si/Dokument.php?id=148688&dn=<br />https://repozitorij.uni-lj.si/Dokument.php?id=148689&dn=<br />https://repozitorij.uni-lj.si/Dokument.php?id=148690&dn=<br />https://plus.si.cobiss.net/opac7/bib/78160643?lang=sl )
Array ( [Name] => Copyright [Label] => Rights [Group] => Cpyrght [Data] => info:eu-repo/semantics/openAccess )
Array ( [Name] => AN [Label] => Accession Number [Group] => ID [Data] => edsbas.AF53E4E4 )
RecordInfo Array ( [BibEntity] => Array ( [Languages] => Array ( [0] => Array ( [Text] => Slovenian ) ) [Subjects] => Array ( [0] => Array ( [SubjectFull] => bioinsekticid [Type] => general ) [1] => Array ( [SubjectFull] => ceramid fosfoetanolamin [Type] => general ) [2] => Array ( [SubjectFull] => družina lepencev [Type] => general ) [3] => Array ( [SubjectFull] => egerolizini [Type] => general ) [4] => Array ( [SubjectFull] => kapusov bolhač [Type] => general ) [5] => Array ( [SubjectFull] => Oulema melanopus [Type] => general ) [6] => Array ( [SubjectFull] => Phyllotreta atra [Type] => general ) [7] => Array ( [SubjectFull] => protein z domeno MACPF [Type] => general ) [8] => Array ( [SubjectFull] => testiranje toksičnosti [Type] => general ) [9] => Array ( [SubjectFull] => žitni strgač [Type] => general ) [10] => Array ( [SubjectFull] => bioinsecticide [Type] => general ) [11] => Array ( [SubjectFull] => cabbage flea beetle [Type] => general ) [12] => Array ( [SubjectFull] => ceramide phosphoethanolamine [Type] => general ) [13] => Array ( [SubjectFull] => cereal leaf beetle [Type] => general ) [14] => Array ( [SubjectFull] => Chrysomelidae family [Type] => general ) [15] => Array ( [SubjectFull] => aegerolysins [Type] => general ) [16] => Array ( [SubjectFull] => MACPF protein [Type] => general ) [17] => Array ( [SubjectFull] => toxicity testings [Type] => general ) [18] => Array ( [SubjectFull] => info:eu-repo/classification/udc/577.2(043.2) [Type] => general ) ) [Titles] => Array ( [0] => Array ( [TitleFull] => Vrednotenje toksičnosti kompleksov egerolizinov in proteinov z domeno MACPF za žitnega strgača in kapusovega bolhača ; Assessment of toxicity of aegerolysin and MACPF-domain protein complexes against cereal leaf beetle and cabbage flea beetle [Type] => main ) ) ) [BibRelationships] => Array ( [HasContributorRelationships] => Array ( [0] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Pavšič, Ana ) ) ) [1] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Razinger, Jaka ) ) ) ) [IsPartOfRelationships] => Array ( [0] => Array ( [BibEntity] => Array ( [Dates] => Array ( [0] => Array ( [D] => 01 [M] => 01 [Type] => published [Y] => 2021 ) ) [Identifiers] => Array ( [0] => Array ( [Type] => issn-locals [Value] => edsbas ) [1] => Array ( [Type] => issn-locals [Value] => edsbas.oa ) ) [Titles] => Array ( [0] => Array ( [TitleFull] => Ljubljana [Type] => main ) ) ) ) ) ) )
IllustrationInfo