التفاصيل البيبلوغرافية
| العنوان: |
The Dynamic Range of Acidity: Tracking Rules for the Unidirectional Penetration of Cellular Compartments |
| المؤلفون: |
Assies, Lea, Mercier, Vincent, Lopez Andarias, Javier, Roux, Aurélien, Sakai, Naomi, Matile, Stefan |
| المصدر: |
ISSN: 1439-4227 ; ChemBioChem, vol. 23, no. 15 (2022) e202200192. |
| سنة النشر: |
2022 |
| المجموعة: |
Université de Genève: Archive ouverte UNIGE |
| مصطلحات موضوعية: |
info:eu-repo/classification/ddc/540, Cell-penetrating peptides, Dynamic acidity, Early endosomes, PH gradients, Trackers, Transmembrane translocation |
| الوصف: |
Labeled ammonium cations with p K a ∼7.4 accumulate in acidic organelles because they can be neutralized transiently to cross the membrane at cytosolic pH 7.2 but not at their internal pH<5.5. Retention in early endosomes with less acidic internal pH was achieved recently using weaker acids of up to p K a 9.8. We report here that primary ammonium cations with higher p K a 10.6, label early endosomes more efficiently. This maximized early endosome tracking coincides with increasing labeling of Golgi networks with similarly weak internal acidity. Guanidinium cations with p K a 13.5 cannot cross the plasma membrane in monomeric form and label the plasma membrane with selectivity for vesicles embarking into endocytosis. Self-assembled into micelles, guanidinium cations enter cells like arginine-rich cell-penetrating peptides and, driven by their membrane potential, penetrate mitochondria unidirectionally despite their high inner pH. The resulting tracking rules with an approximated dynamic range of p K a change ∼3.5 are expected to be generally valid, thus enabling the design of chemistry tools for biology research in the broadest sense. From a practical point of view, most relevant are two complementary fluorescent flipper probes that can be used to image the mechanics at the very beginning of endocytosis. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/35535626; info:eu-repo/semantics/dataset/url/https://doi.org/10.5281/zenodo.6496765; https://archive-ouverte.unige.ch/unige:162697; unige:162697 |
| الاتاحة: |
https://archive-ouverte.unige.ch/unige:162697 |
| Rights: |
info:eu-repo/semantics/openAccess |
| رقم الانضمام: |
edsbas.A4424AEA |
| قاعدة البيانات: |
BASE |