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3D bioartificial stretchable scaffolds mimicking the mechanical hallmarks of human cardiac fibrotic tissue

التفاصيل البيبلوغرافية
العنوان: 3D bioartificial stretchable scaffolds mimicking the mechanical hallmarks of human cardiac fibrotic tissue
المؤلفون: Mattia Spedicati, Francesca Tivano, Alice Zoso, Janira Bei, Mario Lavella, Irene Carmagnola, Valeria Chiono
المساهمون: Spedicati, Mattia, Tivano, Francesca, Zoso, Alice, Bei, Janira, Lavella, Mario, Carmagnola, Irene, Chiono, Valeria
بيانات النشر: ACC SCIENCE
سنة النشر: 2024
المجموعة: PORTO@iris (Publications Open Repository TOrino - Politecnico di Torino)
مصطلحات موضوعية: Human cardiac fibrotic tissues are characterized by a higher stiffness relative to healthy cardiac tissues. These tissues are unable to spontaneously contract and are subjected to passive mechanical stimulation during heart functionality. Moreover, scaffolds that can recapitulate the in vivo mechanical properties of the cardiac fibrotic tissues are lacking. Herein, this study aimed to design and fabricate mechanically stretchable bioartificial scaffolds with biomimetic composition and stiffness comparable to human cardiac fibrotic tissues. Poly(ε-caprolactone) (PCL) scaffolds with a stretchable mesh architecture were initially designed through structural and finite element method (FEM) analyses and subsequently fabricated by melt extrusion additive manufacturing (MEX). Scaffolds were surface functionalized by 3,4-dihydroxy-DL-phenylalanine (DOPA) polymerization (polyDOPA) to improve their interaction with natural polymers. Scaffold pores were then filled with different concentrations (5%, 7%, and 10% w/v) of gelatin methacryloyl (GelMA) hydrogels to support in vitro human cardiac fibroblasts (HCFs) 3D culture, thereby producing bioartificial PCL/GelMA scaffolds. Uniaxial tensile mechanical tests in static and dynamic conditions (1 Hz, 22% maximum strain) demonstrated that the bioartificial scaffolds had in vivo-like stretchability and similar stiffness to that of pathological cardiac tissue (tailored as a function of the number of PCL scaffold layers and GelMA hydrogel concentration). In vitro cell tests on bioartificial scaffolds using HCF-embedded GelMA hydrogels under static conditions displayed increasing cell viability, spreading, and cytoskeleton organization with decreasing GelMA hydrogel concentration. Moreover, α-smooth muscle actin (α-SMA)-positive cells were detected after 7 days of culture in static conditions followed by another 7 days of culture in dynamic conditions and not in HCF-loaded scaffolds cultured in static conditions for 14 days. These results suggested that in vitro culture under cyclic mechanical stimulations could induce an HCF phenotypic switch into myofibroblasts.
الوصف: Bioartificial scaffold; Cardiac fibrosis; Stretchable scaffold; GelMA hydrogel
نوع الوثيقة: article in journal/newspaper
اللغة: unknown
Relation: info:eu-repo/semantics/altIdentifier/wos/WOS:001257555300003; numberofpages:21; journal:INTERNATIONAL JOURNAL OF BIOPRINTING; https://hdl.handle.net/11583/2988744; https://www.accscience.com/journal/IJB/articles/online_first/1438
الاتاحة: https://hdl.handle.net/11583/2988744
https://www.accscience.com/journal/IJB/articles/online_first/1438
Rights: info:eu-repo/semantics/openAccess
رقم الانضمام: edsbas.9F35AE30
قاعدة البيانات: BASE
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