Academic Journal
Insecticidal toxins from Bacillus thuringiensis subsp. kenyae: gene cloning and characterization and comparison with B. thuringiensis subsp. kurstaki CryIA(c) toxins
| العنوان: | Insecticidal toxins from Bacillus thuringiensis subsp. kenyae: gene cloning and characterization and comparison with B. thuringiensis subsp. kurstaki CryIA(c) toxins |
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| المؤلفون: | Von Tersch, M A, Robbins, H L, Jany, C S, Johnson, T B |
| المصدر: | Applied and Environmental Microbiology ; volume 57, issue 2, page 349-358 ; ISSN 0099-2240 1098-5336 |
| بيانات النشر: | American Society for Microbiology |
| سنة النشر: | 1991 |
| الوصف: | Genes encoding insecticidal crystal proteins were cloned from three strains of Bacillus thuringiensis subsp. kenyae and two strains of B. thuringiensis subsp. kurstaki. Characterization of the B. thuringiensis subsp. kenyae toxin genes showed that they are most closely related to cryIA(c) from B. thuringiensis subsp. kurstaki. The cloned genes were introduced into Bacillus host strains, and the spectra of insecticidal activities of each Cry protein were determined for six pest lepidopteran insects. CryIA(c) proteins from B. thuringiensis subsp. kenyae are as active as CryIA(c) proteins from B. thuringiensis subsp. kurstaki against Trichoplusia ni, Lymantria dispar, Heliothis zea, and H. virescens but are significantly less active against Plutella xylostella and, in some cases, Ostrinia nubilalis. The sequence of a cryIA(c) gene from B. thuringiensis subsp. kenyae was determined (GenBank M35524) and compared with that of cryIA(c) from B. thuringiensis subsp. kurstaki. The two genes are more than 99% identical and show seven amino acid differences among the predicted sequences of 1,177 amino acids. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1128/aem.57.2.349-358.1991 |
| الاتاحة: | http://dx.doi.org/10.1128/aem.57.2.349-358.1991 https://journals.asm.org/doi/pdf/10.1128/aem.57.2.349-358.1991 |
| Rights: | https://journals.asm.org/non-commercial-tdm-license |
| رقم الانضمام: | edsbas.9E67BE4A |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/aem.57.2.349-358.1991 |
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