Academic Journal
Use of a High-Resolution Melt Assay To Characterize Codon 54 of the cyp51A Gene of Aspergillus fumigatus on a Rotor-Gene 6000 Instrument
| العنوان: | Use of a High-Resolution Melt Assay To Characterize Codon 54 of the cyp51A Gene of Aspergillus fumigatus on a Rotor-Gene 6000 Instrument |
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| المؤلفون: | Tuohy, M. J., Reja, V., Park, S., Perlin, D. S., Wnek, M., Procop, G. W., Yen-Lieberman, B. |
| المصدر: | Antimicrobial Agents and Chemotherapy ; volume 54, issue 5, page 2248-2251 ; ISSN 0066-4804 1098-6596 |
| بيانات النشر: | American Society for Microbiology |
| سنة النشر: | 2010 |
| الوصف: | A high-resolution melt (HRM) assay using a Rotor-Gene 6000 instrument was developed to characterize the codon for glycine 54 in the cyp51A genes from 13 reference isolates and 12 clinical isolates of Aspergillus fumigatus . Mutations in this codon confer reduced susceptibility to itraconazole and posaconazole. The assay is simple to perform, and a result of “wild type” or “mutant” is available after approximately 1 h following DNA extraction using commercially available reagents and conventional primers. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1128/aac.01712-09 |
| DOI: | 10.1128/AAC.01712-09 |
| الاتاحة: | http://dx.doi.org/10.1128/aac.01712-09 https://journals.asm.org/doi/pdf/10.1128/AAC.01712-09 |
| Rights: | https://journals.asm.org/non-commercial-tdm-license |
| رقم الانضمام: | edsbas.9C7E45F3 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/aac.01712-09 |
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