Academic Journal
Two distinct promoter elements in the human rRNA gene identified by linker scanning mutagenesis.
| العنوان: | Two distinct promoter elements in the human rRNA gene identified by linker scanning mutagenesis. |
|---|---|
| المؤلفون: | Haltiner, M M, Smale, S T, Tjian, R |
| المصدر: | Molecular and Cellular Biology ; volume 6, issue 1, page 227-235 ; ISSN 0270-7306 1098-5549 |
| بيانات النشر: | Informa UK Limited |
| سنة النشر: | 1986 |
| الوصف: | A cell-free RNA polymerase I transcription system was used to evaluate the transcription efficiency of 21 linker scanning mutations that span the human rRNA gene promoter. Our analysis revealed the presence of two major control elements, designated the core and upstream elements, that affect the level of transcription initiation. The core element extends from -45 to +18 relative to the RNA start site, and transcription is severely affected (up to 100-fold) by linker scanning mutations in this region. Linker scanning and deletion mutations in the upstream element, located between nucleotides -156 and -107, cause a three- to fivefold reduction in transcription. Under certain reaction conditions, such as the presence of a high ratio of protein to template or supplementation of the reaction with partially purified protein fractions, sequences upstream of the core element can have an even greater effect (20- to 50-fold) on RNA polymerase I transcription. Primer extension analysis showed that RNA synthesized from all of these mutant templates is initiated at the correct in vivo start site. To examine the functional relationship between the core and the upstream region, mutant promoters were constructed that alter the orientation, distance, or multiplicity of these control elements relative to each other. The upstream control element appears to function in only one orientation, and its position relative to the core is constrained within a fairly narrow region. Moreover, multiple core elements in close proximity to each other have an inhibitory effect on transcription. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1128/mcb.6.1.227 |
| DOI: | 10.1128/MCB.6.1.227 |
| الاتاحة: | http://dx.doi.org/10.1128/mcb.6.1.227 https://syndication.highwire.org/content/doi/10.1128/MCB.6.1.227 |
| رقم الانضمام: | edsbas.9A0EF35D |
| قاعدة البيانات: | BASE |
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