Academic Journal
CellCognition: time-resolved phenotype annotation in high-throughput live cell imaging
| العنوان: | CellCognition: time-resolved phenotype annotation in high-throughput live cell imaging |
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| المؤلفون: | Held, M, Schmitz, M H A, Fischer, B, Walter, T, Neumann, B, Olma, M H, Peter, M, Ellenberg, J, Gerlich, D W |
| المصدر: | Held, M; Schmitz, M H A; Fischer, B; Walter, T; Neumann, B; Olma, M H; Peter, M; Ellenberg, J; Gerlich, D W (2010). CellCognition: time-resolved phenotype annotation in high-throughput live cell imaging. Nature Methods, 7(9):747-754. |
| بيانات النشر: | Nature Publishing Group |
| سنة النشر: | 2010 |
| المجموعة: | University of Zurich (UZH): ZORA (Zurich Open Repository and Archive |
| مصطلحات موضوعية: | Institute for Regenerative Medicine (IREM), 610 Medicine & health |
| الوصف: | Fluorescence time-lapse imaging has become a powerful tool to investigate complex dynamic processes such as cell division or intracellular trafficking. Automated microscopes generate time-resolved imaging data at high throughput, yet tools for quantification of large-scale movie data are largely missing. Here we present CellCognition, a computational framework to annotate complex cellular dynamics. We developed a machine-learning method that combines state-of-the-art classification with hidden Markov modeling for annotation of the progression through morphologically distinct biological states. Incorporation of time information into the annotation scheme was essential to suppress classification noise at state transitions and confusion between different functional states with similar morphology. We demonstrate generic applicability in different assays and perturbation conditions, including a candidate-based RNA interference screen for regulators of mitotic exit in human cells. CellCognition is published as open source software, enabling live-cell imaging-based screening with assays that directly score cellular dynamics. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1548-7091 |
| Relation: | https://www.zora.uzh.ch/41479; info:pmid/20693996; urn:issn:1548-7091 |
| DOI: | 10.1038/nmeth.1486 |
| الاتاحة: | https://www.zora.uzh.ch/id/eprint/41479/ https://www.zora.uzh.ch/41479 https://doi.org/10.1038/nmeth.1486 |
| Rights: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.982B69A2 |
| قاعدة البيانات: | BASE |
| تدمد: | 15487091 |
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| DOI: | 10.1038/nmeth.1486 |