Academic Journal
Whole genome sequence analysis of Aeromonas spp. isolated from ready-to-eat seafood: antimicrobial resistance and virulence factors
| العنوان: | Whole genome sequence analysis of Aeromonas spp. isolated from ready-to-eat seafood: antimicrobial resistance and virulence factors |
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| المؤلفون: | Hye-Jeong Lee, Julia E. Storesund, Bjørn-Tore Lunestad, Sunniva Hoel, Jørgen Lerfall, Anita Nordeng Jakobsen |
| المصدر: | Frontiers in Microbiology, Vol 14 (2023) |
| بيانات النشر: | Frontiers Media S.A. |
| سنة النشر: | 2023 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Aeromonas, antimicrobial resistance, virulence factors, mobile genetic elements, whole genome sequences, multilocus phylogenetic analysis, Microbiology, QR1-502 |
| الوصف: | Aeromonas are widespread in aquatic environments and are considered emerging pathogens in humans and animals. Multidrug resistant (MDR) Aeromonas circulating in the aquatic environment and food production chain can potentially disseminate antimicrobial resistance (AMR) to humans via the foodborne route. In this study, we aimed to investigate AMR and virulence factors of 22 Aeromonas strains isolated from ready-to-eat (RTE) seafood. A multilocus phylogenetic analysis (MLPA) using the concatenated sequences of six housekeeping genes (gyrB, rpoD, gyrA, recA, dnaJ, and dnaX) in the 22 Aeromonas genomes and average nucleotide identity (ANI) analysis revealed eight different species; A. caviae, A. dhakensis, A. hydrophila, A. media, A. rivipollensis, A. salmonicida, A. bestiarum, and A. piscicola. The presence of virulence genes, AMR genes and mobile genetic elements (MGEs) in the Aeromonas genomes was predicted using different databases. Our data showed that the genes responsible for adherence and motility (Msh type IV pili, tap type IV pili, polar flagella), type II secretion system (T2SS) and hemolysins were present in all strains, while the genes encoding enterotoxins and type VI secretion system (T6SS) including major effectors were highly prevalent. Multiple AMR genes encoding β-lactamases such as cphA and blaOXA were detected, and the distribution of those genes was species-specific. In addition, the quinolone resistance gene, qnrS2 was found in a IncQ type plasmid of the A. rivopollensis strain A539. Furthermore, we observed the co-localization of a class I integron (intl1) with two AMR genes (sul1 and aadA1), and a Tn521 transposon carrying a mercury operon in A. caviae strain SU4-2. Various MGEs including other transposons and insertion sequence (IS) elements were identified without strongly associating with detected AMR genes or virulence genes. In conclusion, Aeromonas strains in RTE seafood were potentially pathogenic, carrying several virulence-related genes. Aeromonas carrying multiple AMR genes and ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1664-302X |
| Relation: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1175304/full; https://doaj.org/toc/1664-302X; https://doaj.org/article/43df1bde19aa4fcf861fb7005df7e62e |
| DOI: | 10.3389/fmicb.2023.1175304 |
| الاتاحة: | https://doi.org/10.3389/fmicb.2023.1175304 https://doaj.org/article/43df1bde19aa4fcf861fb7005df7e62e |
| رقم الانضمام: | edsbas.8F08004A |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 1664302X |
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| DOI: | 10.3389/fmicb.2023.1175304 |