Academic Journal
Assessing the effect of anesthetic gas mixtures on hyperpolarized 13 C pyruvate metabolism in the rat brain
| العنوان: | Assessing the effect of anesthetic gas mixtures on hyperpolarized 13 C pyruvate metabolism in the rat brain |
|---|---|
| المؤلفون: | Healicon, Richard, Rooney, Catriona H E, Ball, Vicky, Shinozaki, Ayaka, Miller, Jack J, Smart, Sean, Radford-Smith, Daniel, Anthony, Daniel, Tyler, Damian J, Grist, James T |
| المصدر: | Healicon , R , Rooney , C H E , Ball , V , Shinozaki , A , Miller , J J , Smart , S , Radford-Smith , D , Anthony , D , Tyler , D J & Grist , J T 2022 , ' Assessing the effect of anesthetic gas mixtures on hyperpolarized 13 C pyruvate metabolism in the rat brain ' , Magnetic Resonance in Medicine , vol. 88 , no. 3 , pp. 1324-1332 . https://doi.org/10.1002/mrm.29274 |
| سنة النشر: | 2022 |
| المجموعة: | Aarhus University: Research |
| مصطلحات موضوعية: | MRI, brain, hyperpolarized, metabolism |
| الوصف: | PURPOSE: To determine the effect of altering anesthetic oxygen protocols on measurements of cerebral perfusion and metabolism in the rodent brain. METHODS: Seven rats were anesthetized and underwent serial MRI scans with hyperpolarized [1-13 C]pyruvate and perfusion weighted imaging. The anesthetic carrier gas protocol used varied from 100:0% to 90:10% to 60:40% O2 :N2 O. Spectra were quantified with AMARES and perfusion imaging was processed using model-free deconvolution. A 1-way ANOVA was used to compare results across groups, with pairwise t tests performed with correction for multiple comparisons. Spearman's correlation analysis was performed between O2 % and MR measurements. RESULTS: There was a significant increase in bicarbonate:total 13 C carbon and bicarbonate:13 C pyruvate when moving between 100:0 to 90:10 and 100:0 to 60:40 O2 :N2 O % (0.02 ± 0.01 vs. 0.019 ± 0.005 and 0.02 ± 0.01 vs. 0.05 ± 0.02, respectively) and (0.04 ± 0.01 vs. 0.03 ± 0.01 and 0.04 ± 0.01 vs. 0.08 ± 0.02, respectively). There was a significant difference in 13 C pyruvate time to peak when moving between 100:0 to 90:10 and 100:0 to 60:40 O2 :N2 O % (13 ± 2 vs. 10 ± 1 and 13 ± 2 vs. 7.5 ± 0.5 s, respectively) as well as significant differences in cerebral blood flow (CBF) between gas protocols. Significant correlations between bicarbonate:13 C pyruvate and gas protocol (ρ = -0.47), mean transit time and gas protocol (ρ = 0.41) and 13 C pyruvate time-to-peak and cerebral blood flow (ρ = -0.54) were also observed. CONCLUSIONS: These results demonstrate that the detection and quantification of cerebral metabolism and perfusion is dependent on the oxygen protocol used in the anesthetized rodent brain. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://pure.au.dk/portal/da/publications/assessing-the-effect-of-anesthetic-gas-mixtures-on-hyperpolarized-13c-pyruvate-metabolism-in-the-rat-brain(5f7557c7-ebcf-42f2-9822-69b979a317c3).html |
| DOI: | 10.1002/mrm.29274 |
| الاتاحة: | https://pure.au.dk/portal/da/publications/assessing-the-effect-of-anesthetic-gas-mixtures-on-hyperpolarized-13c-pyruvate-metabolism-in-the-rat-brain(5f7557c7-ebcf-42f2-9822-69b979a317c3).html https://doi.org/10.1002/mrm.29274 https://pure.au.dk/ws/files/342164979/Magnetic_Resonance_in_Med_-_2022_-_Healicon.pdf |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.8EC0B0F0 |
| قاعدة البيانات: | BASE |
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METHODS: Seven rats were anesthetized and underwent serial MRI scans with hyperpolarized [1-13 C]pyruvate and perfusion weighted imaging. The anesthetic carrier gas protocol used varied from 100:0% to 90:10% to 60:40% O2 :N2 O. Spectra were quantified with AMARES and perfusion imaging was processed using model-free deconvolution. A 1-way ANOVA was used to compare results across groups, with pairwise t tests performed with correction for multiple comparisons. Spearman's correlation analysis was performed between O2 % and MR measurements. RESULTS: There was a significant increase in bicarbonate:total 13 C carbon and bicarbonate:13 C pyruvate when moving between 100:0 to 90:10 and 100:0 to 60:40 O2 :N2 O % (0.02 ± 0.01 vs. 0.019 ± 0.005 and 0.02 ± 0.01 vs. 0.05 ± 0.02, respectively) and (0.04 ± 0.01 vs. 0.03 ± 0.01 and 0.04 ± 0.01 vs. 0.08 ± 0.02, respectively). 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