التفاصيل البيبلوغرافية
| العنوان: |
Membrane Profiling by Free Flow Electrophoresis and SWATH-MS to Characterize Subcellular Compartment Proteomes in Mesembryanthemum crystallinum |
| المؤلفون: |
Qi Guo, Lei Liu, Won C. Yim, John C. Cushman, Bronwyn J. Barkla |
| المصدر: |
International Journal of Molecular Sciences; Volume 22; Issue 9; Pages: 5020 |
| بيانات النشر: |
Multidisciplinary Digital Publishing Institute |
| سنة النشر: |
2021 |
| المجموعة: |
MDPI Open Access Publishing |
| مصطلحات موضوعية: |
subcellular proteomics, membrane fractionation, membrane proteome, marker proteins, subcellular localization, lipid metabolism, lipid biosynthesis, ATPase, mass spectrometry, peptide library |
| جغرافية الموضوع: |
agris |
| الوصف: |
The study of subcellular membrane structure and function facilitates investigations into how biological processes are divided within the cell. However, work in this area has been hampered by the limited techniques available to fractionate the different membranes. Free Flow Electrophoresis (FFE) allows for the fractionation of membranes based on their different surface charges, a property made up primarily of their varied lipid and protein compositions. In this study, high-resolution plant membrane fractionation by FFE, combined with mass spectrometry-based proteomics, allowed the simultaneous profiling of multiple cellular membranes from the leaf tissue of the plant Mesembryanthemum crystallinum. Comparisons of the fractionated membranes’ protein profile to that of known markers for specific cellular compartments sheds light on the functions of proteins, as well as provides new evidence for multiple subcellular localization of several proteins, including those involved in lipid metabolism. |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
Molecular Plant Sciences; https://dx.doi.org/10.3390/ijms22095020 |
| DOI: |
10.3390/ijms22095020 |
| الاتاحة: |
https://doi.org/10.3390/ijms22095020 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: |
edsbas.84AC2971 |
| قاعدة البيانات: |
BASE |