Academic Journal
SNAP-25 is expressed in islets of Langerhans and is involved in insulin release
| العنوان: | SNAP-25 is expressed in islets of Langerhans and is involved in insulin release |
|---|---|
| المؤلفون: | Sadoul, Karin, Lang, Jochen, Montecucco, C., Weller, U., Regazzi, Romano, Catsicas, S., Wollheim, Claes, Halban, Philippe A. |
| المصدر: | ISSN: 0021-9525 ; The Journal of cell biology, vol. 128, no. 6 (1995) p. 1019-1028. |
| سنة النشر: | 1995 |
| المجموعة: | Université de Genève: Archive ouverte UNIGE |
| مصطلحات موضوعية: | info:eu-repo/classification/ddc/616, Animals, Botulinum Toxins/pharmacology, Calcium/pharmacology, Cells, Cultured, Insulin/ secretion, Islets of Langerhans/ metabolism, Membrane Proteins, Nerve Tissue Proteins/ biosynthesis/drug effects, Rats, Synaptosomal-Associated Protein 25 |
| الوصف: | SNAP-25 is known as a neuron specific molecule involved in the fusion of small synaptic vesicles with the presynaptic plasma membrane. By immunolocalization and Western blot analysis, it is now shown that SNAP-25 is also expressed in pancreatic endocrine cells. Botulinum neurotoxins (BoNT) A and E were used to study the role of SNAP-25 in insulin secretion. These neurotoxins inhibit transmitter release by cleaving SNAP-25 in neurons. Cells from a pancreatic B cell line (HIT) and primary rat islet cells were permeabilized with streptolysin-O to allow toxin entry. SNAP-25 was cleaved by BoNT/A and BoNT/E, resulting in a molecular mass shift of approximately 1 and 3 kD, respectively. Cleavage was accompanied by an inhibition of Ca(++)-stimulated insulin release in both cell types. In HIT cells, a concentration of 30-40 nM BoNT/E gave maximal inhibition of stimulated insulin secretion of approximately 60%, coinciding with essentially complete cleavage of SNAP-25. Half maximal effects in terms of cleavage and inhibition of insulin release were obtained at a concentration of 5-10 nM. The A type toxin showed maximal and half-maximal effects at concentrations of 4 and 2 nM, respectively. In conclusion, the results suggest a role for SNAP-25 in fusion of dense core secretory granules with the plasma membrane in an endocrine cell type- the pancreatic B cell. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/7896868; https://archive-ouverte.unige.ch/unige:9009; unige:9009 |
| الاتاحة: | https://archive-ouverte.unige.ch/unige:9009 |
| Rights: | info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: | edsbas.812B8595 |
| قاعدة البيانات: | BASE |
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