Conference
Uncovering the tissue-specific regulation of iridoid biosynthesis in two chemodiverse Nepeta species: integration of transcriptomics and metabolomics data
| العنوان: | Uncovering the tissue-specific regulation of iridoid biosynthesis in two chemodiverse Nepeta species: integration of transcriptomics and metabolomics data |
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| المؤلفون: | Matekalo, Dragana, Aničić, Neda, Gašić, Uroš, Skorić, Marijana, Šiler, Branislav, Banjanac, Tijana, Nestorović Živković, Jasmina, Dmitrović, Slavica, Filipović, Biljana, Milutinović, Milica, Božunović, Jelena, Petrović, Luka, Todorović, Miloš, Lukić, Tamara, Mišić, Danijela |
| المساهمون: | Peters, Reuben, Zerbe, Philipp, Tantillo, Dean |
| المصدر: | Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis, Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA |
| بيانات النشر: | University of California |
| سنة النشر: | 2023 |
| المجموعة: | RADaR - Digital Repository of Archived Publications Institute for Biological Research "Sinisa Stankovic" / РАДаР - Репозиторијум Архивираних Дигиталних Радова Институт за биолошка истраживања "Синиша Станковић" |
| مصطلحات موضوعية: | Nepeta rtanjensis, Nepeta grandiflora, iridoids, iridoid biosynthesis, nepetalactone biosynthetic pathway genes, glandular trichomes, transcription factors |
| الوصف: | The genus Nepeta is characterized by a fascinating diversity of iridoid compounds. Some species within the genus are characterized by the presence of both iridoid glycosides (IGs) and iridoid aglycones (IAs), while others produce exclusively IGs, or completely lack iridoids. The absence of certain iridoids does not necessarily imply the absence of gene transcripts dedicated to the particular steps of their biosynthetic pathway. Studying the biosynthetic pathways of iridoids in chemodiverse Nepeta species, while taking into account tissue-specific profiles of metabolites and relevant genes expression, may give us further insight into the mechanisms of regulation of the biosynthesis of these compounds. In this work we performed a comparative transcriptomic analysis of glandular trichomes and abraded leaves of Nepeta rtanjensis, an IAs- and IGs –rich species, and of IGs-producing N. grandiflora. The results of this analysis provided us with very informative data that can be used for parallel study of iridoid-related biosynthetic genes (BGs) and transcription factors (TFs) of the two chemodiverse taxa. On the other hand, differentially expressed genes in the investigated tissues, enabled us to generate a list of candidate genes for yet unelucidated steps of the iridoid metabolic pathway. These results combined with state-of-the-art metabolomics data bring us closer to understanding the molecular background of iridoid diversity within the genus Nepeta, and point to the factors involved in the regulation of the two distinct branches of the iridoid biosynthetic pathway, leading to either IGs or IAs. ; Peters R, Zerbe P, Tantillo D, editors. Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis, Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA. Davis: University of California; 2023. p. 82. |
| نوع الوثيقة: | conference object |
| اللغة: | English |
| Relation: | info:eu-repo/grantAgreement/ScienceFundRS/Ideje/7749433/RS//; info:eu-repo/grantAgreement/MESTD/inst-2020/200007/RS//; http://radar.ibiss.bg.ac.rs/handle/123456789/6082; https://radar.ibiss.bg.ac.rs/bitstream/id/17318/Slavica-13.pdf; https://hdl.handle.net/21.15107/rcub_ibiss_6082 |
| الاتاحة: | http://radar.ibiss.bg.ac.rs/handle/123456789/6082 https://radar.ibiss.bg.ac.rs/bitstream/id/17318/Slavica-13.pdf https://hdl.handle.net/21.15107/rcub_ibiss_6082 |
| Rights: | openAccess ; ARR ; © 2023 by the University of California, Davis |
| رقم الانضمام: | edsbas.7DB89DB9 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |