Image
Image_2_Programmed Cell Death 10 Mediated CXCL2-CXCR2 Signaling in Regulating Tumor-Associated Microglia/Macrophages Recruitment in Glioblastoma.pdf
| العنوان: | Image_2_Programmed Cell Death 10 Mediated CXCL2-CXCR2 Signaling in Regulating Tumor-Associated Microglia/Macrophages Recruitment in Glioblastoma.pdf |
|---|---|
| المؤلفون: | Quan Zhang, Junwen Wang, Xiaolong Yao, Sisi Wu, Weidong Tian, Chao Gan, Xueyan Wan, Chao You, Feng Hu, Suojun Zhang, Huaqiu Zhang, Kai Zhao, Kai Shu, Ting Lei |
| سنة النشر: | 2021 |
| المجموعة: | Frontiers: Figshare |
| مصطلحات موضوعية: | Immunology, Applied Immunology (incl. Antibody Engineering, Xenotransplantation and T-cell Therapies), Autoimmunity, Cellular Immunology, Humoural Immunology and Immunochemistry, Immunogenetics (incl. Genetic Immunology), Innate Immunity, Transplantation Immunology, Tumour Immunology, Immunology not elsewhere classified, Genetic Immunology, Animal Immunology, Veterinary Immunology, glioblastoma, CXCR2, CXCl2, SB225002, tumor-associated microglia/macrophages (TAM), programmed cell death 10 (PDCD10) |
| الوصف: | Background Programmed cell death 10 (PDCD10) plays a crucial role in regulating tumor phenotyping, especially in glioblastoma (GBM). Glioma-associated microglia/macrophages (GAMs) in tumor pathological microenvironment contribute to GBM progression. We previously found that the infiltration of GAMs was associated with PDCD10 expression in GBM patients. The present study aims to further explore the regulation of PDCD10 on GAMs in GBM. Methods Overexpression of PDCD10 in human- and murine-GBM cells was established by lentiviral transduction. Cell behaviors and polarization of primary microglia, microglia- and macrophage-like cells were investigated through indirect co-culture with GBM cells in vitro respectively. The PDCD10-induced release of chemokines was identified by a chemokine protein array. The cross-talk between GBM and microglia as well as macrophages was further studied using selective antagonist SB225002. Finally, an orthotopic homograft mouse model was employed to verify the results of in vitro experiments. Results Indirect co-culture with PDCD10-overexpressed GBM cells promoted proliferation and migration of microglia- and macrophage-like cells, and stimulated pro-tumorigenic polarization of primary microglia, microglia- and macrophage-like cells. Pdcd10-upregulated GBM cells triggered a nearly 6-fold increase of CXC motif chemokine ligand 2 (CXCL2) release, which in turn activated CXC chemokine receptor 2 (CXCR2) and downstream Erk1/2 and Akt signaling in primary microglia, microglia- and macrophage-like cells. The blockage of CXCR2 signaling with specific inhibitor (SB225002) abolished microglia- and macrophage-like cell migration induced by PDCD10-upregulated GBM cells. Moreover, Pdcd10-upregulated GL261 cells promoted GAMs recruitment and tumor growth in vivo. Conclusion Our study demonstrates that overexpression of PDCD10 in GBM recruits and activates microglia/macrophages, which in turn promotes tumor progression. CXCL2-CXCR2 signaling mediated by PDCD10 is potentially involved in the crosstalk ... |
| نوع الوثيقة: | still image |
| اللغة: | unknown |
| Relation: | https://figshare.com/articles/figure/Image_2_Programmed_Cell_Death_10_Mediated_CXCL2-CXCR2_Signaling_in_Regulating_Tumor-Associated_Microglia_Macrophages_Recruitment_in_Glioblastoma_pdf/14660169 |
| DOI: | 10.3389/fimmu.2021.637053.s002 |
| الاتاحة: | https://doi.org/10.3389/fimmu.2021.637053.s002 https://figshare.com/articles/figure/Image_2_Programmed_Cell_Death_10_Mediated_CXCL2-CXCR2_Signaling_in_Regulating_Tumor-Associated_Microglia_Macrophages_Recruitment_in_Glioblastoma_pdf/14660169 |
| Rights: | CC BY 4.0 |
| رقم الانضمام: | edsbas.75A85A2F |
| قاعدة البيانات: | BASE |
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