Academic Journal
Structure and evolutionary origin of the human granzyme H gene
| العنوان: | Structure and evolutionary origin of the human granzyme H gene |
|---|---|
| المؤلفون: | Haddad, P., Jenne, D., Tschopp, J., Clement, M. V., Mathieu-Mahul, D., Sasportes, M. |
| المصدر: | International Immunology, vol. 3, no. 1, pp. 57-66 |
| سنة النشر: | 1991 |
| المجموعة: | Université de Lausanne (UNIL): Serval - Serveur académique lausannois |
| مصطلحات موضوعية: | Amino Acid Sequence Base Sequence Cathepsins/genetics DNA/genetics Evolution Gene Expression Granzymes Humans Lymphocytes/enzymology Molecular Sequence Data Restriction Mapping Sequence Homology, Nucleic Acid Serine Endopeptidases/*genetics |
| الوصف: | Among the molecules proposed to be involved in cytotoxic T lymphocyte (CTL), natural killer (NK) and lymphokine activated killer (LAK) cell-mediated lysis are the granzymes, a family of serine proteases stored in the cytoplasmic granules of CTLs, NK and LAK cells. In addition to the granzymes A and B, a third member of this family has been cloned in man and designated granzyme H. We present the complete gene sequence including the 5' promoter region and demonstrate that the granzyme H sequence represents a functional gene expressed in activated T cells. Granzyme H shows the highest degree (greater than 54%) of amino acid sequence homology with granzyme B and cathepsin G and, like these genes, consists of five exons separated by introns at equivalent positions. The evolutionary history of granzyme H has been analyzed by reconstructing an evolutionary tree for granzyme sequences. We provide evidence that interlocus recombination between the ancestral genes of granzyme B and granzyme H occurred about 21 million years ago, leading to a replacement of exon 3, intron 3 and part of exon 4 in human granzyme H by human granzyme B sequences. Our results suggest that the ancestral gene of granzyme H is more closely related to cathepsin G and granzyme B than to the murine granzymes C to G. Thus, granzyme H does not represent a human counterpart of the known murine granzymes A to G. It diverged from cathepsin G before mammalian radiation and should, therefore, exist in other mammalian lineages as well. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/2049336; info:eu-repo/semantics/altIdentifier/pissn/0953-8178; info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_DC4FFD80165F6; https://serval.unil.ch/notice/serval:BIB_DC4FFD80165F; https://serval.unil.ch/resource/serval:BIB_DC4FFD80165F.P001/REF.pdf |
| DOI: | 10.1093/intimm/3.1.57 |
| الاتاحة: | https://serval.unil.ch/notice/serval:BIB_DC4FFD80165F https://doi.org/10.1093/intimm/3.1.57 https://serval.unil.ch/resource/serval:BIB_DC4FFD80165F.P001/REF.pdf http://nbn-resolving.org/urn/resolver.pl?urn=urn:nbn:ch:serval-BIB_DC4FFD80165F6 |
| Rights: | info:eu-repo/semantics/openAccess ; Copying allowed only for non-profit organizations ; https://serval.unil.ch/disclaimer |
| رقم الانضمام: | edsbas.729FFC2F |
| قاعدة البيانات: | BASE |
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