Academic Journal
Efficient induction of pluripotent stem cells differentiated into mesenchymal stem cell lineages
| العنوان: | Efficient induction of pluripotent stem cells differentiated into mesenchymal stem cell lineages |
|---|---|
| المؤلفون: | Tseng, Huan-Chin, Hsu, Teh-Fu, Lin, Yi-Ying, Lai, Wei-Yi, Liu, Yu-Hao, Yang, Yi-Ping, Chen, Cheng-Fong, Wang, Chien-Ying |
| المصدر: | Journal of the Chinese Medical Association ; ISSN 1726-4901 |
| بيانات النشر: | Ovid Technologies (Wolters Kluwer Health) |
| سنة النشر: | 2024 |
| الوصف: | Background: Mesenchymal stem cells (MSCs) have garnered significant attention in the field of cell-based therapy owing to their remarkable capabilities for differentiation and self-renewal. However, primary tissue-derived MSCs are plagued by various limitations, including constrained tissue sources, arduous and invasive retrieval procedures, heterogeneous cell populations, diminished purity, cellular senescence, and a decline in self-renewal and proliferative capacities after extended expansion. Addressing these challenges, our study focuses on establishing a robust differentiation platform to generate mesenchymal stem cells derived from induced pluripotent stem cells (iMSCs). Methods: To achieve this, we employed a comprehensive methodology involving the differentiation of induced pluripotent stem cells into mesenchymal stem cells. The process was meticulously designed to ensure the expression of key MSC positive markers (CD73, CD90, and CD105) at elevated levels, coupled with the minimal expression of negative markers (CD34, CD45, CD11b, CD19, and HLA-DR). Moreover, the stability of these characteristics was evaluated across ten generations. Result: Our findings attest to the success of this endeavor. iMSCs exhibited robust expression of positive markers and limited expression of negative markers, confirming their mesenchymal stem cell identity. Importantly, these characteristics remained stable even up to the 10th generation, signifying the potential for sustained use in therapeutic applications. Furthermore, our study demonstrated the successful differentiation of iMSCs into osteocytes, chondrocytes, and adipocytes, showcasing their multi-lineage potential. Conclusion: In conclusion, the establishment of induced pluripotent stem cell-derived mesenchymal stem cells (iMSCs) presents a significant advancement in overcoming the limitations associated with primary tissue-derived MSCs. The remarkable stability and multi-lineage differentiation potential exhibited by iMSCs offer a strong foundation for their ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1097/jcma.0000000000001058 |
| DOI: | 10.1097/JCMA.0000000000001058 |
| الاتاحة: | http://dx.doi.org/10.1097/jcma.0000000000001058 https://journals.lww.com/10.1097/JCMA.0000000000001058 |
| Rights: | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| رقم الانضمام: | edsbas.5ED5EBE5 |
| قاعدة البيانات: | BASE |
| ResultId |
1 |
|---|---|
| Header |
edsbas BASE edsbas.5ED5EBE5 995 3 Academic Journal academicJournal 995.377258300781 |
| PLink |
https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edsbas&AN=edsbas.5ED5EBE5&custid=s6537998&authtype=sso |
| FullText |
Array
(
[Availability] => 0
)
Array ( [0] => Array ( [Url] => http://dx.doi.org/10.1097/jcma.0000000000001058# [Name] => EDS - BASE [Category] => fullText [Text] => View record in BASE [MouseOverText] => View record in BASE ) ) |
| Items |
Array
(
[Name] => Title
[Label] => Title
[Group] => Ti
[Data] => Efficient induction of pluripotent stem cells differentiated into mesenchymal stem cell lineages
)
Array ( [Name] => Author [Label] => Authors [Group] => Au [Data] => <searchLink fieldCode="AR" term="%22Tseng%2C+Huan-Chin%22">Tseng, Huan-Chin</searchLink><br /><searchLink fieldCode="AR" term="%22Hsu%2C+Teh-Fu%22">Hsu, Teh-Fu</searchLink><br /><searchLink fieldCode="AR" term="%22Lin%2C+Yi-Ying%22">Lin, Yi-Ying</searchLink><br /><searchLink fieldCode="AR" term="%22Lai%2C+Wei-Yi%22">Lai, Wei-Yi</searchLink><br /><searchLink fieldCode="AR" term="%22Liu%2C+Yu-Hao%22">Liu, Yu-Hao</searchLink><br /><searchLink fieldCode="AR" term="%22Yang%2C+Yi-Ping%22">Yang, Yi-Ping</searchLink><br /><searchLink fieldCode="AR" term="%22Chen%2C+Cheng-Fong%22">Chen, Cheng-Fong</searchLink><br /><searchLink fieldCode="AR" term="%22Wang%2C+Chien-Ying%22">Wang, Chien-Ying</searchLink> ) Array ( [Name] => TitleSource [Label] => Source [Group] => Src [Data] => Journal of the Chinese Medical Association ; ISSN 1726-4901 ) Array ( [Name] => Publisher [Label] => Publisher Information [Group] => PubInfo [Data] => Ovid Technologies (Wolters Kluwer Health) ) Array ( [Name] => DatePubCY [Label] => Publication Year [Group] => Date [Data] => 2024 ) Array ( [Name] => Abstract [Label] => Description [Group] => Ab [Data] => Background: Mesenchymal stem cells (MSCs) have garnered significant attention in the field of cell-based therapy owing to their remarkable capabilities for differentiation and self-renewal. However, primary tissue-derived MSCs are plagued by various limitations, including constrained tissue sources, arduous and invasive retrieval procedures, heterogeneous cell populations, diminished purity, cellular senescence, and a decline in self-renewal and proliferative capacities after extended expansion. Addressing these challenges, our study focuses on establishing a robust differentiation platform to generate mesenchymal stem cells derived from induced pluripotent stem cells (iMSCs). Methods: To achieve this, we employed a comprehensive methodology involving the differentiation of induced pluripotent stem cells into mesenchymal stem cells. The process was meticulously designed to ensure the expression of key MSC positive markers (CD73, CD90, and CD105) at elevated levels, coupled with the minimal expression of negative markers (CD34, CD45, CD11b, CD19, and HLA-DR). Moreover, the stability of these characteristics was evaluated across ten generations. Result: Our findings attest to the success of this endeavor. iMSCs exhibited robust expression of positive markers and limited expression of negative markers, confirming their mesenchymal stem cell identity. Importantly, these characteristics remained stable even up to the 10th generation, signifying the potential for sustained use in therapeutic applications. Furthermore, our study demonstrated the successful differentiation of iMSCs into osteocytes, chondrocytes, and adipocytes, showcasing their multi-lineage potential. Conclusion: In conclusion, the establishment of induced pluripotent stem cell-derived mesenchymal stem cells (iMSCs) presents a significant advancement in overcoming the limitations associated with primary tissue-derived MSCs. The remarkable stability and multi-lineage differentiation potential exhibited by iMSCs offer a strong foundation for their ... ) Array ( [Name] => TypeDocument [Label] => Document Type [Group] => TypDoc [Data] => article in journal/newspaper ) Array ( [Name] => Language [Label] => Language [Group] => Lang [Data] => English ) Array ( [Name] => DOI [Label] => DOI [Group] => ID [Data] => 10.1097/jcma.0000000000001058 ) Array ( [Name] => DOI [Label] => DOI [Group] => ID [Data] => 10.1097/JCMA.0000000000001058 ) Array ( [Name] => URL [Label] => Availability [Group] => URL [Data] => http://dx.doi.org/10.1097/jcma.0000000000001058<br />https://journals.lww.com/10.1097/JCMA.0000000000001058 ) Array ( [Name] => Copyright [Label] => Rights [Group] => Cpyrght [Data] => http://creativecommons.org/licenses/by-nc-nd/4.0/ ) Array ( [Name] => AN [Label] => Accession Number [Group] => ID [Data] => edsbas.5ED5EBE5 ) |
| RecordInfo |
Array
(
[BibEntity] => Array
(
[Identifiers] => Array
(
[0] => Array
(
[Type] => doi
[Value] => 10.1097/jcma.0000000000001058
)
)
[Languages] => Array
(
[0] => Array
(
[Text] => English
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => Efficient induction of pluripotent stem cells differentiated into mesenchymal stem cell lineages
[Type] => main
)
)
)
[BibRelationships] => Array
(
[HasContributorRelationships] => Array
(
[0] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Tseng, Huan-Chin
)
)
)
[1] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Hsu, Teh-Fu
)
)
)
[2] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Lin, Yi-Ying
)
)
)
[3] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Lai, Wei-Yi
)
)
)
[4] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Liu, Yu-Hao
)
)
)
[5] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Yang, Yi-Ping
)
)
)
[6] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Chen, Cheng-Fong
)
)
)
[7] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Wang, Chien-Ying
)
)
)
)
[IsPartOfRelationships] => Array
(
[0] => Array
(
[BibEntity] => Array
(
[Dates] => Array
(
[0] => Array
(
[D] => 01
[M] => 01
[Type] => published
[Y] => 2024
)
)
[Identifiers] => Array
(
[0] => Array
(
[Type] => issn-locals
[Value] => edsbas
)
[1] => Array
(
[Type] => issn-locals
[Value] => edsbas.oa
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => Journal of the Chinese Medical Association ; ISSN 1726-4901
[Type] => main
)
)
)
)
)
)
)
|
| IllustrationInfo |