Academic Journal
Tri-mannose grafting of chitosan nanocarriers remodels the macrophage response to bacterial infection
| العنوان: | Tri-mannose grafting of chitosan nanocarriers remodels the macrophage response to bacterial infection |
|---|---|
| المؤلفون: | Coya, Juan Manuel, Matteis, Laura de, Giraud-Gatineau, Alexandre, Biton, Anne, Serrano-Sevilla, Inés, Danckaert, Anne, Dillies, Marie-Agnès, Gicquel, Brigitte, Fuente, Jesús M. de la, Tailleux, Ludovic |
| المساهمون: | European Commission, Institut Pasteur, Diputación General de Aragón, Ministerio de Economía y Competitividad (España) |
| بيانات النشر: | BioMed Central |
| سنة النشر: | 2019 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| مصطلحات موضوعية: | Chitosan nanocarriers, Surface grafting, Macrophages, Mycobacterium tuberculosis, Host response |
| الوصف: | [Background]: Infectious diseases are still a leading cause of death and, with the emergence of drug resistance, pose a great threat to human health. New drugs and strategies are thus urgently needed to improve treatment efficacy and limit drug-associated side effects. Nanotechnology-based drug delivery systems are promising approaches, offering hope in the fight against drug resistant bacteria. However, how nanocarriers influence the response of innate immune cells to bacterial infection is mostly unknown. ; [Results]: Here, we used Mycobacterium tuberculosis as a model of bacterial infection to examine the impact of mannose functionalization of chitosan nanocarriers (CS-NCs) on the human macrophage response. Both ungrafted and grafted CS-NCs were similarly internalized by macrophages, via an actin cytoskeleton-dependent process. Although tri-mannose ligands did not modify the capacity of CS-NCs to escape lysosomal degradation, they profoundly remodeled the response of M. tuberculosis-infected macrophages. mRNA sequencing showed nearly 900 genes to be differentially expressed due to tri-mannose grafting. Unexpectedly, the set of modulated genes was enriched for pathways involved in cell metabolism, particularly oxidative phosphorylation and sugar metabolism. ; [Conclusions]: The ability to modulate cell metabolism by grafting ligands at the surface of nanoparticles may thus be a promising strategy to reprogram immune cells and improve the efficacy of encapsulated drugs. ; This research project was co-financed by the Institut Pasteur and the European Commission as part of the European Seventh Framework Program Nanotherapeutics against Resistant Emerging Bacterial Pathogens (NAREB Project 604237), public funding from the Fondo Social de la DGA (grupos DGA), Ministerio de Economía y Competitividad del Gobierno de España for the public founding of the Proyectos I+D+I—Programa Estatal de Investigación, Desarrollo e Innovación Orientada a los Retos de la Sociedad. ; Peer reviewed |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1477-3155 |
| Relation: | #PLACEHOLDER_PARENT_METADATA_VALUE#; info:eu-repo/grantAgreement/EC/FP7/604237; Publisher's version; https://doi.org/10.1186/s12951-018-0439-x; Sí; Journal of Nanobiotechnology 17: 15 (2019); http://hdl.handle.net/10261/181680; http://dx.doi.org/10.13039/501100003329; http://dx.doi.org/10.13039/501100000780; http://dx.doi.org/10.13039/501100003762 |
| DOI: | 10.1186/s12951-018-0439-x |
| DOI: | 10.13039/501100003329 |
| DOI: | 10.13039/501100000780 |
| DOI: | 10.13039/501100003762 |
| الاتاحة: | http://hdl.handle.net/10261/181680 https://doi.org/10.1186/s12951-018-0439-x https://doi.org/10.13039/501100003329 https://doi.org/10.13039/501100000780 https://doi.org/10.13039/501100003762 |
| Rights: | open |
| رقم الانضمام: | edsbas.5CBCB565 |
| قاعدة البيانات: | BASE |
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