Academic Journal
S8. GRIN1 PROMOTER METHYLATION CHANGES IN BLOOD OF EARLY-ONSET PSYCHOTIC PATIENTS AND UNAFFECTED SIBLINGS WITH CHILDHOOD TRAUMA
| العنوان: | S8. GRIN1 PROMOTER METHYLATION CHANGES IN BLOOD OF EARLY-ONSET PSYCHOTIC PATIENTS AND UNAFFECTED SIBLINGS WITH CHILDHOOD TRAUMA |
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| المؤلفون: | Loureiro, Camila, Fabiana, Corsi-Zuelli, Helene Aparecida, Fachim, Rosana, Shuhama, Paulo Rossi, Menezes, Caroline, Dalton F, Cristina Marta, Del-Ben, Reynolds, Gavin P, Paulo, Louzada-Junior |
| المصدر: | Schizophrenia Bulletin ; volume 46, issue Supplement_1, page S32-S33 ; ISSN 0586-7614 1745-1701 |
| بيانات النشر: | Oxford University Press (OUP) |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Psychiatry and Mental health |
| الوصف: | Background Childhood trauma may lead to impairments in brain development and increases risk at psychiatric disorders. Evidence also suggests that childhood trauma may affect DNA methylation patterns consequently influencing gene expression (Tomassi et al., 2017). Some of this linking may be correlated with N-methyl-d-aspartate receptor (NMDAR) hypofunction, which plays a major role of central aspects of cognitive and negative features of schizophrenia (Lakhan et al., 2013). Specifically, the GRIN1 gene codes the biologically relevant NMDAR subunit involved in the synaptic plasticity which is expressed in a broad of non-neuronal cells (Hogan-Cann et al., 2016). Aims: We investigated DNA methylation in the promoter region of GRIN1 and LINE-1 methylation in first-episode psychosis patients (FEP), their unaffected siblings and community-based controls with and without childhood trauma. We also tested for correlations between GRIN1 methylation and NR1 concentrations in peripheral blood. Methods This study is a part of the epidemiological investigation that estimated the incidence of psychosis and the role of environmental and biological factors in psychosis aetiology in the catchment area of Ribeirão Preto, Brazil, from 1st April 2012 to 31st March 2015. The genomic DNA was extracted from blood of 60 FEP patients, 30 of their unaffected siblings and 60 age- and sex-matched community-based controls. Diagnosis and clinical characteristics were assessed using the DSM-IV (First et al., 1997; Del-Ben et al., 2001) and history of childhood trauma was assessed using the Childhood Trauma Questionnaire (Grassi-Oliverira et al., 2006). The genomic DNA was bisulfite converted and pyrosequencing was used to determine methylation levels in three CpGs sites of the GRIN1 gene and of LINE-1, as a measure of global methylation. NR1 plasma concentrations were measured using ELISA (MyBioSource, San Diego, USA). Data were analyzed using General Linear Model with post-hoc Bonferroni correction and Pearson’s correlations. Results ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1093/schbul/sbaa031.074 |
| الاتاحة: | http://dx.doi.org/10.1093/schbul/sbaa031.074 http://academic.oup.com/schizophreniabulletin/article-pdf/46/Supplement_1/S32/33288707/sbaa031.074.pdf |
| Rights: | http://creativecommons.org/licenses/by-nc/4.0/ |
| رقم الانضمام: | edsbas.52837237 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/schbul/sbaa031.074 |
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