Academic Journal
The Off-rate of Monomers Dissociating from Amyloid-ß Protofibrils
| العنوان: | The Off-rate of Monomers Dissociating from Amyloid-ß Protofibrils |
|---|---|
| المؤلفون: | Gruning, C. S. R., Klinker, S., Wolff, M., Schneider, M., Toksöz, K., Klein, A. N., Nagel-Steger, L., Willbold, D., Hoyer, W. |
| المصدر: | The journal of biological chemistry 288(52), 37104 - 37111 (2013). doi:10.1074/jbc.M113.513432 |
| بيانات النشر: | Soc. |
| سنة النشر: | 2013 |
| المجموعة: | Forschungszentrum Jülich: JuSER (Juelich Shared Electronic Resources) |
| مصطلحات موضوعية: | info:eu-repo/classification/ddc/570 |
| جغرافية الموضوع: | DE |
| الوصف: | The interconversion of monomers, oligomers, and amyloid fibrils of the amyloid-peptide (A) has been implicated in the pathogenesis of Alzheimer disease. The determination of the kinetics of the individual association and dissociation reactions is hampered by the fact that forward and reverse reactions to/from different aggregation states occur simultaneously. Here, we report the kinetics of dissociation of A Monomers from protofibrils, prefibrillar high molecular weight oligomers previously shown to possess pronounced neurotoxicity. An engineered binding protein sequestering specifically mono-meric A was employed to follow protofibril dissociation by tryptophan fluorescence, precluding confounding effects of reverse or competing reactions. A protofibril dissociation into monomers follows exponential decay kinetics with a time constant of ~2 h at 25 °C and an activation energy of 80 kJ/mol, values typical for high affinity biomolecular interactions. This study demonstrates the high kinetic stability of A protofibrils toward dissociation into monomers and supports the delineation of the A folding and assembly energy landscape. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/issn/0021-9258; info:eu-repo/semantics/altIdentifier/wos/WOS:000329189700030; info:eu-repo/semantics/altIdentifier/issn/1083-351X; https://juser.fz-juelich.de/record/141924; https://juser.fz-juelich.de/search?p=id:%22FZJ-2014-00261%22 |
| الاتاحة: | https://juser.fz-juelich.de/record/141924 https://juser.fz-juelich.de/search?p=id:%22FZJ-2014-00261%22 |
| Rights: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.50940F1 |
| قاعدة البيانات: | BASE |
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