Academic Journal

Magnesium–Isotope Fractionation in Chlorophyll-a Extracted from Two Plants with Different Pathways of Carbon Fixation (C3, C4)

التفاصيل البيبلوغرافية
العنوان: Magnesium–Isotope Fractionation in Chlorophyll-a Extracted from Two Plants with Different Pathways of Carbon Fixation (C3, C4)
المؤلفون: Katarzyna Wrobel, Jakub Karasiński, Andrii Tupys, Missael Antonio Arroyo Negrete, Ludwik Halicz, Kazimierz Wrobel, Ewa Bulska
المصدر: Molecules; Volume 25; Issue 7; Pages: 1644
بيانات النشر: Multidisciplinary Digital Publishing Institute
سنة النشر: 2020
المجموعة: MDPI Open Access Publishing
مصطلحات موضوعية: Mg-isotope fractionation, chlorophyll, C3 plants, C4 plants, ion chromatography, multiple collector-inductively coupled mass spectrometry
جغرافية الموضوع: agris
الوصف: Relatively few studies have been focused so far on magnesium–isotope fractionation during plant growth, element uptake from soil, root-to-leaves transport and during chlorophylls biosynthesis. In this work, maize and garden cress were hydroponically grown in identical conditions in order to examine if the carbon fixation pathway (C4, C3, respectively) might have impact on Mg-isotope fractionation in chlorophyll-a. The pigment was purified from plants extracts by preparative reversed phase chromatography, and its identity was confirmed by high-resolution mass spectrometry. The green parts of plants and chlorophyll-a fractions were acid-digested and submitted to ion chromatography coupled through desolvation system to multiple collector inductively coupled plasma-mass spectrometry. Clear preference for heavy Mg-isotopes was found in maize green parts (∆26Mgplant-nutrient 0.65, 0.74 for two biological replicates, respectively) and in chlorophyll-a (∆26Mgchlorophyll-plant 1.51, 2.19). In garden cress, heavy isotopes were depleted in green parts (∆26Mgplant-nutrient (−0.87)–(−0.92)) and the preference for heavy isotopes in chlorophyll-a was less marked relative to maize (∆26Mgchlorophyll-plant 0.55–0.52). The observed effect might be ascribed to overall higher production of energy in form of adenosine triphosphate (ATP), required for carbon fixation in C4 compared to C3, which could reduce kinetic barrier and make equilibrium fractionation prevailing during magnesium incorporation to protoporphyrin ring.
نوع الوثيقة: text
وصف الملف: application/pdf
اللغة: English
Relation: Analytical Chemistry; https://dx.doi.org/10.3390/molecules25071644
DOI: 10.3390/molecules25071644
الاتاحة: https://doi.org/10.3390/molecules25071644
Rights: https://creativecommons.org/licenses/by/4.0/
رقم الانضمام: edsbas.4CD4E3A4
قاعدة البيانات: BASE
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