Academic Journal
Impact of autoclave sterilization on the activity and structure of formulated heparin
| العنوان: | Impact of autoclave sterilization on the activity and structure of formulated heparin |
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| المؤلفون: | Beaudet, Julie M., Weyers, Amanda, Solakyildirim, Kemal, Yang, Bo, Takieddin, Majde, Mousa, Shaker, Zhang, Fuming, Linhardt, Robert J. |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Biology, Chemistry and chemical biology, Chemical and biological engineering, Biomedical engineering |
| الوصف: | Journal of Pharmaceutical Science, 100, 3396–3404 ; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform. ; The stability of a formulated heparin was examined during its sterilization by autoclaving. A new method to follow loss in heparin binding to the serine protease inhibitor, antithrombin III, and the serine protease, thrombin, was developed using a surface plasmon resonance competitive binding assay. This loss in binding affinity correlated well with loss in antifactor IIa (thrombin) activity as well as antifactor Xa activity as measured using conventional amidolytic assays. Autoclaving also resulted in a modest breakdown of the heparin backbone as confirmed by a slight reduction in number-averaged and weight-averaged molecular weight and an increase in polydispersity. Although no clear changes were observed by nuclear magnetic resonance spectroscopy, disaccharide composition analysis using high-performance liquid chromatography-electrospray ionization-mass spectrometry suggested that loss of selected sulfo groups had taken place. It is this sulfo group loss that probably accounts for a decrease in the binding of autoclaved heparin to antithrombin III and thrombin as well as the observed decrease in its amidolytic activity. ; National Institutes of Health |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 15206017 |
| Relation: | The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS); The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Journal of Pharmaceutical Sciences; https://harc.rpi.edu/; Impact of autoclave sterilization on the activity and structure of formulated heparin, J. M. Beaudet, A. Weyers, K. Solakyildirim, B. Yang, M. Takieddin, S. Mousa, F. Zhang, R. J. Linhardt, Journal of Pharmaceutical Science, 100, 3396–3404, 2011.; 223549; https://hdl.handle.net/20.500.13015/5287; https://doi.org/10.1002/jps.22527 |
| DOI: | 10.1002/jps.22527 |
| الاتاحة: | https://hdl.handle.net/20.500.13015/5287 https://doi.org/10.1002/jps.22527 |
| Rights: | In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/ |
| رقم الانضمام: | edsbas.4B409 |
| قاعدة البيانات: | BASE |
| تدمد: | 15206017 |
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| DOI: | 10.1002/jps.22527 |