Dissertation/ Thesis
Acoustic based technologies combined with enzymatic amplification for the analysis of point mutations in Tissue and Liquid biopsy ; Τεχνολογίες ακουστικών βιοαισθητήρων σε συνδυασμό με ενζυμικό πολλαπλασιασμό για την ανίχνευση σημειακών μεταλλαγών στη βιοψία ιστού και στην υγρή βιοψία
| العنوان: | Acoustic based technologies combined with enzymatic amplification for the analysis of point mutations in Tissue and Liquid biopsy ; Τεχνολογίες ακουστικών βιοαισθητήρων σε συνδυασμό με ενζυμικό πολλαπλασιασμό για την ανίχνευση σημειακών μεταλλαγών στη βιοψία ιστού και στην υγρή βιοψία |
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| المؤلفون: | Naoumi, Nikoletta, Ναούμη, Νικολέττα |
| بيانات النشر: | University of Crete (UOC) Πανεπιστήμιο Κρήτης |
| سنة النشر: | 2021 |
| المجموعة: | National Archive of PhD Theses (National Documentation Centre Greece) |
| مصطلحات موضوعية: | Ακουστικοί βιοαισθητήρες, Υψηλής θεμελιώδους συχνότητας QCM, Αλληλοειδική αλυσηδωτή αντίδραση πολυμεράσης, Αλυσηδωτή αντίδραση λιγάσης, Ακουστικός πολλαπλασιασμός μέσω λιποσωμάτων, Μοριακά διαγνωστικά, Κλινική ογκολογία, Acoustic biosensors, High fundamental frequency QCM, Allele-specific PCR, Ligase Chain Reaction, Liposomes acoustic amplification, Molecular diagnostics, Clinical oncology, Βιολογία, Φυσικές Επιστήμες, άλλοι τομείς, Biological Sciences, Natural Sciences, Biology, miscellaneous |
| الوصف: | Although biosensors hold their roots in the early nineteen-sixties, a great increase in the number of publications refer on this topic was recorded after the nineteen-eighties decade. Moreover, the increasing demand for inexpensive, simplified, sensitive and reliable sensors lead the biosensors market to a significant growth with a predicted compound annual growth rate (CAGR) of 7.3% for the years 2020 - 2027. Biosensors are applied in a wide range of fields including, healthcare and medical diagnostics and is expected to be positively affected by the new trend of precision medicine and the evenly huge market of Liquid biopsy.Regular screening of cancerous point mutations is of great importance for efficient cancer management and treatment selection. Although excellent techniques like next-generation sequencing and the ultrasensitive droplet digital PCR have been developed, these techniques are lacking in fastness, simplicity and cost-effectiveness. The work presented here focuses on the development of new diagnostic approaches for the detection of ultralow concentrations of cancerous point mutations in human DNA utilizing acoustic biosensors combined with molecular amplification assays. Firstly, we present a universal acoustic methodology that involves the direct immobilization of biotinylated DNA targets on the sensor surface followed by liposome-based acoustic detection. Liposomes, are large nanoparticles (here 200nm) acting as acoustic energy-dissipation signal enhancers. For the DNA immobilization, we developed a surface chemistry composed of biotinylated-BSA and NAv; the substrate was reproducible and was successfully validated for its specificity and stability upon liposome and crude sample additions, respectively. While for the above we used a standard QCM-D device and a 5 MHz QCM sensor, a novel High Fundamental Frequency QCM (HFF-QCM) array of 24 miniaturized sensors operating at 150 MHz and a new acoustic device were also tested. The array permitted the faster and more cost-effective analysis of up to ... |
| نوع الوثيقة: | doctoral or postdoctoral thesis |
| اللغة: | English |
| Relation: | http://hdl.handle.net/10442/hedi/51078 |
| DOI: | 10.12681/eadd/51078 |
| الاتاحة: | http://hdl.handle.net/10442/hedi/51078 https://doi.org/10.12681/eadd/51078 |
| رقم الانضمام: | edsbas.49104CA6 |
| قاعدة البيانات: | BASE |
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