Academic Journal

Sample preparation and culture conditions effects on MALDI-TOF MS identification of bacteria: A review

التفاصيل البيبلوغرافية
العنوان: Sample preparation and culture conditions effects on MALDI-TOF MS identification of bacteria: A review
المؤلفون: Topić Popović, Natalija, Kazazić, Snježana, Bojanić, Krunoslav, Strunjak-Perović, Ivančica, Čož-Rakovac, Rozelindra
المصدر: Mass spectrometry reviews
بيانات النشر: Wiley
سنة النشر: 2023
المجموعة: Ruđer Bošković Institute Zagreb: Full-text Institutional Repository (FULIR)
مصطلحات موضوعية: Biology, Veterinary Medicine, Biotechnology
الوصف: Matrix-assisted laser desorption/ionization time- of-flight mass spectrometry(MALDI-TOF MS) is an excellent tool for bacterial identification. It allows high throughput, sensitive and specific applications in clinical diagnostics and environ- mental research. Currently, there is no optimal standardized protocol for sample preparation and culture conditions to profile bacteria. The performance of MALDITOF MS is affected by several variables, such as sample preparation, culture media and culture conditions, incubation time/growth stage, incubation temperature, high salt content, blood in the culture media, and others. This review thus aims to clarify why a uniformed protocol is not plausible, to assess the effects these factors have onMALDITOF MS identification score, and discuss possible optimizations for its methodology, in relation to specific bacterial representatives and strain requirements.
نوع الوثيقة: article in journal/newspaper
وصف الملف: application/pdf
اللغة: English
Relation: info:eu-repo/grantAgreement/MZOS/ZP/N/A; http://fulir.irb.hr/8178/; https://doi.org/10.1002/mas.21739; http://fulir.irb.hr/8178/1/Mass%20Spec%20Rev_Maldi.pdf
DOI: 10.1002/mas.21739
الاتاحة: http://fulir.irb.hr/8178/
http://fulir.irb.hr/8178/1/Mass%20Spec%20Rev_Maldi.pdf
https://doi.org/10.1002/mas.21739
Rights: info:eu-repo/semantics/openAccess
رقم الانضمام: edsbas.4571ED62
قاعدة البيانات: BASE
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