Academic Journal
Mesenchymal Stem Cells Resist Mechanical Confinement through the Activation of the Cortex during Cell Division
| العنوان: | Mesenchymal Stem Cells Resist Mechanical Confinement through the Activation of the Cortex during Cell Division |
|---|---|
| المؤلفون: | Chuanchuan Lin (8485128), Ye He (391485), Kun Xu (116877), Qian Feng (302178), Xuan Li (137217), Songyue Zhang (7509764), Ke Li (106849), Ruqing Bai (11247836), Hong Jiang (79543), Kaiyong Cai (683228) |
| سنة النشر: | 1753 |
| المجموعة: | Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: | Biophysics, Medicine, Cell Biology, Genetics, Physiology, Biotechnology, Developmental Biology, Cancer, Computational Biology, Space Science, Biological Sciences not elsewhere classified, RAS, matrix stiffness, MSC, Mesenchymal Stem Cells, II, cell culture system, ECM |
| الوصف: | The mechanical properties of the natural extracellular matrix (ECM) change extensively, but these specific properties provide a relatively stable environment for resident cells. Although the effect of matrix stiffness on cell functions has been widely studied, the molecular mechanism was still not fully understood. Matrix stiffening is a common phenomenon in tissue damaging processes. To explore the effect of the increase in local matrix stiffness on cell behaviors, a three-dimensional (3D) cell culture system with a tunable modulus but constant other physical parameters was constructed by the alginate hydrogel with different molecular weights and cross-linking degrees. By using this culture system, the transcriptome response of mesenchymal stem cells (MSCs) to matrix stiffness was explored. Furthermore, a finite element model was developed to simulate the interaction between cells and the matrix. Results revealed that the increased matrix stiffness promoted the proliferation-related signaling of MSCs, and this process depended on the increased cortex tension caused by the activation of RAS and myosin II. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| Relation: | https://figshare.com/articles/journal_contribution/Mesenchymal_Stem_Cells_Resist_Mechanical_Confinement_through_the_Activation_of_the_Cortex_during_Cell_Division/15133040 |
| DOI: | 10.1021/acsbiomaterials.1c00862.s001 |
| الاتاحة: | https://doi.org/10.1021/acsbiomaterials.1c00862.s001 |
| Rights: | CC BY-NC 4.0 |
| رقم الانضمام: | edsbas.40D6E7E |
| قاعدة البيانات: | BASE |
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