Academic Journal
PEG35 as a Preconditioning Agent against Hypoxia/Reoxygenation Injury
| العنوان: | PEG35 as a Preconditioning Agent against Hypoxia/Reoxygenation Injury |
|---|---|
| المؤلفون: | Silva, Rui Teixeira da, Machado, Ivo F., Teodoro, João S., Panisello-Roselló, Arnau, Roselló-Catafau, Joan, Rolo, Anabela P., Palmeira, Carlos M. |
| سنة النشر: | 2022 |
| المجموعة: | Universidade de Coimbra: Estudo Geral |
| مصطلحات موضوعية: | polyethylene glycol 35, hypoxia/reoxygenation injury, mitochondria, autophagy, Humans, Hypoxia, Ischemic Preconditioning, Liver, Membrane Potential, Mitochondrial, Polyethylene Glycols, Protective Agents, Reperfusion Injury |
| الوصف: | Pharmacological conditioning is a protective strategy against ischemia/reperfusion injury, which occurs during liver resection and transplantation. Polyethylene glycols have shown multiple benefits in cell and organ preservation, including antioxidant capacity, edema prevention and membrane stabilization. Recently, polyethylene glycol 35 kDa (PEG35) preconditioning resulted in decreased hepatic injury and protected the mitochondria in a rat model of cold ischemia. Thus, the study aimed to decipher the mechanisms underlying PEG35 preconditioning-induced protection against ischemia/reperfusion injury. A hypoxia/reoxygenation model using HepG2 cells was established to evaluate the effects of PEG35 preconditioning. Several parameters were assessed, including cell viability, mitochondrial membrane potential, ROS production, ATP levels, protein content and gene expression to investigate autophagy, mitochondrial biogenesis and dynamics. PEG35 preconditioning preserved the mitochondrial function by decreasing the excessive production of ROS and subsequent ATP depletion, as well as by recovering the membrane potential. Furthermore, PEG35 increased levels of autophagy-related proteins and the expression of genes involved in mitochondrial biogenesis and fusion. In conclusion, PEG35 preconditioning effectively ameliorates hepatic hypoxia/reoxygenation injury through the enhancement of autophagy and mitochondrial quality control. Therefore, PEG35 could be useful as a potential pharmacological tool for attenuating hepatic ischemia/reperfusion injury in clinical practice. ; This research was funded by the European Union’s Horizon 2020 Research and Innovation Programme under the Marie Skłodowska-Curie Grant Agreement No. 722619 (FOIE GRAS). R.T.d.S. was a recipient of a FOIE GRAS Early Research Training Grant (Agreement No. 722619). The research was also financed by the European Regional Development Fund (ERDF), through the Centro 2020 Regional Operational Program: project CENTRO-01-0145-FEDER-000012-HealthyAging2020, the ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1422-0067 |
| Relation: | http://hdl.handle.net/10316/103330 |
| DOI: | 10.3390/ijms23031156 |
| الاتاحة: | http://hdl.handle.net/10316/103330 https://doi.org/10.3390/ijms23031156 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.395E03C2 |
| قاعدة البيانات: | BASE |
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