Academic Journal
Ultrastructural Changes of Blood Cells in Children with Generalized Purulent Peritonitis: A Cross-Sectional and Prospective Study
| العنوان: | Ultrastructural Changes of Blood Cells in Children with Generalized Purulent Peritonitis: A Cross-Sectional and Prospective Study |
|---|---|
| المؤلفون: | Ulyana Halyuk, Olena Lychkovska, Oksana Mota, Vasyl Kovalyshyn, Natalia Kech, Petro Pokotylo, Olena Trutiak, Bożena Zboina, Grzegorz Józef Nowicki, Barbara Ślusarska |
| المصدر: | Children; Volume 7; Issue 10; Pages: 189 |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute |
| سنة النشر: | 2020 |
| المجموعة: | MDPI Open Access Publishing |
| مصطلحات موضوعية: | purulent peritonitis, acute appendicitis, blood cells, ultrastructural changes |
| الوصف: | In conditions of abdominal sepsis with indications of first- or second-stage shock, blood cells undergo significant ultrastructural changes that cause impaired gas exchange, changes in reactivity, and decompensation of organs and systems functions. This paper presents a cross-sectional prospective study aimed at researching the ultrastructure of blood cells in children experiencing abdominal septic shock against the background of generalized purulent peritonitis of appendicular origin. This study was conducted with 15 children aged 6–12 who were undergoing treatment for generalized appendicular purulent peritonitis, with first- or second-stage abdominal septic shock, in emergency care. The changes in the ultrastructure of erythrocytes did not correspond to changes characteristic of eryptosis, which confirms their occurrence under the influence of such pathogenic factors as intoxication, metabolic, water–electrolyte balance, and acid–base disorders. Ultrastructural changes of granulocytes indicate their hyperactivation, which leads to the exhaustion of membrane synthetic resources, membrane destruction, ineffective expenditure of bactericidal factors on substrates that are not subject to destruction. In lymphocytes, disorganization of the nuclear membrane and intracellular membranes, uneven distribution of chromatin, the hypertrophied Golgi apparatus, and a large number of young mitochondria, lysosomes, ribosomes, vesicles manifesting the disruption of metabolism, stress and decompensation of energy supply and protein synthesis systems, have been demonstrated. In conditions of abdominal sepsis with indications of first- or second-stage shock, blood cells undergo substantial ultrastructural changes causing gas exchange disruption, changes in reactivity, as well as decompensation of organs and system functioning. |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://dx.doi.org/10.3390/children7100189 |
| DOI: | 10.3390/children7100189 |
| الاتاحة: | https://doi.org/10.3390/children7100189 |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.366D025F |
| قاعدة البيانات: | BASE |
| ResultId |
1 |
|---|---|
| Header |
edsbas BASE edsbas.366D025F 905 3 Academic Journal academicJournal 905.017944335938 |
| PLink |
https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edsbas&AN=edsbas.366D025F&custid=s6537998&authtype=sso |
| FullText |
Array
(
[Availability] => 0
)
Array ( [0] => Array ( [Url] => https://doi.org/10.3390/children7100189# [Name] => EDS - BASE [Category] => fullText [Text] => View record in BASE [MouseOverText] => View record in BASE ) ) |
| Items |
Array
(
[Name] => Title
[Label] => Title
[Group] => Ti
[Data] => Ultrastructural Changes of Blood Cells in Children with Generalized Purulent Peritonitis: A Cross-Sectional and Prospective Study
)
Array ( [Name] => Author [Label] => Authors [Group] => Au [Data] => <searchLink fieldCode="AR" term="%22Ulyana+Halyuk%22">Ulyana Halyuk</searchLink><br /><searchLink fieldCode="AR" term="%22Olena+Lychkovska%22">Olena Lychkovska</searchLink><br /><searchLink fieldCode="AR" term="%22Oksana+Mota%22">Oksana Mota</searchLink><br /><searchLink fieldCode="AR" term="%22Vasyl+Kovalyshyn%22">Vasyl Kovalyshyn</searchLink><br /><searchLink fieldCode="AR" term="%22Natalia+Kech%22">Natalia Kech</searchLink><br /><searchLink fieldCode="AR" term="%22Petro+Pokotylo%22">Petro Pokotylo</searchLink><br /><searchLink fieldCode="AR" term="%22Olena+Trutiak%22">Olena Trutiak</searchLink><br /><searchLink fieldCode="AR" term="%22Bożena+Zboina%22">Bożena Zboina</searchLink><br /><searchLink fieldCode="AR" term="%22Grzegorz+Józef+Nowicki%22">Grzegorz Józef Nowicki</searchLink><br /><searchLink fieldCode="AR" term="%22Barbara+Ślusarska%22">Barbara Ślusarska</searchLink> ) Array ( [Name] => TitleSource [Label] => Source [Group] => Src [Data] => Children; Volume 7; Issue 10; Pages: 189 ) Array ( [Name] => Publisher [Label] => Publisher Information [Group] => PubInfo [Data] => Multidisciplinary Digital Publishing Institute ) Array ( [Name] => DatePubCY [Label] => Publication Year [Group] => Date [Data] => 2020 ) Array ( [Name] => Subset [Label] => Collection [Group] => HoldingsInfo [Data] => MDPI Open Access Publishing ) Array ( [Name] => Subject [Label] => Subject Terms [Group] => Su [Data] => <searchLink fieldCode="DE" term="%22purulent+peritonitis%22">purulent peritonitis</searchLink><br /><searchLink fieldCode="DE" term="%22acute+appendicitis%22">acute appendicitis</searchLink><br /><searchLink fieldCode="DE" term="%22blood+cells%22">blood cells</searchLink><br /><searchLink fieldCode="DE" term="%22ultrastructural+changes%22">ultrastructural changes</searchLink> ) Array ( [Name] => Abstract [Label] => Description [Group] => Ab [Data] => In conditions of abdominal sepsis with indications of first- or second-stage shock, blood cells undergo significant ultrastructural changes that cause impaired gas exchange, changes in reactivity, and decompensation of organs and systems functions. This paper presents a cross-sectional prospective study aimed at researching the ultrastructure of blood cells in children experiencing abdominal septic shock against the background of generalized purulent peritonitis of appendicular origin. This study was conducted with 15 children aged 6–12 who were undergoing treatment for generalized appendicular purulent peritonitis, with first- or second-stage abdominal septic shock, in emergency care. The changes in the ultrastructure of erythrocytes did not correspond to changes characteristic of eryptosis, which confirms their occurrence under the influence of such pathogenic factors as intoxication, metabolic, water–electrolyte balance, and acid–base disorders. Ultrastructural changes of granulocytes indicate their hyperactivation, which leads to the exhaustion of membrane synthetic resources, membrane destruction, ineffective expenditure of bactericidal factors on substrates that are not subject to destruction. In lymphocytes, disorganization of the nuclear membrane and intracellular membranes, uneven distribution of chromatin, the hypertrophied Golgi apparatus, and a large number of young mitochondria, lysosomes, ribosomes, vesicles manifesting the disruption of metabolism, stress and decompensation of energy supply and protein synthesis systems, have been demonstrated. In conditions of abdominal sepsis with indications of first- or second-stage shock, blood cells undergo substantial ultrastructural changes causing gas exchange disruption, changes in reactivity, as well as decompensation of organs and system functioning. ) Array ( [Name] => TypeDocument [Label] => Document Type [Group] => TypDoc [Data] => text ) Array ( [Name] => Format [Label] => File Description [Group] => SrcInfo [Data] => application/pdf ) Array ( [Name] => Language [Label] => Language [Group] => Lang [Data] => English ) Array ( [Name] => NoteTitleSource [Label] => Relation [Group] => SrcInfo [Data] => https://dx.doi.org/10.3390/children7100189 ) Array ( [Name] => DOI [Label] => DOI [Group] => ID [Data] => 10.3390/children7100189 ) Array ( [Name] => URL [Label] => Availability [Group] => URL [Data] => https://doi.org/10.3390/children7100189 ) Array ( [Name] => Copyright [Label] => Rights [Group] => Cpyrght [Data] => https://creativecommons.org/licenses/by/4.0/ ) Array ( [Name] => AN [Label] => Accession Number [Group] => ID [Data] => edsbas.366D025F ) |
| RecordInfo |
Array
(
[BibEntity] => Array
(
[Identifiers] => Array
(
[0] => Array
(
[Type] => doi
[Value] => 10.3390/children7100189
)
)
[Languages] => Array
(
[0] => Array
(
[Text] => English
)
)
[Subjects] => Array
(
[0] => Array
(
[SubjectFull] => purulent peritonitis
[Type] => general
)
[1] => Array
(
[SubjectFull] => acute appendicitis
[Type] => general
)
[2] => Array
(
[SubjectFull] => blood cells
[Type] => general
)
[3] => Array
(
[SubjectFull] => ultrastructural changes
[Type] => general
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => Ultrastructural Changes of Blood Cells in Children with Generalized Purulent Peritonitis: A Cross-Sectional and Prospective Study
[Type] => main
)
)
)
[BibRelationships] => Array
(
[HasContributorRelationships] => Array
(
[0] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Ulyana Halyuk
)
)
)
[1] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Olena Lychkovska
)
)
)
[2] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Oksana Mota
)
)
)
[3] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Vasyl Kovalyshyn
)
)
)
[4] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Natalia Kech
)
)
)
[5] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Petro Pokotylo
)
)
)
[6] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Olena Trutiak
)
)
)
[7] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Bożena Zboina
)
)
)
[8] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Grzegorz Józef Nowicki
)
)
)
[9] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Barbara Ślusarska
)
)
)
)
[IsPartOfRelationships] => Array
(
[0] => Array
(
[BibEntity] => Array
(
[Dates] => Array
(
[0] => Array
(
[D] => 01
[M] => 01
[Type] => published
[Y] => 2020
)
)
[Identifiers] => Array
(
[0] => Array
(
[Type] => issn-locals
[Value] => edsbas
)
[1] => Array
(
[Type] => issn-locals
[Value] => edsbas.oa
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => Children; Volume 7; Issue 10; Pages: 189
[Type] => main
)
)
)
)
)
)
)
|
| IllustrationInfo |