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KH176m affects spheroid growth and expression of mPGES-1 of DU145 cells.
| العنوان: | KH176m affects spheroid growth and expression of mPGES-1 of DU145 cells. |
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| المؤلفون: | Xiaolan Jiang (408276), Herma Renkema (11105122), Jan Smeitink (3455747), Julien Beyrath (4516507) |
| سنة النشر: | 2021 |
| المجموعة: | Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: | Biochemistry, Microbiology, Cell Biology, Genetics, Molecular Biology, Biotechnology, Developmental Biology, Cancer, Infectious Diseases, Plant Biology, Virology, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, DU, sonlicromanol metabolite KH 176m, cancer spheroid growth Aggressiveness, metabolite KH 176m normalizes prost., KH 176m, LNCaP cell line, exogenous PGE 2, novel treatment approach, CSC, prostaglandin E synthase, prostate cancer cells-derived spher., mPGES, expression |
| الوصف: | DU145 or LNCaP human prostate cancer cells were grown in Matrigel to induce spheroid formation and treated with vehicle or different concentrations of KH176m. (A) Representative images of spheroids grown from DU145 or LNCaP (-/+KH176m). Scale bars represent 100 microns; (B) Quantification of the average spheroid size (total area of spheroids / number of spheroids) of this experiment. Each data point corresponds to the average size of spheroids from 1 image stack. Data are means ±SD (n = 10–14, from 3 independent biological repeats). Parallel experiments were used for the analysis of mPGES-1 protein (C) or mRNA (D) expression in DU145 derived spheroids by western blot or qPCR, respectively (n = 3, for both C and D). Blk: Blank, no treatment; Veh: Vehicle (0.1% DMSO). *, p<0 . 05; **, p<0 . 005; ***, p<0 . 001 ; significant differences compared with Veh. |
| نوع الوثيقة: | still image |
| اللغة: | unknown |
| Relation: | https://figshare.com/articles/figure/KH176m_affects_spheroid_growth_and_expression_of_mPGES-1_of_DU145_cells_/14940262 |
| DOI: | 10.1371/journal.pone.0254315.g002 |
| الاتاحة: | https://doi.org/10.1371/journal.pone.0254315.g002 |
| Rights: | CC BY 4.0 |
| رقم الانضمام: | edsbas.31004399 |
| قاعدة البيانات: | BASE |
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