Academic Journal
Actin waves do not boost neurite outgrowth in the early stages of neuron maturation
| العنوان: | Actin waves do not boost neurite outgrowth in the early stages of neuron maturation |
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| المؤلفون: | Mortal Simone, Iseppon Federico, Perissinotto Andrea, D’Este Elisa, Cojoc Dan, Napolitano Luisa M. R, Torre Vincent |
| المساهمون: | Mortal, Simone, Iseppon, Federico, Perissinotto, Andrea, D’Este, Elisa, Cojoc, Dan, Napolitano Luisa, M. R., Torre, Vincent |
| سنة النشر: | 2017 |
| المجموعة: | International School for Advanced Studies (SISSA), Trieste: SISSA Digital Library (SDL) |
| مصطلحات موضوعية: | Actin waves (AWs), B-cyclodextrin, Growth cones (GCs), Myosin IIB, RhoGTPase, Cellular and Molecular Neuroscience, Settore BIO/11 - Biologia Molecolare |
| الوصف: | During neurite development, Actin Waves (AWs) emerge at the neurite base and move up to its tip, causing a transient retraction of the Growth Cone (GC). Many studies have shown that AWs are linked to outbursts of neurite growth and, therefore, contribute to the fast elongation of the nascent axon. Using long term live cell-imaging, we show that AWs do not boost neurite outgrowth and that neurites without AWs can elongate for several hundred microns. Inhibition of Myosin II abolishes the transient GC retraction and strongly modifies the AWs morphology. Super-resolution nanoscopy shows that Myosin IIB shapes the growth cone-like AWs structure and is differently distributed in AWs and GCs. Interestingly, depletion of membrane cholesterol and inhibition of Rho GTPases decrease AWs frequency and velocity. Our results indicate that Myosin IIB, membrane tension, and small Rho GTPases are important players in the regulation of the AW dynamics. Finally, we suggest a role for AWs in maintaining the GCs active during environmental exploration. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/29326552; info:eu-repo/semantics/altIdentifier/wos/WOS:000418178500001; volume:11; issue:December; journal:FRONTIERS IN CELLULAR NEUROSCIENCE; http://hdl.handle.net/20.500.11767/67880; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85040907520; http://journal.frontiersin.org/article/10.3389/fncel.2017.00402/full; https://www.frontiersin.org/articles/10.3389/fncel.2017.00402/full#supplementary-material |
| DOI: | 10.3389/fncel.2017.00402 |
| DOI: | 10.3389/fncel.2017.00402/full |
| DOI: | 10.3389/fncel.2017.00402/full#supplementary-material |
| الاتاحة: | https://hdl.handle.net/20.500.11767/67880 https://doi.org/10.3389/fncel.2017.00402 http://journal.frontiersin.org/article/10.3389/fncel.2017.00402/full https://www.frontiersin.org/articles/10.3389/fncel.2017.00402/full#supplementary-material |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.30D03C9A |
| قاعدة البيانات: | BASE |
| DOI: | 10.3389/fncel.2017.00402 |
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