Academic Journal
A novel bivalent Pasteurellosis-RHD vaccine candidate adjuvanted with Montanide ISA70 protects rabbits from lethal challenge
| العنوان: | A novel bivalent Pasteurellosis-RHD vaccine candidate adjuvanted with Montanide ISA70 protects rabbits from lethal challenge |
|---|---|
| المؤلفون: | Jakeen K. El-Jakee, Ihab M. Moussa, Mai S. Omran, Basem M. Ahmed, Mahmoud A. Elgamal, Hassan A. Hemeg, Ayman S. Mubarak, Khalid S. Al-Maary, Saleh A. Kabli, Sherif A. Marouf, Jwaher Haji Alhaaji |
| المصدر: | Saudi Journal of Biological Sciences, Vol 27, Iss 3, Pp 996-1001 (2020) |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2020 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Biology (General), QH301-705.5 |
| الوصف: | In the present study, a bivalent vaccine against Pasteurella multocida and rabbit hemorrhagic disease virus (RHDV) was formulated with Montanide™ ISA70 oil adjuvant (Seppic, Paris, France). Its efficacy was evaluated and compared to similar monovalent preparations and commercially available monovalent vaccines. White new Zeeland rabbit groups (n = 10) received 2 successive doses of the tested vaccines and were challenged 2 weeks after 2nd dose with Pasteurella multocida and RHDV or either pathogens according to their vaccination schedule. Challenged not-vaccinated group of rabbits (n = 10) was included as a control. The bivalent and monovalent ISA70 preparations were found stable, safe, sterile, pure and of low viscosity. Group 3 (GP3) which received bivalent vaccine showed the highest antibody geometric mean titers against Pasteurella multocida and RHDV evaluated by ELISA and hemagglutination inhibition (HI) respectively. Following virulent challenge; Gp3 rabbits were 90% protected from challenge over other groups that showed 80% protection. Detection of either pathogen in the livers of dead and euthanized rabbits had failed except for non-vaccinated controls. The bivalent vaccine candidate was fully protective. Immunization against both pathogens can be achieved by single vaccination. Keywords: Pasteurellosis, RHDV, Vaccine, Rabbits, Bivalent, Montanide ISA70 |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1319-562X |
| Relation: | http://www.sciencedirect.com/science/article/pii/S1319562X19303420; https://doaj.org/toc/1319-562X; https://doaj.org/article/d57c2ab236334ac78c3576407b5d8961 |
| DOI: | 10.1016/j.sjbs.2019.12.042 |
| الاتاحة: | https://doi.org/10.1016/j.sjbs.2019.12.042 https://doaj.org/article/d57c2ab236334ac78c3576407b5d8961 |
| رقم الانضمام: | edsbas.2E63BBEE |
| قاعدة البيانات: | BASE |
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