Academic Journal
不同工艺制备的CLD/siRNA纳米粒体外性质差异性研究（英文） ; In vitro comparative evaluation of three CLD/siRNA nanoplexes prepared by different processes
| العنوان: | 不同工艺制备的CLD/siRNA纳米粒体外性质差异性研究（英文） ; In vitro comparative evaluation of three CLD/siRNA nanoplexes prepared by different processes |
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| المؤلفون: | 邱崇, 崔仕贺, 孙晶, 王坚成 |
| المساهمون: | 北京大学医学部药学院分子药剂学与新释药系统北京市重点实验室 |
| المصدر: | CSCD ; 知网 |
| بيانات النشر: | Journal of Chinese Pharmaceutical Sciences |
| سنة النشر: | 2016 |
| المجموعة: | Peking University Institutional Repository (PKU IR) / 北京大学机构知识库 |
| مصطلحات موضوعية: | 小干扰RNA, 递送, 纳米粒, 制备工艺, 细胞摄取, siRNA, Delivery, Nanoparticles, Preparation, Cellular uptake |
| الوصف: | 在本研究中,选择一种双子型阳离子脂材(CLD)为递送载体,用于阐明CLD/siRNA纳米粒(CLD/siRNA NPs)的"复合物特性"。使用不同的工艺方法制备了三种类型的CLD/siRNA纳米粒(AT NPs,HT NPs和MT NPs):AT工艺(siRNA溶液和预先制备好的CLD空白纳米粒混合)、HT工艺(siRNA溶液水化CLD薄膜)和MT工艺(在涡旋作用下,CLD乙醇溶液滴入siRNA溶液),从粒径、电位、形貌、siRNA保护效果、细胞毒性、细胞摄取能力和靶向mRNA的下调作用等方面展开了一系列对比性评价研究。在优化N/P=10/1的条件下,使用不同方法制备的三种CLD/siRNA纳. ; In this study, a gemini-like cationic lipid (CLD) was used as the carrier to study the complexation features of CLD/siRNA nanoplexes (CLD/siRNA NPs). Three types of CLD/siRNA nanoplexes (named as AT NPs, HT NPs and MT NPs) were prepared by different processes: AT method (mixing siRNA solution with preformed CLD nanoparticles), HT method (hydrating a CLD thin film with siRNA solution), and MT method (dropping an ethanolic solution of CLD into siRNA solution under sonication). The particle size, zeta potential, morphology, siRNA protection, cytotoxicity, cellular uptake, and targeted mRNA down-regulation were studied. At the optimal N/P ratio of 10, the sizes of the three CLD/siRNA NPs were MT NPs ((222.3±19.1) nm)> HT NPs ((105.7±1.31) nm)>AT NPs ((91.8±1.75) nm). Different nanostructures were formed despite the fact that they were composed of the same components. Furthermore, the TEM images indicated that different morphologies were found in the three NPs, indicating that the nanoplexes were assembled by different mechanisms. Among the three NPs, the cell uptake capacity were as follows: AT NPs>MT NPs>HT NPs, whereas the silencing levels on epidermal growth factor receptor (EGFR) in HeLa cells were MT NPs>AT NPs>HT NPs. Based on the above results, we hypothesized that the different preparation processes resulted in nanostructures with varying biological effects. Therefore, we believe that structural optimization of siRNA nanoplexes is essential in achieving better siRNA encapsulation, protection, and gene silencing efficiency. ; National Basic Research Program of China (973 program),the National Natural Science Foundation of China,Programs of Ministry of Education of China ; 中国科技核心期刊(ISTIC) ... |
| نوع الوثيقة: | journal/newspaper |
| اللغة: | Chinese |
| تدمد: | 1003-1057 |
| Relation: | Journal of Chinese Pharmaceutical Sciences.2016,660-668.; 1499821; http://hdl.handle.net/20.500.11897/453549 |
| الاتاحة: | https://hdl.handle.net/20.500.11897/453549 |
| رقم الانضمام: | edsbas.2D5FDF3E |
| قاعدة البيانات: | BASE |
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