Conference
Identificaton of DNA markers associated with trypsin inhibitor genes in grass pea (Lathyrus sativus sp.) and bean (Phaseolus vulgaris sp.) ; Identifikacija DNK markera povezanih sa genima inhibitora tripsina u sastrici (Lathyrus sativus sp.) i pasulju (Phaseolus vulgaris sp.)
| العنوان: | Identificaton of DNA markers associated with trypsin inhibitor genes in grass pea (Lathyrus sativus sp.) and bean (Phaseolus vulgaris sp.) ; Identifikacija DNK markera povezanih sa genima inhibitora tripsina u sastrici (Lathyrus sativus sp.) i pasulju (Phaseolus vulgaris sp.) |
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| المؤلفون: | Sinkovič, Lovro, Pipan, Barbara, Meglič, Vladimir, Isakov, Milada, Ilić, Aleksandra, Nagl, Nevena |
| المصدر: | Proceedings, 28th International Eco-Conference, 13th Safe Food, 25-27 September 2024, Novi Sad, Serbia |
| بيانات النشر: | Ekološki pokret Novog Sada |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | Grain legumes, Bowman-Birk inhibitor, Kunitz trypsin inhibitor, PCR primers, zrna mahunarki, PCA prajmeri |
| الوصف: | Grain legumes, including the grass pea (Lathyrus sativus) and the common bean (Phaseolus vulgaris), represent an inexpensive source of protein. However, they contain a number of antinutritional compounds, including protease inhibitors, which impair the availability of nutrients. The objective of this study was to identify and validate functional DNA markers associated with trypsin inhibitor genes in grass pea and common bean germplasm. The suitability of nine DNA markers for PCR amplification was evaluated under a range of temperature conditions and reaction mixtures in order to identify the optimal methodology. The trypsin inhibition genes Sas1 and Lat2_BBI were identified as species-specific only to the genome of Lathyrus sativus, while Sas2, Pas1, Pas2, Lat1_BBI, P_BBI, P_KTI and G1_KTI were found to be transferable and polymorphic between the genomes of Phaseolus vulgaris and Lathyrus sativus. The methodology developed will facilitate the routine analysis of these markers in the aforementioned grain legume germplasm. ; Zrna mahunarki, uključujući sastricu (Lathirus sativus) i obični pasulj (Phaseolus vulgaris), predstavljaju jeftin izvor proteina. Međutim, sadrže niz antinutritivnih jedinjenja, uključujući inhibitore proteaze, koji smanjuju dostupnost hranljivih materija. Cilj ove studije je bio da se identifikuju i validiraju funkcionalni DNK markeri povezani sa genima inhibitora tripsina u germplazmi graška i pasulja. Pogodnost devet DNK markera za PCR amplifikaciju je procenjena u nizu temperaturnih uslova i reakcionih smeša kako bi se identifikovala optimalna metodologija. Geni inhibicije tripsina Sas1 i Lat2_BBI identifikovani su kao specifični samo za genom Lathirus sativus, dok je za Sas2, Pas1, Pas2, Lat1_BBI, P_BBI, P_KTI i G1_KTI utvrđeno da su prenosivi i polimorfni između genoma vrusulga Phiseolus Lathirus. sativus. Razvijena metodologija će olakšati rutinsku analizu ovih markera u prethodno pomenutoj germplazmi mahunarki. |
| نوع الوثيقة: | conference object |
| اللغة: | English |
| Relation: | Bilateral project Slovenia - Serbia (337-00-110/2023-05/42) Trypsin inhibitors’ activity in grain legumes, 2023-2025; info:eu-repo/grantAgreement/ScienceFundRS/Prizma2023_BT/6691/RS//; https://fiver.ifvcns.rs/handle/123456789/4905; http://fiver.ifvcns.rs/bitstream/id/12908/bitstream_12908.pdf; https://hdl.handle.net/21.15107/rcub_fiver_4905 |
| الاتاحة: | https://fiver.ifvcns.rs/handle/123456789/4905 http://fiver.ifvcns.rs/bitstream/id/12908/bitstream_12908.pdf https://hdl.handle.net/21.15107/rcub_fiver_4905 |
| Rights: | openAccess ; https://creativecommons.org/licenses/by/4.0/ ; BY |
| رقم الانضمام: | edsbas.2784E0C3 |
| قاعدة البيانات: | BASE |
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