Academic Journal
Spatial mapping of metals in tissue-sections using combination of mass-spectrometry and histology through image registration
| العنوان: | Spatial mapping of metals in tissue-sections using combination of mass-spectrometry and histology through image registration |
|---|---|
| المؤلفون: | Anýz, J., Vysloužilová, L., Vaculovič, T., Tvrdoňová, M., Kanický, V., Haase, H., Horák, V. (Vratislav), Štěpánková, O., Heger, Z., Adam, V. |
| سنة النشر: | 2017 |
| المجموعة: | The Czech Academy of Sciences: Publication Activity (ASEP) / Akademie věd ČR - Publikační činnost |
| مصطلحات موضوعية: | melanoma patients, mixed effects models, bearing Libechov minipig |
| الوصف: | We describe a new procedure for the parallel mapping of selected metals in histologically characterized tissue samples. Mapping is achieved via image registration of digital data obtained from two neighbouring cryosections by scanning the first as a histological sample and subjecting the second to laser ablation inductively coupled plasma mass spectrometry. This computer supported procedure enables determination of the distribution and content of metals of interest directly in the chosen histological zones and represents a substantial improvement over the standard approach, which determines these values in tissue homogenates or whole tissue sections. The potential of the described procedure was demonstrated in a pilot study that analysed Zn and Cu levels in successive development stages of pig melanoma tissue using MeLiM (Melanoma-bearing-Libechov-Minipig) model. We anticipate that the procedure could be useful for a complex understanding of the role that the spatial distribution of metals plays within tissues affected by pathological states including cancer. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2045-2322 |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/28071735; urn:pissn: 2045-2322; urn:eissn: 2045-2322; http://hdl.handle.net/11104/0269536 |
| DOI: | 10.1038/srep40169 |
| الاتاحة: | https://doi.org/10.1038/srep40169 http://hdl.handle.net/11104/0269536 |
| رقم الانضمام: | edsbas.257F9C81 |
| قاعدة البيانات: | BASE |
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