Academic Journal
Total extract of Beta vulgaris var. cicla seeds versus its purified phenolic components: antioxidant activities and antiproliferative effects against colon cancer cells.
| العنوان: | Total extract of Beta vulgaris var. cicla seeds versus its purified phenolic components: antioxidant activities and antiproliferative effects against colon cancer cells. |
|---|---|
| المؤلفون: | Gennari L., Felletti M., Blasa M., Angelino D., Corallini A., Ninfali P., CELEGHINI, CLAUDIO |
| المساهمون: | Gennari, L., Felletti, M., Blasa, M., Angelino, D., Celeghini, Claudio, Corallini, A., Ninfali, P. |
| سنة النشر: | 2011 |
| المجموعة: | Università degli studi di Trieste: ArTS (Archivio della ricerca di Trieste) |
| مصطلحات موضوعية: | Beta vulgaris cicla seed, ORAC assay, antiproliferative activity, xylosylvitexin, human colon cancer cell, human fibroblast, cell cycle phases |
| الوصف: | Introduction – Beta vulgaris var. cicla (BV) leaves contain chemopreventive compounds that have been investigated for new drug discovery. These compounds belong to the family of the apigenin-glycosides. Since the leaves are seasonal products containing high percentages of water, they are easily degradable during storage in fresh conditions. To be stored they require a drying process, consuming time and a large amount of energy. The extraction of apigenin-glycosides may also be conveniently performed from BV seeds, which represent a stable and year-long available biomass. Objectives – The present reportwas undertaken to find a strategy of purification of bioactive flavonoids fromBV seeds and test their ability to inhibit proliferation both on human colon cancer (RKO) cells and normal human fibroblasts (HF). Materials and methods – The ethyl-acetate extract of BV seedswas fractionated on a Sephadex LH 20 column. A fraction of this extract, labeled as P4, exploited amarked antiproliferative activity on RKOcells.The components of P4were purified on an RP18 column chromatography and identified by HPLC-ESI-MS as 2,4,5-trihydroxybenzaldehyde, 2,5-dihydroxybenzaldehyde, vanillic acid, xylosylvitexin, glucopyranosyl-glucopyrasyl-rhamnetin and glucopyranosyl-xylosyl-rhamnetin. All of them were tested for cytostatic and cytotoxic activity on RKO and HF cells. Results – Xylosylvitexin exhibited the strongest antiproliferative activity on RKO cells, together with an enhancement of the apoptosis, an increase of cells in the G1 phase and a reduction of cells in the S phase; on the contrary, the proliferation of HFwas significantly stimulated. Conclusion – Xylosylvitexin is the main and more efficient chemopreventive compound in BV seeds, but the natural cocktail of molecules, represented by P4 fraction, showed a better compromise between the antiproliferative activity on RKOcells and the enhancement of HF proliferation. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | STAMPA |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/wos/WOS:000289824900012; volume:22; firstpage:272; lastpage:279; journal:PHYTOCHEMICAL ANALYSIS; http://hdl.handle.net/11368/2492347; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-79954467056 |
| DOI: | 10.1002/pca.1276 |
| الاتاحة: | http://hdl.handle.net/11368/2492347 https://doi.org/10.1002/pca.1276 |
| رقم الانضمام: | edsbas.1DE95D2C |
| قاعدة البيانات: | BASE |
| ResultId |
1 |
|---|---|
| Header |
edsbas BASE edsbas.1DE95D2C 773 3 Academic Journal academicJournal 772.654235839844 |
| PLink |
https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edsbas&AN=edsbas.1DE95D2C&custid=s6537998&authtype=sso |
| FullText |
Array
(
[Availability] => 0
)
Array ( [0] => Array ( [Url] => http://hdl.handle.net/11368/2492347# [Name] => EDS - BASE [Category] => fullText [Text] => View record in BASE [MouseOverText] => View record in BASE ) ) |
| Items |
Array
(
[Name] => Title
[Label] => Title
[Group] => Ti
[Data] => Total extract of Beta vulgaris var. cicla seeds versus its purified phenolic components: antioxidant activities and antiproliferative effects against colon cancer cells.
)
Array ( [Name] => Author [Label] => Authors [Group] => Au [Data] => <searchLink fieldCode="AR" term="%22Gennari+L%2E%22">Gennari L.</searchLink><br /><searchLink fieldCode="AR" term="%22Felletti+M%2E%22">Felletti M.</searchLink><br /><searchLink fieldCode="AR" term="%22Blasa+M%2E%22">Blasa M.</searchLink><br /><searchLink fieldCode="AR" term="%22Angelino+D%2E%22">Angelino D.</searchLink><br /><searchLink fieldCode="AR" term="%22Corallini+A%2E%22">Corallini A.</searchLink><br /><searchLink fieldCode="AR" term="%22Ninfali+P%2E%22">Ninfali P.</searchLink><br /><searchLink fieldCode="AR" term="%22CELEGHINI%2C+CLAUDIO%22">CELEGHINI, CLAUDIO</searchLink> ) Array ( [Name] => Author [Label] => Contributors [Group] => Au [Data] => Gennari, L.<br />Felletti, M.<br />Blasa, M.<br />Angelino, D.<br />Celeghini, Claudio<br />Corallini, A.<br />Ninfali, P. ) Array ( [Name] => DatePubCY [Label] => Publication Year [Group] => Date [Data] => 2011 ) Array ( [Name] => Subset [Label] => Collection [Group] => HoldingsInfo [Data] => Università degli studi di Trieste: ArTS (Archivio della ricerca di Trieste) ) Array ( [Name] => Subject [Label] => Subject Terms [Group] => Su [Data] => <searchLink fieldCode="DE" term="%22Beta+vulgaris+cicla+seed%22">Beta vulgaris cicla seed</searchLink><br /><searchLink fieldCode="DE" term="%22ORAC+assay%22">ORAC assay</searchLink><br /><searchLink fieldCode="DE" term="%22antiproliferative+activity%22">antiproliferative activity</searchLink><br /><searchLink fieldCode="DE" term="%22xylosylvitexin%22">xylosylvitexin</searchLink><br /><searchLink fieldCode="DE" term="%22human+colon+cancer+cell%22">human colon cancer cell</searchLink><br /><searchLink fieldCode="DE" term="%22human+fibroblast%22">human fibroblast</searchLink><br /><searchLink fieldCode="DE" term="%22cell+cycle+phases%22">cell cycle phases</searchLink> ) Array ( [Name] => Abstract [Label] => Description [Group] => Ab [Data] => Introduction – Beta vulgaris var. cicla (BV) leaves contain chemopreventive compounds that have been investigated for new drug discovery. These compounds belong to the family of the apigenin-glycosides. Since the leaves are seasonal products containing high percentages of water, they are easily degradable during storage in fresh conditions. To be stored they require a drying process, consuming time and a large amount of energy. The extraction of apigenin-glycosides may also be conveniently performed from BV seeds, which represent a stable and year-long available biomass. Objectives – The present reportwas undertaken to find a strategy of purification of bioactive flavonoids fromBV seeds and test their ability to inhibit proliferation both on human colon cancer (RKO) cells and normal human fibroblasts (HF). Materials and methods – The ethyl-acetate extract of BV seedswas fractionated on a Sephadex LH 20 column. A fraction of this extract, labeled as P4, exploited amarked antiproliferative activity on RKOcells.The components of P4were purified on an RP18 column chromatography and identified by HPLC-ESI-MS as 2,4,5-trihydroxybenzaldehyde, 2,5-dihydroxybenzaldehyde, vanillic acid, xylosylvitexin, glucopyranosyl-glucopyrasyl-rhamnetin and glucopyranosyl-xylosyl-rhamnetin. All of them were tested for cytostatic and cytotoxic activity on RKO and HF cells. Results – Xylosylvitexin exhibited the strongest antiproliferative activity on RKO cells, together with an enhancement of the apoptosis, an increase of cells in the G1 phase and a reduction of cells in the S phase; on the contrary, the proliferation of HFwas significantly stimulated. Conclusion – Xylosylvitexin is the main and more efficient chemopreventive compound in BV seeds, but the natural cocktail of molecules, represented by P4 fraction, showed a better compromise between the antiproliferative activity on RKOcells and the enhancement of HF proliferation. ) Array ( [Name] => TypeDocument [Label] => Document Type [Group] => TypDoc [Data] => article in journal/newspaper ) Array ( [Name] => Format [Label] => File Description [Group] => SrcInfo [Data] => STAMPA ) Array ( [Name] => Language [Label] => Language [Group] => Lang [Data] => English ) Array ( [Name] => NoteTitleSource [Label] => Relation [Group] => SrcInfo [Data] => info:eu-repo/semantics/altIdentifier/wos/WOS:000289824900012; volume:22; firstpage:272; lastpage:279; journal:PHYTOCHEMICAL ANALYSIS; http://hdl.handle.net/11368/2492347; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-79954467056 ) Array ( [Name] => DOI [Label] => DOI [Group] => ID [Data] => 10.1002/pca.1276 ) Array ( [Name] => URL [Label] => Availability [Group] => URL [Data] => http://hdl.handle.net/11368/2492347<br />https://doi.org/10.1002/pca.1276 ) Array ( [Name] => AN [Label] => Accession Number [Group] => ID [Data] => edsbas.1DE95D2C ) |
| RecordInfo |
Array
(
[BibEntity] => Array
(
[Identifiers] => Array
(
[0] => Array
(
[Type] => doi
[Value] => 10.1002/pca.1276
)
)
[Languages] => Array
(
[0] => Array
(
[Text] => English
)
)
[Subjects] => Array
(
[0] => Array
(
[SubjectFull] => Beta vulgaris cicla seed
[Type] => general
)
[1] => Array
(
[SubjectFull] => ORAC assay
[Type] => general
)
[2] => Array
(
[SubjectFull] => antiproliferative activity
[Type] => general
)
[3] => Array
(
[SubjectFull] => xylosylvitexin
[Type] => general
)
[4] => Array
(
[SubjectFull] => human colon cancer cell
[Type] => general
)
[5] => Array
(
[SubjectFull] => human fibroblast
[Type] => general
)
[6] => Array
(
[SubjectFull] => cell cycle phases
[Type] => general
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => Total extract of Beta vulgaris var. cicla seeds versus its purified phenolic components: antioxidant activities and antiproliferative effects against colon cancer cells.
[Type] => main
)
)
)
[BibRelationships] => Array
(
[HasContributorRelationships] => Array
(
[0] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Gennari L.
)
)
)
[1] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Felletti M.
)
)
)
[2] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Blasa M.
)
)
)
[3] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Angelino D.
)
)
)
[4] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Corallini A.
)
)
)
[5] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Ninfali P.
)
)
)
[6] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => CELEGHINI, CLAUDIO
)
)
)
[7] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Gennari, L.
)
)
)
[8] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Felletti, M.
)
)
)
[9] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Blasa, M.
)
)
)
[10] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Angelino, D.
)
)
)
[11] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Celeghini, Claudio
)
)
)
[12] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Corallini, A.
)
)
)
[13] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Ninfali, P.
)
)
)
)
[IsPartOfRelationships] => Array
(
[0] => Array
(
[BibEntity] => Array
(
[Dates] => Array
(
[0] => Array
(
[D] => 01
[M] => 01
[Type] => published
[Y] => 2011
)
)
[Identifiers] => Array
(
[0] => Array
(
[Type] => issn-locals
[Value] => edsbas
)
)
)
)
)
)
)
|
| IllustrationInfo |