التفاصيل البيبلوغرافية
| العنوان: |
Image1_Transforming Growth Factor-β3/Recombinant Human-like Collagen/Chitosan Freeze-Dried Sponge Primed With Human Periodontal Ligament Stem Cells Promotes Bone Regeneration in Calvarial Defect Rats.TIF |
| المؤلفون: |
Shiyi Huang (9125519), Fenglin Yu (9125522), Yating Cheng (523668), Yangfan Li (3954809), Yini Chen (5892689), Jianzhong Tang (8815298), Yu Bei (9125525), Qingxia Tang (10690269), Yueping Zhao (282775), Yadong Huang (108296), Qi Xiang (2146690) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Pharmacology, Basic Pharmacology, Clinical Pharmacology and Therapeutics, Clinical Pharmacy and Pharmacy Practice, Pharmaceutical Sciences, Pharmacogenomics, Toxicology (incl. Clinical Toxicology), Pharmacology and Pharmaceutical Sciences not elsewhere classified, periodontal ligament stem cells, transforming growth factor 3, stem cell therapy, skull bone defect repair, freeze-dried sponge |
| الوصف: |
Patients with a skull defect are at risk of developing cerebrospinal fluid leakage and ascending bacterial meningitis at >10% per year. However, treatment with stem cells has brought great hope to large-area cranial defects. Having found that transforming growth factor (TGF)-β3 can promote the osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs), we designed a hybrid TGF-β3/recombinant human-like collagen recombinant human collagen/chitosan (CS) freeze-dried sponge (TRFS) loading hPDLSCs (TRFS-h) to repair skull defects in rats. CFS with 2% CS was selected based on the swelling degree, water absorption, and moisture retention. The CS freeze-dried sponge (CFS) formed a porous three-dimensional structure, as observed by scanning electron microscopy. In addition, cytotoxicity experiments and calcein-AM/PI staining showed that TRFS had a good cellular compatibility and could be degraded completely at 90 days in the implantation site. Furthermore, bone healing was evaluated using micro-computed tomography in rat skull defect models. The bone volume and bone volume fraction were higher in TRFS loaded with hPDLSCs (TRFS-h) group than in the controls (p < 0.01, vs. CFS or TRFS alone). The immunohistochemical results indicated that the expression of Runx2, BMP-2, and collagen-1 (COL Ⅰ) in cells surrounding bone defects in the experimental group was higher than those in the other groups (p < 0.01, vs. CFS or TRFS alone). Taken together, hPDLSCs could proliferate and undergo osteogenic differentiation in TRFS (p < 0.05), and TRFS-h accelerated bone repair in calvarial defect rats. Our research revealed that hPDLSCs could function as seeded cells for skull injury, and their osteogenic differentiation could be accelerated by TGF-β3. This represents an effective therapeutic strategy for restoring traumatic defects of the skull. |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/Image1_Transforming_Growth_Factor-_3_Recombinant_Human-like_Collagen_Chitosan_Freeze-Dried_Sponge_Primed_With_Human_Periodontal_Ligament_Stem_Cells_Promotes_Bone_Regeneration_in_Calvarial_Defect_Rats_TIF/14472336 |
| DOI: |
10.3389/fphar.2021.678322.s001 |
| الاتاحة: |
https://doi.org/10.3389/fphar.2021.678322.s001 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.1BEFB877 |
| قاعدة البيانات: |
BASE |