Academic Journal
Low myoglobin concentration in skeletal muscle of elite cyclists is associated with low mRNA expression levels
| العنوان: | Low myoglobin concentration in skeletal muscle of elite cyclists is associated with low mRNA expression levels |
|---|---|
| المؤلفون: | Jacobs, Nina, Mos, Daniek, Bloemers, Frank W., van der Laarse, Willem J., Jaspers, Richard T., van der Zwaard, Stephan |
| المصدر: | Jacobs , N , Mos , D , Bloemers , F W , van der Laarse , W J , Jaspers , R T & van der Zwaard , S 2023 , ' Low myoglobin concentration in skeletal muscle of elite cyclists is associated with low mRNA expression levels ' , European Journal of Applied Physiology , vol. 123 , no. 7 , pp. 1469-1478 . https://doi.org/10.1007/s00421-023-05161-z |
| سنة النشر: | 2023 |
| الوصف: | Myoglobin is essential for oxygen transport to the muscle fibers. However, measurements of myoglobin (Mb) protein concentrations within individual human muscle fibers are scarce. Recent observations have revealed surprisingly low Mb concentrations in elite cyclists, however it remains unclear whether this relates to Mb translation, transcription and/or myonuclear content. The aim was to compare Mb concentration, Mb messenger RNA (mRNA) expression levels and myonuclear content within muscle fibers of these elite cyclists with those of physically-active controls. Muscle biopsies were obtained from m. vastus lateralis in 29 cyclists and 20 physically-active subjects. Mb concentration was determined by peroxidase staining for both type I and type II fibers, Mb mRNA expression level was determined by quantitative PCR and myonuclear domain size (MDS) was obtained by immunofluorescence staining. Average Mb concentrations (mean ± SD: 0.38 ± 0.04 mM vs. 0.48 ± 0.19 mM; P = 0.014) and Mb mRNA expression levels (0.067 ± 0.019 vs. 0.088 ± 0.027; P = 0.002) were lower in cyclists compared to controls. In contrast, MDS and total RNA per mg muscle were not different between groups. Interestingly, in cyclists compared to controls, Mb concentration was only lower for type I fibers (P < 0.001), but not for type II fibers (P > 0.05). In conclusion, the lower Mb concentration in muscle fibers of elite cyclists is partly explained by lower Mb mRNA expression levels per myonucleus and not by a lower myonuclear content. It remains to be determined whether cyclists may benefit from strategies that upregulate Mb mRNA expression levels, particularly in type I fibers, to enhance their oxygen supply. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1007/s00421-023-05161-z |
| الاتاحة: | https://research.vumc.nl/en/publications/c2b8f998-5056-491e-85bc-1b070e045063 https://doi.org/10.1007/s00421-023-05161-z http://www.scopus.com/inward/record.url?scp=85149342428&partnerID=8YFLogxK |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.16CDA874 |
| قاعدة البيانات: | BASE |
| ResultId |
1 |
|---|---|
| Header |
edsbas BASE edsbas.16CDA874 961 3 Academic Journal academicJournal 960.976379394531 |
| PLink |
https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edsbas&AN=edsbas.16CDA874&custid=s6537998&authtype=sso |
| FullText |
Array
(
[Availability] => 0
)
Array ( [0] => Array ( [Url] => https://research.vumc.nl/en/publications/c2b8f998-5056-491e-85bc-1b070e045063# [Name] => EDS - BASE [Category] => fullText [Text] => View record in BASE [MouseOverText] => View record in BASE ) ) |
| Items |
Array
(
[Name] => Title
[Label] => Title
[Group] => Ti
[Data] => Low myoglobin concentration in skeletal muscle of elite cyclists is associated with low mRNA expression levels
)
Array ( [Name] => Author [Label] => Authors [Group] => Au [Data] => <searchLink fieldCode="AR" term="%22Jacobs%2C+Nina%22">Jacobs, Nina</searchLink><br /><searchLink fieldCode="AR" term="%22Mos%2C+Daniek%22">Mos, Daniek</searchLink><br /><searchLink fieldCode="AR" term="%22Bloemers%2C+Frank+W%2E%22">Bloemers, Frank W.</searchLink><br /><searchLink fieldCode="AR" term="%22van+der+Laarse%2C+Willem+J%2E%22">van der Laarse, Willem J.</searchLink><br /><searchLink fieldCode="AR" term="%22Jaspers%2C+Richard+T%2E%22">Jaspers, Richard T.</searchLink><br /><searchLink fieldCode="AR" term="%22van+der+Zwaard%2C+Stephan%22">van der Zwaard, Stephan</searchLink> ) Array ( [Name] => TitleSource [Label] => Source [Group] => Src [Data] => Jacobs , N , Mos , D , Bloemers , F W , van der Laarse , W J , Jaspers , R T & van der Zwaard , S 2023 , ' Low myoglobin concentration in skeletal muscle of elite cyclists is associated with low mRNA expression levels ' , European Journal of Applied Physiology , vol. 123 , no. 7 , pp. 1469-1478 . https://doi.org/10.1007/s00421-023-05161-z ) Array ( [Name] => DatePubCY [Label] => Publication Year [Group] => Date [Data] => 2023 ) Array ( [Name] => Abstract [Label] => Description [Group] => Ab [Data] => Myoglobin is essential for oxygen transport to the muscle fibers. However, measurements of myoglobin (Mb) protein concentrations within individual human muscle fibers are scarce. Recent observations have revealed surprisingly low Mb concentrations in elite cyclists, however it remains unclear whether this relates to Mb translation, transcription and/or myonuclear content. The aim was to compare Mb concentration, Mb messenger RNA (mRNA) expression levels and myonuclear content within muscle fibers of these elite cyclists with those of physically-active controls. Muscle biopsies were obtained from m. vastus lateralis in 29 cyclists and 20 physically-active subjects. Mb concentration was determined by peroxidase staining for both type I and type II fibers, Mb mRNA expression level was determined by quantitative PCR and myonuclear domain size (MDS) was obtained by immunofluorescence staining. Average Mb concentrations (mean ± SD: 0.38 ± 0.04 mM vs. 0.48 ± 0.19 mM; P = 0.014) and Mb mRNA expression levels (0.067 ± 0.019 vs. 0.088 ± 0.027; P = 0.002) were lower in cyclists compared to controls. In contrast, MDS and total RNA per mg muscle were not different between groups. Interestingly, in cyclists compared to controls, Mb concentration was only lower for type I fibers (P < 0.001), but not for type II fibers (P > 0.05). In conclusion, the lower Mb concentration in muscle fibers of elite cyclists is partly explained by lower Mb mRNA expression levels per myonucleus and not by a lower myonuclear content. It remains to be determined whether cyclists may benefit from strategies that upregulate Mb mRNA expression levels, particularly in type I fibers, to enhance their oxygen supply. ) Array ( [Name] => TypeDocument [Label] => Document Type [Group] => TypDoc [Data] => article in journal/newspaper ) Array ( [Name] => Language [Label] => Language [Group] => Lang [Data] => English ) Array ( [Name] => DOI [Label] => DOI [Group] => ID [Data] => 10.1007/s00421-023-05161-z ) Array ( [Name] => URL [Label] => Availability [Group] => URL [Data] => https://research.vumc.nl/en/publications/c2b8f998-5056-491e-85bc-1b070e045063<br />https://doi.org/10.1007/s00421-023-05161-z<br />http://www.scopus.com/inward/record.url?scp=85149342428&partnerID=8YFLogxK ) Array ( [Name] => Copyright [Label] => Rights [Group] => Cpyrght [Data] => info:eu-repo/semantics/openAccess ) Array ( [Name] => AN [Label] => Accession Number [Group] => ID [Data] => edsbas.16CDA874 ) |
| RecordInfo |
Array
(
[BibEntity] => Array
(
[Identifiers] => Array
(
[0] => Array
(
[Type] => doi
[Value] => 10.1007/s00421-023-05161-z
)
)
[Languages] => Array
(
[0] => Array
(
[Text] => English
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => Low myoglobin concentration in skeletal muscle of elite cyclists is associated with low mRNA expression levels
[Type] => main
)
)
)
[BibRelationships] => Array
(
[HasContributorRelationships] => Array
(
[0] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Jacobs, Nina
)
)
)
[1] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Mos, Daniek
)
)
)
[2] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Bloemers, Frank W.
)
)
)
[3] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => van der Laarse, Willem J.
)
)
)
[4] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Jaspers, Richard T.
)
)
)
[5] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => van der Zwaard, Stephan
)
)
)
)
[IsPartOfRelationships] => Array
(
[0] => Array
(
[BibEntity] => Array
(
[Dates] => Array
(
[0] => Array
(
[D] => 01
[M] => 01
[Type] => published
[Y] => 2023
)
)
[Identifiers] => Array
(
[0] => Array
(
[Type] => issn-locals
[Value] => edsbas
)
[1] => Array
(
[Type] => issn-locals
[Value] => edsbas.oa
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => $2
[Type] => main
)
)
)
)
)
)
)
|
| IllustrationInfo |