IL-6, IL-8 and TNF production by cytokine and lipopolysaccharide-stimulated human renal cortical epithelial cells in vitro
| العنوان: | IL-6, IL-8 and TNF production by cytokine and lipopolysaccharide-stimulated human renal cortical epithelial cells in vitro |
|---|---|
| المؤلفون: | E J, von Asmuth, M A, Dentener, M, Ceska, W A, Buurman |
| المصدر: | European Cytokine Network. 5(3):301-310 |
| سنة النشر: | 1994 |
| مصطلحات موضوعية: | Lipopolysaccharides, Kidney Cortex, Interleukin-6, Tumor Necrosis Factor-alpha, Indomethacin, Interleukin-8, Epithelial Cells, Fibroblasts, Epithelium, Tacrolimus, Pregnenediones, Cyclosporine, Cytokines, Humans, Tetradecanoylphorbol Acetate, Endothelium, Vascular, Budesonide, Cells, Cultured |
| الوصف: | The capacity of renal epithelial cells to produce IL-6, IL-8 and TNF was investigated. Cultures of explanted human renal cortical epithelial cells (RCEC) were established, and cytokine-release and mRNA expression by these cells were measured. IL-6, IL-8 and TNF release were measured after stimulation with IL-1 beta TNF-alpha, LPS and the phorbol esther PMA. All these agents were found to induce increased release of the three cytokines. Whilst no spontaneous TNF-release occurred, IL-6 and IL-8 were continuously released by non-stimulated RCEC cultures. IL-1 beta was the most potent trigger, enhancing both RCEC cytokine release and expression of IL-6, IL-8 and TNF mRNA. Indomethacin, budesonide, cyclosporin and FK 506 were tested for their influence on RCEC cytokine release. Only the steroid budesonide appeared to reduce both spontaneous and IL-1 beta induced cytokine release. Our data demonstrate stimulus specific release of IL-6, IL-8 and TNF by RCEC, and suggest that cytokine cell-to-cell communication may be important in regulating inflammatory processes in the kidney. |
| اللغة: | English |
| تدمد: | 1148-5493 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::d3eb9dc3a9404d3ab1e3f508ad2776f6 http://www.ncbi.nlm.nih.gov/pubmed/7524716 |
| Rights: | RESTRICTED |
| رقم الانضمام: | edsair.pmid.dedup....d3eb9dc3a9404d3ab1e3f508ad2776f6 |
| قاعدة البيانات: | OpenAIRE |
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