Microfluidic-based 18F-labeling of biomolecules for immuno-positron emission tomography
| العنوان: | Microfluidic-based 18F-labeling of biomolecules for immuno-positron emission tomography |
|---|---|
| المؤلفون: | Kan, Liu, Eric J, Lepin, Ming-Wei, Wang, Feng, Guo, Wei-Yu, Lin, Yi-Chun, Chen, Shannon J, Sirk, Sebastian, Olma, Michael E, Phelps, Xing-Zhong, Zhao, Hsian-Rong, Tseng, R, Michael van Dam, Anna M, Wu, Clifton K-F, Shen |
| المصدر: | Molecular imaging. 10(3) |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Male, Fluorine Radioisotopes, Microfluidics, Mice, SCID, Hydrogen-Ion Concentration, Prostate-Specific Antigen, Article, Mice, Isotope Labeling, Positron-Emission Tomography, Animals, Humans, Tissue Distribution, Tomography, X-Ray Computed |
| الوصف: | Methods for tagging biomolecules with fluorine 18 as immuno-positron emission tomography (immunoPET) tracers require tedious optimization of radiolabeling conditions and can consume large amounts of scarce biomolecules. We describe an improved method using a digital microfluidic droplet generation (DMDG) chip, which provides computer-controlled metering and mixing of 18F tag, biomolecule, and buffer in defined ratios, allowing rapid scouting of reaction conditions in nanoliter volumes. The identified optimized conditions were then translated to bench-scale 18F labeling of a cancer-specific engineered antibody fragments, enabling microPET imaging of tumors in xenografted mice at 0.5 to 4 hours postinjection. |
| تدمد: | 1536-0121 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=pmid________::53bd72ffbbdda25d87c5337deed5de79 https://pubmed.ncbi.nlm.nih.gov/21496447 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.pmid..........53bd72ffbbdda25d87c5337deed5de79 |
| قاعدة البيانات: | OpenAIRE |
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