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AF10 Plays a Key Role in the Survival of Uncommitted Hematopoietic Cells

التفاصيل البيبلوغرافية
العنوان: AF10 Plays a Key Role in the Survival of Uncommitted Hematopoietic Cells
المؤلفون: Juan J. Arredondo, Margarita Cervera, Raquel Chamorro-Garcia
المصدر: PLoS ONE
PLoS ONE, Vol 7, Iss 12, p e51626 (2012)
Biblos-e Archivo. Repositorio Institucional de la UAM
instname
Digital.CSIC. Repositorio Institucional del CSIC
بيانات النشر: Public Library of Science, 2012.
سنة النشر: 2012
مصطلحات موضوعية: Cellular differentiation, lcsh:Medicine, Fluorescent Antibody Technique, Stem cell factor, Apoptosis, CXCR4, Cell Fate Determination, Hematologic Cancers and Related Disorders, Mice, RNA, Small Interfering, lcsh:Science, Cells, Cultured, Multidisciplinary, Leukemia, Reverse Transcriptase Polymerase Chain Reaction, Stem Cells, Cell Differentiation, Hematology, Flow Cytometry, Cell biology, Endothelial stem cell, medicine.anatomical_structure, Medicine, Stem cell, Research Article, Acute Myeloid Leukemia, Cell Survival, Blotting, Western, Chronic Myeloid Leukemia, Bone Marrow Cells, Biology, Real-Time Polymerase Chain Reaction, Leukemias, medicine, CD135, Animals, Humans, RNA, Messenger, Progenitor cell, Biología y Biomedicina, Cell Proliferation, lcsh:R, Hematopoietic Stem Cells, Molecular biology, Hematopoiesis, lcsh:Q, Bone marrow, AF10, Developmental Biology, Transcription Factors
الوصف: Hematopoiesis is a complex process regulated by both cell intrinsic and cell extrinsic factors. Alterations in the expression of critical genes during hematopoiesis can modify the balance between stem cell differentiation and proliferation, and may ultimately give rise to leukemia and other diseases. AF10 is a transcription factor that has been implicated in the development of leukemia following chromosomal rearrangements between the AF10 gene and one of at least two other genes, MLL and CALM. The link between AF10 and leukemia, together with the known interactions between AF10 and hematopoietic regulators, suggests that AF10 may be important in hematopoiesis and in leukemic transformation. Here we show that AF10 is important for proper hematopoietic differentiation. The induction of hematopoietic differentiation in both human hematopoietic cell lines and murine total bone marrow cells triggers a decrease of AF10 mRNA and protein levels, particularly in stem cells and multipotent progenitors. Gain- and loss-of-function studies demonstrate that over- or under-expression of AF10 leads to apoptotic cell death in stem cells and multipotent progenitors. We conclude that AF10 plays a key role in the maintenance of multipotent hematopoietic cells. © 2012 Chamorro-Garcia et al.
Work supported by grants 06/CSB/004 and CCG06-UAM/BIO-0322 from The Autonomous University of Madrid-CAM and by Fundación MMA.
وصف الملف: Application/pdf
اللغة: English
تدمد: 1932-6203
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::feb6db526aed45dc3bb544c2a4827e26
http://europepmc.org/articles/PMC3526614
Rights: OPEN
رقم الانضمام: edsair.doi.dedup.....feb6db526aed45dc3bb544c2a4827e26
قاعدة البيانات: OpenAIRE
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