Characterization of Corticotropin Receptors in Human Adrenocortical Cells*
| العنوان: | Characterization of Corticotropin Receptors in Human Adrenocortical Cells* |
|---|---|
| المؤلفون: | J. Ramachandran, Laura Stuve, Richard D. Catalano |
| المصدر: | The Journal of Clinical Endocrinology & Metabolism. 62:300-304 |
| بيانات النشر: | The Endocrine Society, 1986. |
| سنة النشر: | 1986 |
| مصطلحات موضوعية: | Adult, Male, endocrine system, medicine.medical_specialty, Endocrinology, Diabetes and Metabolism, Clinical Biochemistry, Receptors, Cell Surface, Stimulation, Peptide hormone, Biology, Biochemistry, Radioligand Assay, Endocrinology, Internal medicine, Extracellular, medicine, Humans, ACTH receptor, Binding site, Child, Receptor, Cells, Cultured, Adrenal cortex, Biochemistry (medical), Dissociation constant, Kinetics, medicine.anatomical_structure, Receptors, Corticotropin, Adrenal Cortex, Calcium, Female, hormones, hormone substitutes, and hormone antagonists |
| الوصف: | [125I-Tyr23,Phe2,Nle4]ACTH-(1-38) ([125I]ACTH analog), which is equipotent with ACTH, was used to characterize ACTH receptors in human adrenocortical cells. Adrenals were obtained from brain-dead patients at the time of renal harvest with permission. Binding of [125I]ACTH analog to human adrenocortical cells was highly specific, rapid, reversible, and saturable. Analysis of the inhibition of binding of [125I]ACTH analog by ACTH was compatible with a single class of binding sites with an apparent dissociation constant (Kd) of 1.6 nM and a mean binding capacity of 3560 sites/cell. Concentration-response curves for cAMP and cortisol production were shifted to the left of the binding curve, with ACTH concentrations required for half-maximal stimulation being 20- and 720-fold less, respectively, than those for binding. Extracellular calcium was essential for binding and stimulation of cAMP production. These results indicate that human adrenocortical cells contain a single class of ACTH receptors which are highly similar in affinity, capacity, and calcium requirement to those of rat adrenocortical cells, but differ from the rat receptors in the concentration of ACTH needed for cAMP generation. |
| تدمد: | 1945-7197 0021-972X |
| DOI: | 10.1210/jcem-62-2-300 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fa6d39a36a367d307dd8fb08651f5ecb https://doi.org/10.1210/jcem-62-2-300 |
| رقم الانضمام: | edsair.doi.dedup.....fa6d39a36a367d307dd8fb08651f5ecb |
| قاعدة البيانات: | OpenAIRE |
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