The use of mice in diabetes research: The impact of experimental protocols

التفاصيل البيبلوغرافية
العنوان: The use of mice in diabetes research: The impact of experimental protocols
المؤلفون: Ella R. P. White, Aileen King, Manasi Nandi, Lydia F. Daniels Gatward, Anna Roberts, Matilda R. Kennard
المصدر: Diabetic medicine : a journal of the British Diabetic AssociationREFERENCES. 38(12)
سنة النشر: 2021
مصطلحات موضوعية: Protocol (science), Blood Glucose, Glucose tolerance test, Biomedical Research, medicine.diagnostic_test, Continuous glucose monitoring, business.industry, Endocrinology, Diabetes and Metabolism, Blood Glucose Self-Monitoring, Psychological intervention, Glucose Tolerance Test, medicine.disease, Diabetes Mellitus, Experimental, Mice, Endocrinology, Consistency (negotiation), Risk analysis (engineering), Diabetes mellitus, Internal Medicine, medicine, Diabetes Mellitus, Glucose homeostasis, Animals, Full disclosure, business
الوصف: Mice are used extensively in preclinical diabetes research to model various aspects of blood glucose homeostasis. Careful experimental design is vital for maximising welfare and improving reproducibility of data. Alongside decisions regarding physiological characteristics of the animal cohort (e.g., sex, strain and age), experimental protocols must also be carefully considered. This includes choosing relevant end points of interest and understanding what information they can provide and what their limitations are. Details of experimental protocols must, therefore, be carefully planned during the experimental design stage, especially considering the impact of researcher interventions on preclinical end points. Indeed, in line with the 3Rs of animal research, experiments should be refined where possible to maximise welfare. The role of welfare may be particularly pertinent in preclinical diabetes research as blood glucose concentrations are directly altered by physiological stress responses. Despite the potential impact of variations in experimental protocols, there is distinct lack of standardisation and consistency throughout the literature with regards to several experimental procedures including fasting, cage changing and glucose tolerance test protocol. This review firstly highlights practical considerations with regard to the choice of end points in preclinical diabetes research and the potential for novel technologies such as continuous glucose monitoring and glucose clamping techniques to improve data resolution. The potential influence of differing experimental protocols and in vivo procedures on both welfare and experimental outcomes is then discussed with focus on standardisation, consistency and full disclosure of methods.
تدمد: 1464-5491
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f71cb6faece2c524d34fa46489a3f141
https://pubmed.ncbi.nlm.nih.gov/34596274
Rights: OPEN
رقم الانضمام: edsair.doi.dedup.....f71cb6faece2c524d34fa46489a3f141
قاعدة البيانات: OpenAIRE