Effects of a low-intensity resistance exercise program on serum miR-630, miR-5703, and Fractalkine/CX3CL1 expressions in subjects with No exercise habits: A preliminary study
| العنوان: | Effects of a low-intensity resistance exercise program on serum miR-630, miR-5703, and Fractalkine/CX3CL1 expressions in subjects with No exercise habits: A preliminary study |
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| المؤلفون: | Tsubasa Tsutsumi, Takumi Kawaguchi, Masafumi Bekki, Takahiro Sugimoto, Naoto Shiba, Hiroo Matsuse, Koji Hara, Souhei Iwanaga, Takuji Torimura, Syunji Koya, Tatsuya Kanto, Keisuke Hirota, Sachiyo Yoshio, Dan Nakano, Ryuki Hashida |
| المصدر: | Hepatology research : the official journal of the Japan Society of HepatologyREFERENCES. 51(7) |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | medicine.medical_specialty, Hepatology, business.industry, Microarray analysis techniques, medicine.medical_treatment, Wnt signaling pathway, Metabolic equivalent, 03 medical and health sciences, 0302 clinical medicine, Infectious Diseases, Endocrinology, Cytokine, 030220 oncology & carcinogenesis, Internal medicine, microRNA, Myokine, medicine, 030211 gastroenterology & hepatology, KEGG, business, CX3CL1 |
| الوصف: | Aims Exercise is effective for the prevention of liver cancer. Exercise exerts biological effects through the regulation of microRNAs (miRNAs) and cytokines/myokines. We aimed to investigate the effects of low-intensity resistance exercise on serum miRNA and cytokine/myokine expressions in subjects with no exercise habits. Methods We enrolled seven male subjects with no exercise habits in this prospective before-after study. All subjects performed a low-intensity resistance exercise program (three metabolic equivalents, approximately 20 min/session). Serum miRNA expressions were evaluated using microarrays. We performed Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of differentially expressed miRNAs before and after exercise. Serum cytokine/myokine expressions were evaluated using a multiplex panel. Results All subjects completed the exercise program with no adverse events. In the microarray analysis, seven miRNAs showed a significant change between before and after exercise. Of these, microRNA (miR)-630 and miR-5703 showed a >1.5-fold increase (miR-630: 40.7 vs. 69.3 signal intensity, P=0.0133; miR-5703: 30.7 vs. 55.9 signal intensity, P=0.0051). KEGG pathway enrichment analysis showed that miR-630- and miR-5703-related genes were enriched in 37 and 5 pathways, including transforming growth factor-beta and Wnt signaling pathways, respectively. In the multiplex analysis, twelve cytokines/myokines showed significant alteration after exercise compared to before exercise. Of these, fractalkine/CX3CL1 showed the most significant up-regulation by exercise (94.5 vs. 109.1 pg/mL, P=0.0017). Conclusions A low-intensity resistance exercise program was associated with upregulation of serum miR-630, miR-5703, and fractalkine/CX3CL1 expressions in subjects with no exercise habits. Thus, even low-intensity exercise may alter miRNA and cytokine/myokine expressions in humans. This article is protected by copyright. All rights reserved. |
| تدمد: | 1386-6346 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee076c02a9fb2426d3d6aacd1ab5ba48 https://pubmed.ncbi.nlm.nih.gov/34014020 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....ee076c02a9fb2426d3d6aacd1ab5ba48 |
| قاعدة البيانات: | OpenAIRE |
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