أعرض في EDS

Autophagy protects murine preputial glands against premature aging, and controls their sebum phospholipid and pheromone profile

التفاصيل البيبلوغرافية
العنوان: Autophagy protects murine preputial glands against premature aging, and controls their sebum phospholipid and pheromone profile
المؤلفون: Samuele Zoratto, Maria Buchberger, Michaela Sochorová, Barbara Lengauer, Bahar Golabi, Florian Gruber, Leopold Eckhart, Dragan Copic, Christopher Kremslehner, Heidemarie Rossiter, Martin Direder, Michael Mildner, Erwin Tschachler, Ionela-Mariana Nagelreiter, Martina Marchetti-Deschmann, Supawadee Sukseree, Veronika Mlitz
المصدر: Autophagy
بيانات النشر: Informa UK Limited, 2021.
سنة النشر: 2021
مصطلحات موضوعية: Sebaceous gland, Perilipin 2, Preputial gland, Phospholipid, Phosphatidylserines, Perilipin-2, Pheromones, Mice, chemistry.chemical_compound, Lipid droplet, Autophagy, medicine, Animals, Molecular Biology, Phospholipids, Phosphatidylethanolamine, biology, Holocrine, Aging, Premature, Lipid metabolism, Cell Biology, Cell biology, Sebum, medicine.anatomical_structure, chemistry, biology.protein, lipids (amino acids, peptides, and proteins), Research Paper
الوصف: Preputial glands are large lipid and hormone secreting sebaceous organs of mice, and present a convenient model for the investigation of biological processes in sebocytes. Suppression of ATG7-dependent macroautophagy/autophagy in epithelial cells of murine skin causes enlargement of hair follicle-associated sebaceous glands and alters the lipid profile of sebum. We have now extended these studies to the preputial glands and find that autophagy significantly delays the onset of age-related ductal ectasia, influences lipid droplet morphology and contributes to the complete dissolution of the mature sebocytes during holocrine secretion. Single cell RNA sequencing showed that many genes involved in lipid metabolism and oxidative stress response were downregulated in immature and mature epithelial cells of ATG7-deficient glands. When analyzing the lipid composition of control and mutant glands, we found that levels of all phospholipid classes, except choline plasmalogen, were decreased in the mutant glands, with a concomitant accumulation of diacyl glycerides. Mass spectrometric imaging (MSI) demonstrated that phospholipid species, specifically the dominant phosphatidylcholine (PC 34:1), were decreased in immature and mature sebocytes. In addition, we found a strong reduction in the amounts of the pheromone, palmityl acetate. Thus, autophagy in the preputial gland is not only important for homeostasis of the gland as a whole and an orderly breakdown of cells during holocrine secretion, but also regulates phospholipid and fatty acid metabolism, as well as pheromone production. ABBREVIATIONS: ATG7: autophagy related 7; BODIPY: boron dipyrromethene; DAG: diacyl glycerides; DBI: diazepam binding inhibitor; GFP: green fluorescent protein; KRT14: keratin 14; HPLC-MS: high performance liquid chromatography-mass spectrometry; LD: lipid droplet; MAP1LC3/LC3: microtubule-associated protein 1 light chain 3; MSI: mass spectrometric imaging; ORO: Oil Red O; PC: phosphatidylcholine; PE: phosphatidylethanolamine; PG: preputial gland; PLIN2: perilipin 2; PtdIns: phosphatidylinositol; PL: phospholipids; POPC: 1-palmitoyl-2-oleoyl-PC; PS: phosphatidylserine; qRT-PCR: quantitative reverse transcribed PCR; SG: sebaceous gland; scRNAseq: single-cell RNA sequencing; TAG: triacylglycerides; TLC: thin layer chromatography.
تدمد: 1554-8635
1554-8627
DOI: 10.1080/15548627.2021.1966716
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee0152a7bddb1fc76bf47a079e6814d6
https://doi.org/10.1080/15548627.2021.1966716
Rights: OPEN
رقم الانضمام: edsair.doi.dedup.....ee0152a7bddb1fc76bf47a079e6814d6
قاعدة البيانات: OpenAIRE
ResultId 1
Header edsair
OpenAIRE
edsair.doi.dedup.....ee0152a7bddb1fc76bf47a079e6814d6
884
3

unknown
883.651977539063
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edsair&AN=edsair.doi.dedup.....ee0152a7bddb1fc76bf47a079e6814d6&custid=s6537998&authtype=sso
FullText Array ( [Availability] => 0 )
Array ( [0] => Array ( [Url] => https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee0152a7bddb1fc76bf47a079e6814d6# [Name] => EDS - OpenAIRE [Category] => fullText [Text] => View record in OpenAIRE [MouseOverText] => View record in OpenAIRE ) )
Items Array ( [Name] => Title [Label] => Title [Group] => Ti [Data] => Autophagy protects murine preputial glands against premature aging, and controls their sebum phospholipid and pheromone profile )
Array ( [Name] => Author [Label] => Authors [Group] => Au [Data] => <searchLink fieldCode="AR" term="%22Samuele+Zoratto%22">Samuele Zoratto</searchLink><br /><searchLink fieldCode="AR" term="%22Maria+Buchberger%22">Maria Buchberger</searchLink><br /><searchLink fieldCode="AR" term="%22Michaela+Sochorová%22">Michaela Sochorová</searchLink><br /><searchLink fieldCode="AR" term="%22Barbara+Lengauer%22">Barbara Lengauer</searchLink><br /><searchLink fieldCode="AR" term="%22Bahar+Golabi%22">Bahar Golabi</searchLink><br /><searchLink fieldCode="AR" term="%22Florian+Gruber%22">Florian Gruber</searchLink><br /><searchLink fieldCode="AR" term="%22Leopold+Eckhart%22">Leopold Eckhart</searchLink><br /><searchLink fieldCode="AR" term="%22Dragan+Copic%22">Dragan Copic</searchLink><br /><searchLink fieldCode="AR" term="%22Christopher+Kremslehner%22">Christopher Kremslehner</searchLink><br /><searchLink fieldCode="AR" term="%22Heidemarie+Rossiter%22">Heidemarie Rossiter</searchLink><br /><searchLink fieldCode="AR" term="%22Martin+Direder%22">Martin Direder</searchLink><br /><searchLink fieldCode="AR" term="%22Michael+Mildner%22">Michael Mildner</searchLink><br /><searchLink fieldCode="AR" term="%22Erwin+Tschachler%22">Erwin Tschachler</searchLink><br /><searchLink fieldCode="AR" term="%22Ionela-Mariana+Nagelreiter%22">Ionela-Mariana Nagelreiter</searchLink><br /><searchLink fieldCode="AR" term="%22Martina+Marchetti-Deschmann%22">Martina Marchetti-Deschmann</searchLink><br /><searchLink fieldCode="AR" term="%22Supawadee+Sukseree%22">Supawadee Sukseree</searchLink><br /><searchLink fieldCode="AR" term="%22Veronika+Mlitz%22">Veronika Mlitz</searchLink> )
Array ( [Name] => TitleSource [Label] => Source [Group] => Src [Data] => Autophagy )
Array ( [Name] => Publisher [Label] => Publisher Information [Group] => PubInfo [Data] => Informa UK Limited, 2021. )
Array ( [Name] => DatePubCY [Label] => Publication Year [Group] => Date [Data] => 2021 )
Array ( [Name] => Subject [Label] => Subject Terms [Group] => Su [Data] => <searchLink fieldCode="DE" term="%22Sebaceous+gland%22">Sebaceous gland</searchLink><br /><searchLink fieldCode="DE" term="%22Perilipin+2%22">Perilipin 2</searchLink><br /><searchLink fieldCode="DE" term="%22Preputial+gland%22">Preputial gland</searchLink><br /><searchLink fieldCode="DE" term="%22Phospholipid%22">Phospholipid</searchLink><br /><searchLink fieldCode="DE" term="%22Phosphatidylserines%22">Phosphatidylserines</searchLink><br /><searchLink fieldCode="DE" term="%22Perilipin-2%22">Perilipin-2</searchLink><br /><searchLink fieldCode="DE" term="%22Pheromones%22">Pheromones</searchLink><br /><searchLink fieldCode="DE" term="%22Mice%22">Mice</searchLink><br /><searchLink fieldCode="DE" term="%22chemistry%2Echemical%5Fcompound%22">chemistry.chemical_compound</searchLink><br /><searchLink fieldCode="DE" term="%22Lipid+droplet%22">Lipid droplet</searchLink><br /><searchLink fieldCode="DE" term="%22Autophagy%22">Autophagy</searchLink><br /><searchLink fieldCode="DE" term="%22medicine%22">medicine</searchLink><br /><searchLink fieldCode="DE" term="%22Animals%22">Animals</searchLink><br /><searchLink fieldCode="DE" term="%22Molecular+Biology%22">Molecular Biology</searchLink><br /><searchLink fieldCode="DE" term="%22Phospholipids%22">Phospholipids</searchLink><br /><searchLink fieldCode="DE" term="%22Phosphatidylethanolamine%22">Phosphatidylethanolamine</searchLink><br /><searchLink fieldCode="DE" term="%22biology%22">biology</searchLink><br /><searchLink fieldCode="DE" term="%22Holocrine%22">Holocrine</searchLink><br /><searchLink fieldCode="DE" term="%22Aging%2C+Premature%22">Aging, Premature</searchLink><br /><searchLink fieldCode="DE" term="%22Lipid+metabolism%22">Lipid metabolism</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+Biology%22">Cell Biology</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+biology%22">Cell biology</searchLink><br /><searchLink fieldCode="DE" term="%22Sebum%22">Sebum</searchLink><br /><searchLink fieldCode="DE" term="%22medicine%2Eanatomical%5Fstructure%22">medicine.anatomical_structure</searchLink><br /><searchLink fieldCode="DE" term="%22chemistry%22">chemistry</searchLink><br /><searchLink fieldCode="DE" term="%22biology%2Eprotein%22">biology.protein</searchLink><br /><searchLink fieldCode="DE" term="%22lipids+%28amino+acids%2C+peptides%2C+and+proteins%29%22">lipids (amino acids, peptides, and proteins)</searchLink><br /><searchLink fieldCode="DE" term="%22Research+Paper%22">Research Paper</searchLink> )
Array ( [Name] => Abstract [Label] => Description [Group] => Ab [Data] => Preputial glands are large lipid and hormone secreting sebaceous organs of mice, and present a convenient model for the investigation of biological processes in sebocytes. Suppression of ATG7-dependent macroautophagy/autophagy in epithelial cells of murine skin causes enlargement of hair follicle-associated sebaceous glands and alters the lipid profile of sebum. We have now extended these studies to the preputial glands and find that autophagy significantly delays the onset of age-related ductal ectasia, influences lipid droplet morphology and contributes to the complete dissolution of the mature sebocytes during holocrine secretion. Single cell RNA sequencing showed that many genes involved in lipid metabolism and oxidative stress response were downregulated in immature and mature epithelial cells of ATG7-deficient glands. When analyzing the lipid composition of control and mutant glands, we found that levels of all phospholipid classes, except choline plasmalogen, were decreased in the mutant glands, with a concomitant accumulation of diacyl glycerides. Mass spectrometric imaging (MSI) demonstrated that phospholipid species, specifically the dominant phosphatidylcholine (PC 34:1), were decreased in immature and mature sebocytes. In addition, we found a strong reduction in the amounts of the pheromone, palmityl acetate. Thus, autophagy in the preputial gland is not only important for homeostasis of the gland as a whole and an orderly breakdown of cells during holocrine secretion, but also regulates phospholipid and fatty acid metabolism, as well as pheromone production. ABBREVIATIONS: ATG7: autophagy related 7; BODIPY: boron dipyrromethene; DAG: diacyl glycerides; DBI: diazepam binding inhibitor; GFP: green fluorescent protein; KRT14: keratin 14; HPLC-MS: high performance liquid chromatography-mass spectrometry; LD: lipid droplet; MAP1LC3/LC3: microtubule-associated protein 1 light chain 3; MSI: mass spectrometric imaging; ORO: Oil Red O; PC: phosphatidylcholine; PE: phosphatidylethanolamine; PG: preputial gland; PLIN2: perilipin 2; PtdIns: phosphatidylinositol; PL: phospholipids; POPC: 1-palmitoyl-2-oleoyl-PC; PS: phosphatidylserine; qRT-PCR: quantitative reverse transcribed PCR; SG: sebaceous gland; scRNAseq: single-cell RNA sequencing; TAG: triacylglycerides; TLC: thin layer chromatography. )
Array ( [Name] => ISSN [Label] => ISSN [Group] => ISSN [Data] => 1554-8635<br />1554-8627 )
Array ( [Name] => DOI [Label] => DOI [Group] => ID [Data] => 10.1080/15548627.2021.1966716 )
Array ( [Name] => URL [Label] => Access URL [Group] => URL [Data] => <link linkTarget="URL" linkTerm="https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee0152a7bddb1fc76bf47a079e6814d6" linkWindow="_blank">https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee0152a7bddb1fc76bf47a079e6814d6</link><br /><link linkTarget="URL" linkTerm="https://doi.org/10.1080/15548627.2021.1966716" linkWindow="_blank">https://doi.org/10.1080/15548627.2021.1966716</link> )
Array ( [Name] => Copyright [Label] => Rights [Group] => Cpyrght [Data] => OPEN )
Array ( [Name] => AN [Label] => Accession Number [Group] => ID [Data] => edsair.doi.dedup.....ee0152a7bddb1fc76bf47a079e6814d6 )
RecordInfo Array ( [BibEntity] => Array ( [Identifiers] => Array ( [0] => Array ( [Type] => doi [Value] => 10.1080/15548627.2021.1966716 ) ) [Languages] => Array ( [0] => Array ( [Text] => Undetermined ) ) [PhysicalDescription] => Array ( [Pagination] => Array ( [PageCount] => 15 [StartPage] => 1005 ) ) [Subjects] => Array ( [0] => Array ( [SubjectFull] => Sebaceous gland [Type] => general ) [1] => Array ( [SubjectFull] => Perilipin 2 [Type] => general ) [2] => Array ( [SubjectFull] => Preputial gland [Type] => general ) [3] => Array ( [SubjectFull] => Phospholipid [Type] => general ) [4] => Array ( [SubjectFull] => Phosphatidylserines [Type] => general ) [5] => Array ( [SubjectFull] => Perilipin-2 [Type] => general ) [6] => Array ( [SubjectFull] => Pheromones [Type] => general ) [7] => Array ( [SubjectFull] => Mice [Type] => general ) [8] => Array ( [SubjectFull] => chemistry.chemical_compound [Type] => general ) [9] => Array ( [SubjectFull] => Lipid droplet [Type] => general ) [10] => Array ( [SubjectFull] => Autophagy [Type] => general ) [11] => Array ( [SubjectFull] => medicine [Type] => general ) [12] => Array ( [SubjectFull] => Animals [Type] => general ) [13] => Array ( [SubjectFull] => Molecular Biology [Type] => general ) [14] => Array ( [SubjectFull] => Phospholipids [Type] => general ) [15] => Array ( [SubjectFull] => Phosphatidylethanolamine [Type] => general ) [16] => Array ( [SubjectFull] => biology [Type] => general ) [17] => Array ( [SubjectFull] => Holocrine [Type] => general ) [18] => Array ( [SubjectFull] => Aging, Premature [Type] => general ) [19] => Array ( [SubjectFull] => Lipid metabolism [Type] => general ) [20] => Array ( [SubjectFull] => Cell Biology [Type] => general ) [21] => Array ( [SubjectFull] => Cell biology [Type] => general ) [22] => Array ( [SubjectFull] => Sebum [Type] => general ) [23] => Array ( [SubjectFull] => medicine.anatomical_structure [Type] => general ) [24] => Array ( [SubjectFull] => chemistry [Type] => general ) [25] => Array ( [SubjectFull] => biology.protein [Type] => general ) [26] => Array ( [SubjectFull] => lipids (amino acids, peptides, and proteins) [Type] => general ) [27] => Array ( [SubjectFull] => Research Paper [Type] => general ) ) [Titles] => Array ( [0] => Array ( [TitleFull] => Autophagy protects murine preputial glands against premature aging, and controls their sebum phospholipid and pheromone profile [Type] => main ) ) ) [BibRelationships] => Array ( [HasContributorRelationships] => Array ( [0] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Samuele Zoratto ) ) ) [1] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Maria Buchberger ) ) ) [2] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Michaela Sochorová ) ) ) [3] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Barbara Lengauer ) ) ) [4] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Bahar Golabi ) ) ) [5] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Florian Gruber ) ) ) [6] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Leopold Eckhart ) ) ) [7] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Dragan Copic ) ) ) [8] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Christopher Kremslehner ) ) ) [9] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Heidemarie Rossiter ) ) ) [10] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Martin Direder ) ) ) [11] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Michael Mildner ) ) ) [12] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Erwin Tschachler ) ) ) [13] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Ionela-Mariana Nagelreiter ) ) ) [14] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Martina Marchetti-Deschmann ) ) ) [15] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Supawadee Sukseree ) ) ) [16] => Array ( [PersonEntity] => Array ( [Name] => Array ( [NameFull] => Veronika Mlitz ) ) ) ) [IsPartOfRelationships] => Array ( [0] => Array ( [BibEntity] => Array ( [Dates] => Array ( [0] => Array ( [D] => 07 [M] => 09 [Type] => published [Y] => 2021 ) ) [Identifiers] => Array ( [0] => Array ( [Type] => issn-print [Value] => 15548635 ) [1] => Array ( [Type] => issn-print [Value] => 15548627 ) [2] => Array ( [Type] => issn-locals [Value] => edsair ) [3] => Array ( [Type] => issn-locals [Value] => edsairFT ) ) [Numbering] => Array ( [0] => Array ( [Type] => volume [Value] => 18 ) ) [Titles] => Array ( [0] => Array ( [TitleFull] => Autophagy [Type] => main ) ) ) ) ) ) )
IllustrationInfo