Comprehensive analysis of the peanut allergome combining 2-DE gel-based and gel-free proteomics
| العنوان: | Comprehensive analysis of the peanut allergome combining 2-DE gel-based and gel-free proteomics |
|---|---|
| المؤلفون: | Luigia Di Stasio, Gianfranco Mamone, Gianluca Picariello, Pasquale Ferranti, Maria Adalgisa Nicolai, Salvatore De Caro |
| المساهمون: | Mamone, Gianfranco, DI STASIO, Luigia, De Caro, Salvatore, Picariello, Gianluca, Nicolai, MARIA ADALGISA, Ferranti, Pasquale |
| المصدر: | Food Research International. 116:1059-1065 |
| بيانات النشر: | Elsevier BV, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Proteomics, Arachis, Proteome, Peanut, Allergome, High MW aggregates, Two-dimensional electrophoresis, Shotgun proteomics, 030309 nutrition & dietetics, Gel based, 03 medical and health sciences, 0404 agricultural biotechnology, Tandem Mass Spectrometry, Two dimensional electrophoresis, Nuts, Electrophoresis, Gel, Two-Dimensional, Peanut Hypersensitivity, Gene, Chromatography, High Pressure Liquid, Plant Proteins, 0303 health sciences, Gel free proteomics, Chemistry, Membrane Proteins, food and beverages, 04 agricultural and veterinary sciences, Antigens, Plant, 040401 food science, Biochemistry, Relevant information, Food Science |
| الوصف: | In this work, we explored the “deep” seed peanut proteome by using both two dimensional electrophoresis (2- DE)-based analysis run under reducing and non-reducing condition (protein-centric) and LC-MS/MS gel-free proteomic (peptide-centric). The former approach allowed to identify high molecular weight disulfide-linked Ara h 1 and Ara h 3 heteroligomers and Ara h 1 homoligomers linked through covalent bonds other than disulfides. The occurrence of these protein complexes revealed natural interactions between Ara(s) subunits with a possible involvement in the allergenic potential of peanut. The second approach, also referred to as shot-gun proteomics, allowed the identification of 149 gene products, including low-abundance proteins escaped the 2-DE detection. Interestingly, we identified 60 proteins never catalogued previously. The complementary exploitation of two proteomic approaches enabled the access to new relevant information about the complexity of the peanut proteome, with special emphasis to the complement of allergens (allergome). |
| تدمد: | 0963-9969 |
| DOI: | 10.1016/j.foodres.2018.09.045 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e9bd79ff6f80556b72397c282c1a2ea0 https://doi.org/10.1016/j.foodres.2018.09.045 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....e9bd79ff6f80556b72397c282c1a2ea0 |
| قاعدة البيانات: | OpenAIRE |
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