Models and methods for in vitro testing of hepatic gap junctional communication
| العنوان: | Models and methods for in vitro testing of hepatic gap junctional communication |
|---|---|
| المؤلفون: | Michaël Maes, Mathieu Vinken, IVTD VUB, Joost Willebrords, Sara Crespo Yanguas |
| المساهمون: | Pharmaceutical and Pharmacological Sciences, Connexin Signalling Research Group, Liver Connexin and Pannexin Research Group, Experimental in vitro toxicology and dermato-cosmetology |
| المصدر: | Toxicology in Vitro. 30:569-577 |
| بيانات النشر: | Elsevier BV, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | connexin, Cell signaling, Tumor Promoters, Connexin, Cell Communication, Biology, liver, Toxicology, Article, gap junction, Humans, Gap junction activity, Dimethyl Sulfoxide, Epigenetics, Cells, Cultured, nongenotoxic carcinogen, Liver cell, Gap junction, Gap Junctions, Hep G2 Cells, General Medicine, Coculture Techniques, In vitro, Cell biology, Intercellular communication, in vitro model, Hepatocytes |
| الوصف: | Inherent to their pivotal roles in controlling all aspects of the liver cell life cycle, hepatocellular gap junctions are frequently disrupted upon impairment of the homeostatic balance, as occurs during liver toxicity. Hepatic gap junctions, which are mainly built up by connexin32, are specifically targeted by tumor promoters and epigenetic carcinogens. This renders inhibition of gap junction functionality a suitable indicator for the in vitro detection of nongenotoxic hepatocarcinogenicity. The establishment of a reliable liver gap junction inhibition assay for routine in vitro testing purposes requires a cellular system in which gap junctions are expressed at an in vivo-like level as well as an appropriate technique to probe gap junction activity. Both these models and methods are discussed in the current paper, thereby focusing on connexin32-based gap junctions. |
| تدمد: | 0887-2333 |
| DOI: | 10.1016/j.tiv.2015.09.024 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::df58e05ae97809bdf97fc89a20ddc5ab https://doi.org/10.1016/j.tiv.2015.09.024 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....df58e05ae97809bdf97fc89a20ddc5ab |
| قاعدة البيانات: | OpenAIRE |
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